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120 条记录 (耗时 0.027 秒)

    Quantitative analysis of biochemical compound utilizing electrochemical reaction
    11.
    发明授权
    Quantitative analysis of biochemical compound utilizing electrochemical reaction 失效
    利用电化学反应定量分析生化化合物

    公开(公告)号：US06261780B1

    公开(公告)日：2001-07-17

    申请号：US09477973

    申请日：2000-01-05

    申请人： Masashi Ogawa ,  Shigeori Takenaka ,  Makoto Takagi

    发明人： Masashi Ogawa ,  Shigeori Takenaka ,  Makoto Takagi

    IPC分类号： C12Q168

    CPC分类号： C12Q1/60 ,  C12Q1/001 ,  C12Q1/26 ,  C12Q1/54 ,  C12Q1/6825 ,  C12Q2563/173 ,  C12Q2545/114 ,  C12Q2527/137

    摘要： A method for quantitatively analyzing a biochemical compound such as glucose or cholesterol is performed by bringing the biochemical compound into contact with double stranded DNA fragments which are fixed onto a surface of an electrode at their one terminals and in which electrochemically active threading intercalators are intercalated, in an aqueous medium under application of electric potential to the electrode in the presence of an oxidase capable of oxidizing the biochemical compound and becoming a reduced oxidase, and detecting an electric current flowing between the electrode and another electrode placed in the aqueous medium.

    摘要翻译： 通过使生物化学化合物与固定在电极表面上的双链DNA片段接触并进行电化学活性穿插嵌入剂的插入，进行定量分析生物化学化合物如葡萄糖或胆固醇的方法， 在能够氧化生物化学化合物并变成还原氧化酶的氧化酶存在下向电极施加电位的水性介质中，并且检测在电极和置于水性介质中的另一电极之间流动的电流。

    Dry analytical element containing ampholyte
    15.
    发明授权
    Dry analytical element containing ampholyte 失效
    含两性电解质的干分析元素

    公开(公告)号：US5709837A

    公开(公告)日：1998-01-20

    申请号：US545233

    申请日：1995-10-19

    申请人： Toshihiro Mori ,  Masashi Ogawa ,  Yoshikazu Amano

    发明人： Toshihiro Mori ,  Masashi Ogawa ,  Yoshikazu Amano

    IPC分类号： G01N31/22 ,  G01N33/52 ,  G01N33/70 ,  G01N21/17

    CPC分类号： G01N33/525 ,  Y10T436/147777 ,  Y10T436/171538

    摘要： A dry analytical element comprising at least one water-permeable reagent layer and a porous spreading layer laminated in this order on a support, wherein at least one layer which is the water-permeable reagent layer, the porous spreading layer or an optional water-permeable layer contains a water-soluble polybasic amino carboxylic acid which is an ampholyte. By applying an analytical element of the invention, an analyte can be measured at a high sensitivity using a small amount of an enzyme, etc., and shelf file can be extended.

    摘要翻译： 一种干燥分析元件，其包括依次层叠在载体上的至少一个透水性试剂层和多孔扩散层，其中至少一层作为透水性试剂层，多孔扩散层或任选的透水性 层含有两性电解质的水溶性多元氨基羧酸。 通过应用本发明的分析元件，可以使用少量的酶等以高灵敏度测量分析物，并且可以延长搁架文件。

    Medium for electrophoresis
    16.
    发明授权
    Medium for electrophoresis 失效
    电泳培养基

    公开(公告)号：US4806434A

    公开(公告)日：1989-02-21

    申请号：US752938

    申请日：1985-07-08

    申请人： Masashi Ogawa

    发明人： Masashi Ogawa

    IPC分类号： C08F267/10 ,  G01N27/447 ,  B32B27/08

    CPC分类号： G01N27/44747 ,  C08F267/10 ,  Y10S524/916 ,  Y10T428/31725

    摘要： A medium for electrophoresis comprising an aqueous acrylamide gel formed by crosslinking polymerization of an acrylamide compound and an acrylamide copolymer having at least one specifically selected constitutional repeating unit in the presence of water. The medium may contain a water-soluble polymer and agarose. The medium may contain a modifier such as an anionic surfactant or a compound having at least one carbamoyl group.

    摘要翻译： 一种用于电泳的介质，其包含通过丙烯酰胺化合物的交联聚合和在水存在下具有至少一个具体选择的结构重复单元的丙烯酰胺共聚物形成的丙烯酰胺凝胶水溶液。 介质可能含有水溶性聚合物和琼脂糖。 介质可以含有改性剂，例如阴离子表面活性剂或具有至少一个氨基甲酰基的化合物。

    Element for electrophoresis
    17.
    发明授权
    Element for electrophoresis 失效
    电泳元件

    公开(公告)号：US4737259A

    公开(公告)日：1988-04-12

    申请号：US948401

    申请日：1986-12-29

    申请人： Masashi Ogawa ,  Masakazu Hashiue ,  Yuzo Mizobuchi

    发明人： Masashi Ogawa ,  Masakazu Hashiue ,  Yuzo Mizobuchi

    IPC分类号： G01N27/447 ,  C07K3/14

    CPC分类号： G01N27/44747 ,  Y10T428/31765

    摘要： An element for electrophoresis suitably employable for electrophoresis of biopolymers such as proteins, as well as for the determination of the base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic support, a nonconductive metal oxide layer, and an electrophoresis medium layer comprising an aqueous polyacrylamide gel formed by crosslinking polymerization of an acrylamide compound and a crosslinking agent in the presence of water, which are superposed in this order. The electrophoresis medium layer may contain a water-soluble polymer and agarose. The medium layer may contain a modifier such as an anionic surfactant, formamide or urea.

    摘要翻译： 适用于生物聚合物如蛋白质电泳的电泳元件，以及用于测定DNA，RNA，其片段及其衍生物的碱基序列，其包含塑料载体，非导电金属氧化物层和 电泳介质层，其包含通过在水存在下交联聚合丙烯酰胺化合物和交联剂而形成的聚丙烯酰胺凝胶水溶液，其以该顺序重叠。 电泳介质层可含有水溶性聚合物和琼脂糖。 中间层可以含有改性剂，例如阴离子表面活性剂，甲酰胺或脲。

    Element for electrophoresis
    18.
    发明授权
    Element for electrophoresis 失效
    电泳元件

    公开(公告)号：US4718998A

    公开(公告)日：1988-01-12

    申请号：US52579

    申请日：1987-05-19

    申请人： Masashi Ogawa ,  Naohiko Sugimoto

    发明人： Masashi Ogawa ,  Naohiko Sugimoto

    IPC分类号： G01N27/447 ,  G01N27/26 ,  B01D17/06

    CPC分类号： G01N27/44747 ,  Y10T428/31507 ,  Y10T428/31797 ,  Y10T428/31913 ,  Y10T428/3192

    摘要： An element for electrophoresis suitably employable for analysis of biopolymers such as proteins, as well as for determination of base sequence of DNA, RNA, their fragments, and their derivatives, which comprises a plastic film, a layer for electrophoresis comprising an aqueous polyacrylamide gel, an adhesive layer containing gelatin and optionally a film-forming polymer and/or fine particles, and a plastic film.

    摘要翻译： 适用于分析生物聚合物如蛋白质的电泳元件，以及用于测定DNA，RNA，其片段及其衍生物的碱基序列，其包括塑料膜，用于电泳的层，其包含聚丙烯酰胺凝胶水溶液， 含有明胶和任选的成膜聚合物和/或细颗粒的粘合剂层和塑料膜。

    Silver halide photographic light-sensitive material
    20.
    发明授权
    Silver halide photographic light-sensitive material 失效
    卤化银照相感光材料

    公开(公告)号：US4460680A

    公开(公告)日：1984-07-17

    申请号：US411384

    申请日：1982-08-25

    申请人： Masashi Ogawa ,  Kunio Ishigaki ,  Nobuyuki Iwasaki ,  Taku Nakamura

    发明人： Masashi Ogawa ,  Kunio Ishigaki ,  Nobuyuki Iwasaki ,  Taku Nakamura

    IPC分类号： G03C1/30 ,  C08F20/00 ,  C08F20/10 ,  C08F20/52 ,  C08F28/00 ,  C08F220/00 ,  C08F220/04 ,  C08F220/06 ,  C08F220/38 ,  C08F220/54 ,  C08F226/02 ,  G03C1/76

    CPC分类号： G03C1/7614 ,  Y10S430/162

    摘要： A silver halide photographic light-sensitive material comprising a support having thereon at least one light-sensitive silver halide emulsion layer and a light-insensitive uppermost layer is disclosed. The light-insensitive uppermost layer has a melting time longer than that of the light-sensitive silver halide emulsion layer and the thickness of the light-insensitive uppermost layer is from 1.3 .mu.m to 5.0 .mu.m. The silver halide photographic light-sensitive material has an improved covering power and results in remarkably low degree of reticulation. Furthermore, when the material is used it results in a reduced amount of scum being formed in the processing solution.

    摘要翻译： 公开了一种卤化银照相感光材料，其包括其上具有至少一个感光卤化银乳剂层和不透光的最上层的载体。 光敏最上层的熔融时间比感光卤化银乳剂层的熔化时间长，光敏最上层的厚度为1.3μm〜5.0μm。 卤化银摄影感光材料具有改进的覆盖力并导致明显低的网状度。 此外，当使用该材料时，会导致在处理溶液中形成的浮渣数量减少。