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    Process for the separation of double-stranded/single-stranded nucleic acid structures
    11.
    发明授权
    Process for the separation of double-stranded/single-stranded nucleic acid structures 失效
    分离双链/单链核酸结构的方法

    公开(公告)号：US06180778B2

    公开(公告)日：2001-01-30

    申请号：US08687610

    申请日：1996-08-08

    Applicant: Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

    Inventor： Helge Bastian ,  Simone Gauch ,  Metin Colpan ,  Petra Feuser

    IPC: C07H2100

    CPC classification number: C12N15/1006 ,  C12N15/101 ,  C12Q1/6806

    Abstract: The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.

    Abstract translation: 本发明涉及通过将所述核酸整体同时吸收到矿物载体上，然后将其分离成双链和单链核酸的单链和单链核酸部分，将核酸混合物色谱分离成双链和单链核酸部分 通过分批洗脱或通过选择性地将液体样品的双链或单链核酸吸收到矿物载体，以及用于执行根据本发明的方法的溶液和试剂盒来代替。

    Process for the depletion or removal of endotoxins
    13.
    发明授权
    Process for the depletion or removal of endotoxins 失效
    内毒素消耗或去除的过程

    公开(公告)号：US5747663A

    公开(公告)日：1998-05-05

    申请号：US687522

    申请日：1996-09-30

    Applicant: Metin Colpan ,  Peter Moritz ,  Joachim Schorr

    Inventor： Metin Colpan ,  Peter Moritz ,  Joachim Schorr

    IPC: B01J20/281 ,  A61K31/70 ,  A61K31/7088 ,  A61K48/00 ,  A61P35/00 ,  A61P43/00 ,  B01J20/26 ,  B01J41/04 ,  C07H1/08 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C12N15/10 ,  C12N15/87 ,  G01N30/88 ,  C12P19/34 ,  C12N1/06

    CPC classification number: B01D15/363 ,  B01D15/3804 ,  C12N15/101 ,  C12N15/87 ,  Y10S435/81 ,  Y10S435/82 ,  Y10S436/808 ,  Y10T436/10

    Abstract: A process is disclosed for reducing or removing endotoxins from compositions containing therapeutic active substances extracted from natural sources by genetic engineering and/or biotechnology. For that purpose, the compositions are treated with chromatographic materials. The natural sources are disintegrated, the thus obtained fractions are, if required, centrifuged, filtered or treated using affinity chromatography methods, the fractions are pre-incubated in an aqueous salt solution and detergents, are treated with anion exchange materials, then washed with another salt solution. The active substances are eluted from the anion exchanger then further purified in a manner known per se.

    Abstract translation: PCT No.PCT / EP95 / 00391 Sec。 371日期1996年9月30日 102（e）1996年9月30日PCT提交1995年2月3日PCT公布。 WO95 / 21179 PCT公开号 日期1995年8月10日公开了一种通过基因工程和/或生物技术从含有从天然来源提取的治疗活性物质的组合物中还原或去除内毒素的方法。 为此，用色谱材料处理组合物。 将天然来源分解，如果需要，如此获得的级分用亲和层析方法离心，过滤或处理，将级分在盐水溶液和洗涤剂中预温育，用阴离子交换材料处理，然后用另一种 盐溶液。 活性物质从阴离子交换剂洗脱，然后以本身已知的方式进一步纯化。

    Device and a method for separating liquid samples
    15.
    发明授权
    Device and a method for separating liquid samples 失效
    装置和分离液体样品的方法

    公开(公告)号：US5264184A

    公开(公告)日：1993-11-23

    申请号：US671448

    申请日：1991-03-19

    Applicant: James E. Aysta ,  Metin Colpan

    Inventor： James E. Aysta ,  Metin Colpan

    IPC: B01D15/00 ,  B01D24/00 ,  B01D29/01 ,  B01D61/18 ,  B01L3/00 ,  B01L99/00 ,  C12M1/00 ,  C12Q1/68 ,  B01L11/00 ,  B01D29/00

    CPC classification number: B01L3/5025 ,  B01D61/18 ,  B01L3/50255 ,  B01L3/50855 ,  Y10T436/25375 ,  Y10T436/255

    Abstract: A device (10) for separating liquid samples has a sample container (12), bottom wall (20) of which has an outlet opening (22) provided therein. The outlet opening (22) is joined by an outlet spout (24) extending in the axial direction of the sample container (12). On bottom wall (20) there is a separation layer (28). Below sample container (12), a collecting container (32) is arranged abutting sample container (12) such that an exchange of air between the interior of the collecting chamber (32) and the environment is possible, yet an escape of liquid is largely inhibited. The contact surface between collecting chamber (32) and sample container (12) and end (26) of the outlet spout (24) through which the liquid is discharged, are axially spaced apart.

    Abstract translation: 用于分离液体样品的装置（10）具有样品容器（12），其底壁（20）具有设置在其中的出口（22）。 出口开口（22）通过在样品容器（12）的轴向方向上延伸的出口喷口（24）连接。 在底壁（20）上有分离层（28）。 在样本容器（12）的下面，收集容器（32）设置成邻接样本容器（12），使得可以在收集室（32）的内部和环境之间交换空气，但是液体逸出很大程度上 抑制。 收集室（32）和样品容器（12）之间的接触表面以及排出液体的出口喷口（24）的端部（26）轴向间隔开。

    Device for the isolation of cell components such as nucleic acids from natural sources
    19.
    发明授权
    Device for the isolation of cell components such as nucleic acids from natural sources 失效
    用于分离细胞组分如来自天然来源的核酸的装置

    公开(公告)号：US06609618B2

    公开(公告)日：2003-08-26

    申请号：US09900199

    申请日：2001-07-09

    Applicant: Metin Colpan

    Inventor： Metin Colpan

    IPC: B01D2900

    CPC classification number: C12N15/101 ,  B01D39/00 ,  C12N15/1017 ,  Y10T436/25375 ,  Y10T436/255

    Abstract: Described is a method of isolating cell components, such as nucleic acids, from natural sources by filtering a sample of the digested natural sources such as cells or cell fragments. The method is characterized in that the sample is passed through a filter, the pore size of which decreases in the direction of flow of the sample through the filter.

    Abstract translation: 描述了通过过滤消化的天然来源（例如细胞或细胞碎片）的样品从天然来源分离细胞成分（例如核酸）的方法。 该方法的特征在于，样品通过过滤器，其孔径在样品通过过滤器的流动方向上减小。

    Chromatographic purification and separation process for mixtures of nucleic acids
    20.
    发明授权
    Chromatographic purification and separation process for mixtures of nucleic acids 失效
    用于核酸混合物的色谱纯化和分离过程

    公开(公告)号：US06383393B1

    公开(公告)日：2002-05-07

    申请号：US08392882

    申请日：1996-03-15

    Applicant: Metin Colpan ,  Joachim Schorr ,  Ralf Herrmann ,  Petra Feuser

    Inventor： Metin Colpan ,  Joachim Schorr ,  Ralf Herrmann ,  Petra Feuser

    IPC: B01D1508

    CPC classification number: C12N15/101

    Abstract: A method for the purification and separation of nucleic acid mixtures by chromatography including adsorbing the nucleic acids to be separated and purified from a solution with a high concentration of salts (ionic strength) and/or a high concentration of alcohol on a substrate and subsequent desorbing from the substrate by means of a solution with lower concentration of salts (ionic strength).

    Abstract translation: 一种通过色谱法纯化和分离核酸混合物的方法，其包括将从高浓度盐（离子强度）和/或高浓度醇溶液中分离和纯化的核酸吸附在底物上并随后解吸 通过具有较低浓度盐的溶液（离子强度）从底物引发。

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