公开(公告)号：US11001869B2

公开(公告)日：2021-05-11

申请号：US16995820

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20200377921A1

公开(公告)日：2020-12-03

申请号：US16995820

申请日：2020-08-18

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20190093143A1

公开(公告)日：2019-03-28

申请号：US16211632

申请日：2018-12-06

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12P21/02 ,  C12N15/80 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20150225761A1

公开(公告)日：2015-08-13

申请号：US14663118

申请日：2015-03-19

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80

CPC classification number: C12P21/00 ,  C12N9/58 ,  C12N15/80 ,  C12P21/02

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

Abstract translation: 本发明涉及母体木霉菌株的突变体，其包含编码多肽的多核苷酸和选自第一枯草杆菌蛋白酶样丝氨酸蛋白酶基因，第一天冬氨酸蛋白酶基因，胰蛋白酶抑制蛋白酶基因的一个或多个（若干）基因， 如丝氨酸蛋白酶基因，第二枯草杆菌蛋白酶样丝氨酸蛋白酶基因和第二天冬氨酸蛋白酶基因，其中所述一个或多个（若干）基因被修饰，使得突变菌株缺乏产生一种或多种（若干）选自 与母本木霉属菌株相比，分别在相同的条件下培养时由第一枯草杆菌蛋白酶样丝氨酸蛋白酶，第一天冬氨酸蛋白酶，胰蛋白酶样丝氨酸蛋白酶，第二枯草杆菌蛋白酶样丝氨酸蛋白酶和第二天冬氨酸蛋白酶组成的组 条件。 本发明还涉及在这种突变体中产生多肽的方法和用于产生这种突变体的方法。

公开(公告)号：US20210155967A1

公开(公告)日：2021-05-27

申请号：US17163033

申请日：2021-01-29

Applicant: Novozymes, Inc.

Inventor： Suchindra Maiyuran ,  Abigail Jang ,  Kimberly Brown ,  Sandra Merino ,  Jeffrey Shasky ,  Eric Abbate

IPC: C12P21/00 ,  C12N15/80 ,  C12P21/02 ,  C12N9/58

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more (several) genes selected from the group consisting of a first subtilisin-like serine protease gene, a first aspartic protease gene, a trypsin-like serine protease gene, a second subtilisin-like serine protease gene, and a second aspartic protease gene, wherein the one or more (several) genes are modified rendering the mutant strain deficient in the production of one or more (several) enzymes selected from the group consisting of a first subtilisin-like serine protease, a first aspartic protease, a trypsin-like serine protease, a second subtilisin-like serine protease, and a second aspartic protease, respectively, compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US10023872B2

公开(公告)日：2018-07-17

申请号：US14652755

申请日：2013-12-20

Applicant: Novozymes, Inc.

Inventor： Sandra Merino ,  Judilee Quintos ,  Debbie Yaver

IPC: C12N15/00 ,  C12P21/00 ,  C12N15/52 ,  C12P21/02 ,  C12N9/88 ,  C12N9/00

Abstract: The present invention relates to mutants of a parent Trichoderma strain, comprising a polynucleotide encoding a polypeptide and one or more genes selected from the group consisting of a peptaibol synthetase gene, a paracelsin synthetase gene, a first terpene cyclase gene, a second terpene cyclase gene, and a third terpene cyclase gene, wherein one or more of the genes are modified rendering the mutant strain deficient in the production of one or more of the enzymes selected from the group consisting of a peptaibol synthetase, a paracelsin synthetase, a first terpene cyclase, a second terpene cyclase, and a third terpene cyclase compared to the parent Trichoderma strain when cultivated under identical conditions. The present invention also relates to methods of producing a polypeptide in such mutants and methods for producing such mutants.

公开(公告)号：US20160152993A1

公开(公告)日：2016-06-02

申请号：US15014764

申请日：2016-02-03

Applicant: Novozymes, Inc.

Inventor： Sandra Merino ,  Debbie Yaver

IPC: C12N15/80

CPC classification number: C12N15/80 ,  C12N9/0065 ,  C12P21/00

Abstract: The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.

公开(公告)号：US09006409B2

公开(公告)日：2015-04-14

申请号：US14524793

申请日：2014-10-27

Applicant: Novozymes, Inc.

Inventor： Sandra Merino

IPC: C07H21/04 ,  C12N1/00 ,  C12N15/00 ,  C12N9/42 ,  C12N15/80

CPC classification number: C12N9/2437 ,  C07K1/00 ,  C07K2319/00 ,  C07K2319/036 ,  C12N9/2434 ,  C12N9/2445 ,  C12N15/80 ,  C12P19/02 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01004

Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

公开(公告)号：US20150044756A1

公开(公告)日：2015-02-12

申请号：US14524793

申请日：2014-10-27

Applicant: NOVOZYMES, INC.

Inventor： Sandra Merino

IPC: C12N9/42 ,  C12N15/80

CPC classification number: C12N9/2437 ,  C07K1/00 ,  C07K2319/00 ,  C07K2319/036 ,  C12N9/2434 ,  C12N9/2445 ,  C12N15/80 ,  C12P19/02 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01004

Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

公开(公告)号：US20140248671A1

公开(公告)日：2014-09-04

申请号：US14279624

申请日：2014-05-16

Applicant: Novozymes, Inc.

Inventor： Sandra Merino

IPC: C12P19/14 ,  C12P19/02

CPC classification number: C12N9/2437 ,  C07K1/00 ,  C07K2319/00 ,  C07K2319/036 ,  C12N9/2434 ,  C12N9/2445 ,  C12N15/80 ,  C12P19/02 ,  C12P19/14 ,  C12P21/02 ,  C12Y302/01004

Abstract: The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium.

Abstract translation: 本发明涉及生产具有生物活性的分泌多肽的方法，其包括：（a）用编码融合蛋白的融合蛋白构建体转化真菌宿主细胞，其包含：（i）编码信号肽的第一多核苷酸; （ii）至少编码内切葡聚糖酶或其部分的催化结构域的第二多核苷酸; 和（iii）至少编码具有生物活性的多肽的催化结构域的第三个多核苷酸; 其中所述信号肽和至少所述内切葡聚糖酶的催化结构域与至少所述内切葡聚糖酶的催化结构域相比，增加具有生物活性的多肽的分泌; （b）在适合于产生融合蛋白的条件下培养转化的真菌宿主细胞; 和（c）从培养基中回收具有生物活性的融合蛋白，其组分或其组合。