公开(公告)号：US10240165B2

公开(公告)日：2019-03-26

申请号：US15376495

申请日：2016-12-12

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Susan J. Johnson ,  Michael W. Petersen ,  David Walters ,  Paul S. Chomet

IPC: C12N15/82 ,  C12N15/84 ,  C12N9/10

Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

公开(公告)号：US20180023090A1

公开(公告)日：2018-01-25

申请号：US15638200

申请日：2017-06-29

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N5/04 ,  C12N9/10 ,  G01N33/00 ,  A01H4/00

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20170253937A1

公开(公告)日：2017-09-07

申请号：US15376495

申请日：2016-12-12

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Larry A. Gilbertson ,  Shihshieh Huang ,  Susan J. Johnson ,  Michael W. Petersen ,  David Walters ,  Paul S. Chomet

IPC: C12Q1/68 ,  A01H1/04 ,  C12N9/10 ,  C12N15/82

CPC classification number: C12N15/8265 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/821 ,  C12N15/8212 ,  C12N15/8218 ,  C12N15/8222 ,  C12N15/8231 ,  C12N15/8234 ,  C12N15/8261 ,  C12N15/8263 ,  C12N15/8267 ,  C12N15/8287 ,  C12N15/8289 ,  C12N15/829 ,  C12N15/8294

Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.

公开(公告)号：US09714428B2

公开(公告)日：2017-07-25

申请号：US14507744

申请日：2014-10-06

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  A01H1/04 ,  A01H4/00 ,  C12N5/04 ,  G01N33/00 ,  C12N9/10

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20160319289A1

公开(公告)日：2016-11-03

申请号：US15151383

申请日：2016-05-10

Applicant: MONSANTO TECHNOLOGY LLC

Inventor： Xudong Ye ,  Junjiang Shen ,  Edward Williams

IPC: C12N15/82 ,  G01N33/50 ,  C12R1/41

CPC classification number: C12N15/8202 ,  C12N15/8205 ,  C12R1/41 ,  G01N33/5097

Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.

公开(公告)号：US20150113683A1

公开(公告)日：2015-04-23

申请号：US14507744

申请日：2014-10-06

Applicant: Monsanto Technology LLC

Inventor： Brian J. Martinell ,  Michael W. Petersen ,  David A. Somers ,  Yuechun Wan ,  Edward Williams ,  Xudong Ye

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8201 ,  A01H4/003 ,  A01H4/008 ,  C12N5/04 ,  C12N9/1029 ,  C12N15/8202 ,  C12N15/8205 ,  C12N15/8209 ,  C12N15/8271 ,  C12N15/8274 ,  C12N15/8275 ,  C12N15/8277 ,  C12N15/8281 ,  C12Y203/01081 ,  G01N33/0098

Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.

公开(公告)号：US20150013031A1

公开(公告)日：2015-01-08

申请号：US14295096

申请日：2014-06-03

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

Abstract translation: 本发明涉及通过使用与重组构建体上的T-DNA边界序列有效连接的其它转化增强子序列（例如过度驱动或TSS序列）来提高农杆菌和根瘤土壤介导的植物细胞转化效率的方法和组合物 其包含T-DNA。

公开(公告)号：US08759616B2

公开(公告)日：2014-06-24

申请号：US13847127

申请日：2013-03-19

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/84 ,  C12N5/04 ,  C12N5/10 ,  C12N1/21 ,  A01H5/00

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

Abstract translation: 本发明涉及通过使用与重组构建体上的T-DNA边界序列有效连接的其它转化增强子序列（例如过度驱动或TSS序列）来提高农杆菌和根瘤土壤介导的植物细胞转化效率的方法和组合物 其包含T-DNA。

公开(公告)号：US20130219564A1

公开(公告)日：2013-08-22

申请号：US13847127

申请日：2013-03-19

Applicant: Monsanto Technology LLC

Inventor： Larry A. Gilbertson ,  Xudong Ye

IPC: C12N15/82

CPC classification number: C12N15/8205 ,  C12N15/8202

Abstract: The present invention relates to methods and compositions for improving the efficiency of Agrobacterium- and Rhizobium-mediated plant cell transformation by use of additional transformation enhancer sequences, such as overdrive or TSS sequences, operably linked to a T-DNA border sequence on a recombinant construct that comprises T-DNA.

公开(公告)号：US20130217008A1

公开(公告)日：2013-08-22

申请号：US13847928

申请日：2013-03-20

Applicant: Monsanto Technology LLC

Inventor： Xudong Ye

IPC: C12N15/67 ,  C12Q1/68

CPC classification number: C12N15/67 ,  C12N15/743 ,  C12Q1/68

Abstract: The present invention provides regulatory polynucleotide molecules isolated from a 16S rDNA for enhanced expression of heterologous genes. The invention further discloses compositions, polynucleotide constructs, transformed host cells containing the regulatory polynucleotide sequences, and methods for preparing and using the same.