公开(公告)号：US10457958B2

公开(公告)日：2019-10-29

申请号：US16047187

申请日：2018-07-27

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas Cerruti ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Arlene R. Howe ,  Sara Ann Salvador

IPC: C12N15/82 ,  C07K14/325 ,  A01N47/08 ,  A01N63/02

Abstract: Engineered Cry1Da amino acid sequences are provided that exhibit improved Lepidopteran insecticidal activity and an enhanced Lepidopteran spectrum compared to the naturally occurring Cry1Da protein toxin. Polynucleotide sequences intended for use in expression of the improved proteins in plants are also provided. Particular embodiments provide compositions containing insect inhibitory amounts of the engineered proteins, as well as recombinant plants, plant parts, and seeds containing polynucleotide constructs encoding one or more of the improved engineered proteins.

公开(公告)号：US10017549B2

公开(公告)日：2018-07-10

申请号：US13950439

申请日：2013-07-25

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Stanislaw Flasinski ,  Gregory R. Heck ,  Stephen R. Penn ,  Uma Rao Sukuru ,  Xiaohong Shi

IPC: C07K14/325 ,  A01N63/00 ,  C12N15/82

CPC classification number: C07K14/325 ,  A01N63/00 ,  C12N15/8286 ,  Y02A40/162

Abstract: A novel Bacillus thuringiensis crystal protein exhibiting insect inhibitory activity is disclosed. Growth of Lygus insects is significantly inhibited by providing the novel crystal protein in Lygus insect diet. Polynucleotides encoding the crystal protein, transgenic plants and microorganisms that contain the polynucleotides, isolated peptides derived from the crystal protein, and antibodies directed against the crystal protein are also provided. Methods of using the crystal protein and polynucleotides encoding the crystal protein to control Hemipteran insects are also disclosed.

公开(公告)号：US20180127772A1

公开(公告)日：2018-05-10

申请号：US15848837

申请日：2017-12-20

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C12N15/82

CPC classification number: C07K14/325 ,  A01N63/02 ,  C07K2319/00 ,  C12N15/62 ,  C12N15/8286 ,  Y02A40/162

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

公开(公告)号：US20170114362A1

公开(公告)日：2017-04-27

申请号：US15348706

申请日：2016-11-10

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Claire A. CaJacob ,  Pascale Feldmann ,  Gregory R. Heck ,  Irene Maria Antonia Nooren ,  Geert Plaetinck ,  Wendy T. Maddelein ,  Ty T. Vaughn

IPC: C12N15/82 ,  C07K14/435

CPC classification number: C12N15/8286 ,  C07H21/04 ,  C07K14/43536 ,  C07K14/43563 ,  C12N15/113 ,  C12N15/8218 ,  C12N15/8282 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

Abstract: The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control, By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US09340797B2

公开(公告)日：2016-05-17

申请号：US13783125

申请日：2013-03-01

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Larry A. Gilbertson ,  David K. Kovalic ,  Thomas J. LaRosa ,  Maolong Lu ,  Tichafa R. I. Munyikwa ,  James K. Roberts ,  Wei Wu ,  Bei Zhang

IPC: C12N15/82 ,  C12N15/87 ,  A01H1/00 ,  A01N63/02

CPC classification number: C12N15/8286 ,  A01N63/02 ,  C12N15/8275 ,  C12N15/8279 ,  C12N15/8285 ,  Y02A40/162 ,  Y02A40/164

Abstract: The present invention is directed to controlling pest infestation by inhibiting one or more biological functions in an invertebrate pest. The invention discloses methods and compositions for use in controlling pest infestation by feeding one or more different recombinant double stranded RNA molecules to the pest in order to achieve a reduction in pest infestation through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US20240309057A1

公开(公告)日：2024-09-19

申请号：US18633982

申请日：2024-04-12

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Thomas A. Cerruti ,  Crystal L. Dart ,  Leigh H. English ,  Stanislaw Flasinski ,  Xiaoran Fu ,  Victor M. Guzov ,  Arlene R. Howe ,  Jay P. Morgenstern ,  James K. Roberts ,  Sara A. Salvador ,  Jinling Wang

IPC: C07K14/325 ,  A01N37/46 ,  C12N15/62 ,  C12N15/82

CPC classification number: C07K14/325 ,  A01N37/46 ,  C12N15/8286 ,  C07K2319/00 ,  C12N15/62 ,  Y02A40/146

Abstract: Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

公开(公告)号：US11981709B2

公开(公告)日：2024-05-14

申请号：US17742215

申请日：2022-05-11

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Artem G. Evdokimov ,  Farhad Moshiri ,  Timothy J. Rydel ,  Eric J. Sturman ,  Moritz von Rechenberg ,  Halong Vu ,  Andrew M. Wollacott ,  Meiying Zheng ,  Stanislaw Flasinski

IPC: C12N15/62 ,  A01N37/46 ,  C07K14/00 ,  C07K14/32 ,  C07K14/325 ,  C07K14/435 ,  C12N15/82

CPC classification number: C07K14/325 ,  A01N37/46 ,  C07K14/001 ,  C07K14/32 ,  C07K14/43577 ,  C12N15/8286 ,  Y02A40/146

Abstract: The present invent in discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

公开(公告)号：US20220356215A1

公开(公告)日：2022-11-10

申请号：US17742219

申请日：2022-05-11

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： James A. Baum ,  Artem G. EVDOKIMOV ,  Farhad MOSHIRI ,  Timothy J. RYDEL ,  Eric J. STURMAN ,  Moritz von RECHENBERG ,  Halong VU ,  Andrew M. WOLLACOTT ,  Meiying ZHENG

IPC: C07K14/325 ,  C12N15/82 ,  C07K14/00 ,  A01N37/46 ,  C07K14/435 ,  C07K14/32

Abstract: The present invention discloses Hemipteran insect inhibitory proteins, methods of using such proteins, nucleotide sequences encoding such proteins, methods of detecting and isolating such proteins, and their use in agricultural systems.

公开(公告)号：US11312975B2

公开(公告)日：2022-04-26

申请号：US16706285

申请日：2019-12-06

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Claire A. CaJacob ,  Pascale Feldman ,  Gregory R. Heck ,  Irene Maria Antonia Nooren ,  Geert Plaetinck ,  Wendy T. Maddelein ,  Ty T. Vaughn

IPC: C12N15/82 ,  C07H21/04 ,  C07K14/435 ,  C12N15/113

Abstract: The present invention relates to control of pest infestation by inhibiting one or more biological functions. The invention provides methods and compositions for such control, By feeding one or more recombinant double stranded RNA molecules provided by the invention to the pest, a reduction in pest infestation is obtained through suppression of gene expression. The invention is also directed to methods for making transgenic plants that express the double stranded RNA molecules, and to particular combinations of transgenic pesticidal agents for use in protecting plants from pest infestation.

公开(公告)号：US20220081695A1

公开(公告)日：2022-03-17

申请号：US17401851

申请日：2021-08-13

Applicant: Monsanto Technology LLC

Inventor： James A. Baum ,  Brian D. Eads ,  Jeremy A. Kroemer ,  Christina Marie Taylor

IPC: C12N15/82 ,  A01N63/60 ,  A01N63/10 ,  A01N57/16

Abstract: Disclosed herein are methods of controlling insect pests which infest crop plants, in particular Spodoptera frugiperda (fall armyworm), Lygus hesperus (western tarnished plant bug), Euschistus heros (neotropical brown stink bug), and Plutella xylostella (diamondback moth), and methods of providing plants resistant to such pests. Also disclosed are polynucleotides and recombinant DNA molecules and constructs useful in such methods, insecticidal compositions such as topical sprays containing insecticidal double-stranded RNAs, and plants with improved resistance to infestation by these insects. Further disclosed are methods of selecting target genes for RNAi-mediated silencing and control of these insect pests.