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公开(公告)号：US07510826B2
公开(公告)日：2009-03-31
申请号：US11412130
申请日：2006-04-27
Applicant: Metin Colpan , Peter Moritz , Joachim Schorr
Inventor： Metin Colpan , Peter Moritz , Joachim Schorr
CPC classification number: B01D15/363 , B01D15/3804 , C12N15/101 , C12N15/87 , Y10S435/81 , Y10S435/82 , Y10S436/808 , Y10T436/10 , G01N30/461
Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural sources are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.
Abstract translation: 从含有指定用于治疗用途的活性成分的制剂中消耗或除去内毒素的方法，其通过遗传工程和/或生物技术从天然来源获得，其中所述天然来源被裂解，所得的级分任选地离心， 用亲和层析法过滤或处理; 所述级分用盐水溶液和洗涤剂预先加热，用阴离子交换材料处理，然后用另一种盐溶液洗涤，活性成分从阴离子交换剂中洗脱，然后以本身已知的方式进一步纯化。
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2.发明授权Process for the preparation of endotoxin-free or endotoxin-depleted nucleic acids and/or oligonucleotides for gene therapy 有权用于制备用于基因治疗的无内毒素或缺乏内毒素的核酸和/或寡核苷酸的方法公开(公告)号：US06297371B1
公开(公告)日：2001-10-02
申请号：US09253702
申请日：1999-02-22
Applicant: Metin Colpan , Joachim Schorr , Peter Moritz
Inventor： Metin Colpan , Joachim Schorr , Peter Moritz
IPC: C07H2100
CPC classification number: B01D15/363 , B01D15/3804 , C12N15/101 , C12N15/87 , Y10S435/81 , Y10S435/82 , Y10S436/808 , Y10T436/10 , G01N30/461
Abstract: A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy wherein said nucleic acids and/or oligonucleotides are isolated or purified from an essentially biological source, characterized in that said essentially biological sources are lysed, the fractions obtained are optionally freed or depleted from the remainder of said biological sources by per se known mechanical methods, such as centrifugation, filtration; the fractions thus treated are subsequently treated with affinity chromatographic material or with inorganic chromatographic material for the removal of endotoxins; followed by isolation of said nucleic acids and/or oligonucleotides on an anion exchanger which is designed such that DNA begins to desorb from the anion exchanger only at an ionic strength corresponding to a sodium chloride solution of a concentration higher by at least 100 mM than one corresponding to the ionic strength at which RNA begins to desorb from the anion exchanger material.
Abstract translation: 用于分离和纯化用于基因治疗的核酸和/或寡核苷酸的方法，其中所述核酸和/或寡核苷酸从基本上生物来源分离或纯化，其特征在于基本上生物来源被裂解，获得的级分为 通过本身已知的机械方法，例如离心，过滤，任选地从所述生物源的剩余部分中释放或消耗;随后用亲和层析材料或用无机色谱材料处理如此处理的级分以除去内毒素; 然后在阴离子交换器上分离所述核酸和/或寡核苷酸，所述阴离子交换器被设计为使得DNA开始从阴离子交换剂解吸离子，其离子强度对应于浓度高于一个相应浓度至少100mM的氯化钠溶液 到RNA开始从阴离子交换材料解吸的离子强度。
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3.发明授权
公开(公告)号：US5990301A
公开(公告)日：1999-11-23
申请号：US687529
申请日：1996-10-18
Applicant: Metin Colpan , Joachim Schorr , Peter Moritz
Inventor： Metin Colpan , Joachim Schorr , Peter Moritz
CPC classification number: C12N15/87 , C12N15/101 , Y10S435/81
Abstract: A process for the isolation and purification of nucleic acids and/or oligonucleotides for use in gene therapy wherein nucleic acids and/or oligonucleotides are isolated or purified from an essentially biological source, characterized in thatessentially biological sources are lysed, the fractions obtained are optionally freed or depleted from the remainder of biological sources by per se known mechanical methods, such as centrifugation, filtration;the fractions thus treated are subsequently treated with affinity chromatographic material or with inorganic chromatographic material for the removal of endotoxins; followed byisolation of nucleic acids and/or oligonucleotides on an anion exchanger which is designed such that DNA begins to desorb from the anion exchanger only at an ionic strength corresponding to a sodium chloride solution of a concentration higher by at least 100 mM than one corresponding to the ionic strength at which RNA begins to desorb from the anion exchanger material.
Abstract translation: PCT No.PCT / EP95 / 00389 371日期：1996年10月18日 102（e）日期1996年10月18日PCT提交1995年2月3日PCT公布。 第WO95 / 21177号公报 日期1995年8月10日一种用于分离和纯化用于基因治疗的核酸和/或寡核苷酸的方法，其中从基本上生物来源分离或纯化核酸和/或寡核苷酸，其特征在于基本上生物来源被裂解， 通过本身已知的机械方法，例如离心，过滤，任选地从所述生物来源的其余部分中释放或消耗所得的级分。 随后用亲和层析材料或用无机色谱材料处理如此处理的级分以除去内毒素; 随后在阴离子交换器上分离核酸和/或寡核苷酸，其被设计为使得DNA开始从阴离子交换剂解吸，仅在对应于浓度高于一个浓度的至少100mM的氯化钠溶液的离子强度 到RNA开始从阴离子交换材料解吸的离子强度。
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公开(公告)号：US07109322B2
公开(公告)日：2006-09-19
申请号：US10254845
申请日：2002-09-26
Applicant: Metin Colpan , Peter Moritz , Joachim Schorr
Inventor： Metin Colpan , Peter Moritz , Joachim Schorr
CPC classification number: B01D15/363 , B01D15/3804 , C12N15/101 , C12N15/87 , Y10S435/81 , Y10S435/82 , Y10S436/808 , Y10T436/10 , G01N30/461
Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural source are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.
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5.发明授权
公开(公告)号：US5792651A
公开(公告)日：1998-08-11
申请号：US687530
申请日：1996-10-11
Applicant: Metin Colpan , Joachim Schorr
Inventor： Metin Colpan , Joachim Schorr
CPC classification number: C12N15/87 , C12N15/101
Abstract: Use of isopropanol in aqueous solutions for chromatographic isolation of nucleic acids for enhancing the transfection efficiency of the isolated nucleic acids in prokaryotic and eukaryotic cells.
Abstract translation: PCT No.PCT / EP95 / 00390 Sec。 371日期：1996年10月11日 102（e）日期1996年10月11日PCT提交1995年2月3日PCT公布。 WO95 / 21178 PCT出版物 日期1995年8月10日使用用于色谱分离核酸的水溶液中的异丙醇，用于提高分离的核酸在原核和真核细胞中的转染效率。
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Patent Agency Ranking
公开(公告)号：US5747663A
公开(公告)日：1998-05-05
申请号：US687522
申请日：1996-09-30
Applicant: Metin Colpan , Peter Moritz , Joachim Schorr
Inventor： Metin Colpan , Peter Moritz , Joachim Schorr
IPC: B01J20/281 , A61K31/70 , A61K31/7088 , A61K48/00 , A61P35/00 , A61P43/00 , B01J20/26 , B01J41/04 , C07H1/08 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/10 , C12N15/87 , G01N30/88 , C12P19/34 , C12N1/06
CPC classification number: B01D15/363 , B01D15/3804 , C12N15/101 , C12N15/87 , Y10S435/81 , Y10S435/82 , Y10S436/808 , Y10T436/10
Abstract: A process is disclosed for reducing or removing endotoxins from compositions containing therapeutic active substances extracted from natural sources by genetic engineering and/or biotechnology. For that purpose, the compositions are treated with chromatographic materials. The natural sources are disintegrated, the thus obtained fractions are, if required, centrifuged, filtered or treated using affinity chromatography methods, the fractions are pre-incubated in an aqueous salt solution and detergents, are treated with anion exchange materials, then washed with another salt solution. The active substances are eluted from the anion exchanger then further purified in a manner known per se.
Abstract translation: PCT No.PCT / EP95 / 00391 Sec。 371日期1996年9月30日 102（e）1996年9月30日PCT提交1995年2月3日PCT公布。 WO95 / 21179 PCT公开号 日期1995年8月10日公开了一种通过基因工程和/或生物技术从含有从天然来源提取的治疗活性物质的组合物中还原或去除内毒素的方法。 为此，用色谱材料处理组合物。 将天然来源分解，如果需要，如此获得的级分用亲和层析方法离心，过滤或处理，将级分在盐水溶液和洗涤剂中预温育，用阴离子交换材料处理，然后用另一种 盐溶液。 活性物质从阴离子交换剂洗脱，然后以本身已知的方式进一步纯化。
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公开(公告)号：US20060194304A1
公开(公告)日：2006-08-31
申请号：US11412130
申请日：2006-04-27
Applicant: Metin Colpan , Peter Moritz , Joachim Schorr
Inventor： Metin Colpan , Peter Moritz , Joachim Schorr
IPC: C02F3/34
CPC classification number: B01D15/363 , B01D15/3804 , C12N15/101 , C12N15/87 , Y10S435/81 , Y10S435/82 , Y10S436/808 , Y10T436/10 , G01N30/461
Abstract: A process for the depletion or removal of endotoxins from preparations containing active ingredients designated for therapeutical use which are obtained from natural sources by genetic engineering and/or biotechnology by treatment with chromatographic material wherein said natural sources are lysed, the fractions obtained are optionally centrifuged, filtrated or treated with affinity chromatographic methods; said fractions are preincubated with an aqueous salt solution and detergents, treated with anion exchange material and then washed with another salt solution, and the active ingredients are eluted from the anion exchanger, followed by further purification in a per se known manner.
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8.发明授权Chromatographic purification and separation process for mixtures of nucleic acids 失效用于核酸混合物的色谱纯化和分离过程公开(公告)号：US06383393B1
公开(公告)日：2002-05-07
申请号：US08392882
申请日：1996-03-15
Applicant: Metin Colpan , Joachim Schorr , Ralf Herrmann , Petra Feuser
Inventor： Metin Colpan , Joachim Schorr , Ralf Herrmann , Petra Feuser
IPC: B01D1508
CPC classification number: C12N15/101
Abstract: A method for the purification and separation of nucleic acid mixtures by chromatography including adsorbing the nucleic acids to be separated and purified from a solution with a high concentration of salts (ionic strength) and/or a high concentration of alcohol on a substrate and subsequent desorbing from the substrate by means of a solution with lower concentration of salts (ionic strength).
Abstract translation: 一种通过色谱法纯化和分离核酸混合物的方法，其包括将从高浓度盐（离子强度）和/或高浓度醇溶液中分离和纯化的核酸吸附在底物上并随后解吸 通过具有较低浓度盐的溶液（离子强度）从底物引发。
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9.发明授权公开(公告)号：US06946250B2
公开(公告)日：2005-09-20
申请号：US10000526
申请日：2001-12-04
Applicant: Helge Bastian , Simone Gauch , Metin Colpan , Petra Feuser
Inventor： Helge Bastian , Simone Gauch , Metin Colpan , Petra Feuser
IPC: C07H1/06 , B01J20/281 , B01J20/283 , B01J20/285 , C07H1/08 , C07H21/00 , C07H21/02 , C07H21/04 , C12N15/09 , C12N15/10 , C12Q1/68 , G01N30/26 , G01N30/88 , C12P19/34
CPC classification number: C12N15/1006 , C12N15/101 , C12Q1/6806
Abstract: The present invention pertains to a process for the chromatographic separation of nucleic acid mixtures into their double-stranded and single-stranded nucleic acid fractions by simultaneously absorbing said nucleic acids as a whole to a mineral support, followed by separation into double-stranded and single-stranded nucleic acids by fractional elution, or by selectively absorbing double-stranded or single-stranded nucleic acid of a liquid sample to a mineral support, as well as solutions and a kit for performing the process according to the invention.
Abstract translation: 本发明涉及通过将所述核酸整体同时吸收到矿物载体上，然后将其分离成双链和单链核酸的单链和单链核酸部分，将核酸混合物色谱分离成双链和单链核酸部分 通过分批洗脱或通过选择性地将液体样品的双链或单链核酸吸收到矿物载体，以及用于执行根据本发明的方法的溶液和试剂盒来代替。
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10.发明授权Process and a device for the isolation of cell components such as nucleic acids from natural sources 失效方法和用于分离细胞组分如来自天然来源的核酸的装置公开(公告)号：US06277648B1
公开(公告)日：2001-08-21
申请号：US08253152
申请日：1994-06-02
Applicant: Metin Colpan
Inventor： Metin Colpan
IPC: B01D3700
CPC classification number: C12N15/101 , B01D39/00 , C12N15/1017 , Y10T436/25375 , Y10T436/255
Abstract: Described is a method of isolating cell components, such as nucleic acids, from natural sources by filtering a sample of the digested natural sources such as cells or cell fragments. The method is characterized in that the sample is passed through a filter, the pore size of which decreases in the direction of flow of the sample through the filter.
Abstract translation: 描述了通过过滤消化的天然来源（例如细胞或细胞碎片）的样品从天然来源分离细胞成分（例如核酸）的方法。 该方法的特征在于，样品通过过滤器，其孔径在样品通过过滤器的流动方向上减小。
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