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公开(公告)号：US11629357B2
公开(公告)日：2023-04-18
申请号：US17079348
申请日：2020-10-23
Applicant: Monsanto Technology LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Shihshieh Huang , Susan J. Johnson , Michael W. Petersen , David Walters , Paul S. Chomet
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
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公开(公告)号：US10808257B2
公开(公告)日：2020-10-20
申请号：US15843823
申请日：2017-12-15
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Larry A. Gilbertson , Shihshieh Huang , Thomas Malvar
IPC: C12N15/82
Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
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公开(公告)号：US20180263205A1
公开(公告)日：2018-09-20
申请号：US15917489
申请日：2018-03-09
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Michael H. Luethy , Thomas M. Malvar , Shihshieh Huang
Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
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公开(公告)号：US09913487B2
公开(公告)日：2018-03-13
申请号：US13914505
申请日：2013-06-10
Applicant: Monsanto Technology LLC
Inventor： Michael H. Luethy , Thomas M. Malvar , Shihshieh Huang
CPC classification number: A01H5/10 , A23K10/30 , A23L7/198 , C07K14/415 , C12N9/0028 , C12N15/8218 , C12N15/8247 , C12N15/8251 , C12N15/8254
Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
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公开(公告)号：US09192112B2
公开(公告)日：2015-11-24
申请号：US13677607
申请日：2012-11-15
Applicant: Monsanto Technology LLC
Inventor： Edwards Allen , Larry A Gilbertson , James K Roberts , Nancy M Houmard , Shihshieh Huang , Sergey Ivashuta
IPC: A01H1/02 , C12N15/82 , C12N15/113 , A01H5/00 , A01H5/10
CPC classification number: C12N15/8218 , A01H1/02 , A01H5/00 , A01H5/10 , C12N15/8287 , C12N15/8289 , C12N15/829
Abstract: This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites.
Abstract translation: 本发明提供了生产非天然杂种种子的方法。 还公开了可用于在作物植物上赋予诱导型不育性的特异性miRNA和miRNA识别位点，以及包含这种外源miRNA识别位点的重组DNA构建体。
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Patent Agency Ranking
公开(公告)号：US20150166993A1
公开(公告)日：2015-06-18
申请号：US14567786
申请日：2014-12-11
Applicant: Monsanto Technology LLC
Inventor： Edwards M. Allen , Barry S. Goldman , Liang Guo , Sara E. Heisel , Shihshieh Huang , Sergey I. Ivashuta , David K. Kovalic , Elysia K. Krieger , James K. Roberts , Yuanji Zhang
IPC: C12N15/113 , C12N15/82
CPC classification number: C12N15/113 , C12N15/8216 , C12N15/8218 , C12N15/8238 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N2310/141 , C12N2320/50 , Y02A40/146 , Y02A40/162
Abstract: This invention discloses novel microRNAs and their precursors, and recombinant DNA constructs including such novel miRNAs, miRNA precursors, miRNA promoters, and miRNA recognition sites corresponding to the miRNAs. Included are novel miRNA and miRNA precursors that exhibit nutrient-responsive expression. Also disclosed are miRNA decoy sequences. Further provided are non-natural transgenic plant cells, plants, and seeds containing in their genome a recombinant DNA construct of this invention and methods of controlling gene expression using recombinant DNA constructs of this invention.
Abstract translation: 本发明公开了新的微小RNA及其前体，以及重组DNA构建体，包括这样的新型miRNA，miRNA前体，miRNA启动子和对应于miRNA的miRNA识别位点。 包括显示营养反应表达的新型miRNA和miRNA前体。 还公开了miRNA诱饵序列。 还提供了非天然转基因植物细胞，植物和种子，在其基因组中含有本发明的重组DNA构建体和使用本发明的重组DNA构建体控制基因表达的方法。
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公开(公告)号：US10772276B2
公开(公告)日：2020-09-15
申请号：US15917489
申请日：2018-03-09
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Michael H. Luethy , Thomas M. Malvar , Shihshieh Huang
Abstract: Anti-sense-oriented RNA gene suppression agents in the form of a loop of anti-sense-oriented RNA is produced in cells of transgenic organisms, e.g. plants, by transcription from a recombinant DNA construct that comprises in 5′ to 3′ order a promoter element operably linked to more than one anti-sense-oriented DNA element and one or more complementary DNA elements.
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公开(公告)号：US20190249189A1
公开(公告)日：2019-08-15
申请号：US16279798
申请日：2019-02-19
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Shihshieh Huang , Stanislaw Flasinski , Alessandra Frizzi , Brad Gabor , Charles Hagen , John Kao , Raquel Salati
IPC: C12N15/82
CPC classification number: C12N15/8283 , C12N15/8218
Abstract: The present invention provides gene targets, constructs and methods for the genetic control of plant disease caused by multiple plant viruses. The present invention relates to achieving a plant protective effect through the identification of target coding sequences and the use of recombinant DNA technologies for post-transcriptionally repressing or inhibiting expression of the target coding sequences of plant-parasitic viruses. Protein-expression based approaches may also be utilized to augment phenotype resistance. Thus, transcription of a single transgenic event comprising one or more plant expression cassettes can allow for broad spectrum resistance of a plant to multiple plant viral strains and species among the geminiviruses, tospoviruses, and potexviruses.
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公开(公告)号：US10240165B2
公开(公告)日：2019-03-26
申请号：US15376495
申请日：2016-12-12
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Xudong Ye , Larry A. Gilbertson , Shihshieh Huang , Susan J. Johnson , Michael W. Petersen , David Walters , Paul S. Chomet
Abstract: The invention provides methods and compositions for identifying transgenic seed that contain a transgene of interest, but lack a marker gene. Use of an identification sequence that results in a detectable phenotype increases the efficiency of screening for seed and plants in which transgene sequences not linked to a gene of interest have segregated from the sequence encoding a gene of interest.
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公开(公告)号：US09856485B2
公开(公告)日：2018-01-02
申请号：US14337932
申请日：2014-07-22
Applicant: Monsanto Technology LLC
Inventor： Larry A. Gilbertson , Shihshieh Huang , Thomas Malvar
CPC classification number: C12N15/8213 , C12N15/63 , C12N15/8217 , C12N15/8218 , C12N15/8243
Abstract: Recombinant DNA constructs, for use in plants and plant cells, have site-specific recombination sites that allow assessing phenotypes and modes of action by over expression or suppression of endogenous genes. In an aspect, a single DNA construct can be switched between over expression and suppression by the action of a recombinase such as the Cre recombinase on constructs having lox recombination sites. Other useful recombination systems include the Flp/frt system, the R/Rs system, the Dre/rox system, and the GIN/gix system.
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