公开(公告)号：US11492593B2

公开(公告)日：2022-11-08

申请号：US15028158

申请日：2014-10-09

Applicant: HEALIOS K.K. ,  OSAKA UNIVERSITY

Inventor： Masanori Sawada ,  Kiyotoshi Sekiguchi

IPC: C12N5/079 ,  A61K35/30 ,  A61K9/00

Abstract: The present invention provides a method of purifying highly pure retinal pigment epithelial cells from a cell population obtained by induction of differentiation of pluripotent stem cells into retinal pigment epithelial cells, by a simple and easy operation in a short period. The purification method of the present invention includes a step of introducing a cell population containing retinal pigment epithelial cells obtained by differentiation induction of pluripotent stem cells on laminin or a fragment thereof on a filter, and obtaining a cell population that passed the filter.

公开(公告)号：US11060061B2

公开(公告)日：2021-07-13

申请号：US16626052

申请日：2018-08-07

Applicant: MANDOM CORPORATION ,  OSAKA UNIVERSITY

Inventor： Tomohisa Hayakawa ,  Ryuichiro Kurata ,  Fumitaka Fujita ,  Fumihiro Okada ,  Kiyotoshi Sekiguchi

IPC: C12N5/077 ,  C12N15/85 ,  C12N15/86

Abstract: An immortalized sweat gland myoepithelial cell which expresses α-SMA and pan-cytokeratin and has a sphere forming ability after subculture at least 5 times. A method for producing immortalized sweat gland myoepithelial said method comprising: while culturing a cell structure, wherein sweat gland myoepithelial cells are exposed on a surface, in a state of being suspended in a medium, transferring an immortalizing gene into the cells; and then culturing the transgenic structure thus obtained in a state of being suspended in the medium to thereby obtain immortalized sweat gland myoepithelial cells.

公开(公告)号：US10696948B2

公开(公告)日：2020-06-30

申请号：US15511275

申请日：2015-09-14

Applicant: OSAKA UNIVERSITY

Inventor： Yukiko Ochi ,  Kiyotoshi Sekiguchi ,  Shigeru Miyagawa ,  Yoshiki Sawa ,  Antti Markus Siltanen

IPC: C12N5/077 ,  A61K35/34 ,  C12N5/074

Abstract: The present invention provides a method for preparing a clinically applicable, safe and less damaged cardiomyocyte population through a brief and simple procedure from a cell population obtained by induced differentiation of pluripotent stem cells into cardiomyocytes. The present invention relates to a method for preparing a cardiomyocyte population, the method comprising the steps of: (1) inducing pluripotent stem cells to differentiate into cardiomyocytes, (2) bringing a cell population obtained by the induced differentiation into contact with a laminin selected from the group consisting of laminin α2β1γ1, laminin α2β2γ1, laminin α1β1γ1 and laminin α1β2γ1, or a fragment thereof having integrin binding activity, and (3) retrieving cells adherent to the laminin or the laminin fragment.

公开(公告)号：US20230114089A1

公开(公告)日：2023-04-13

申请号：US17824603

申请日：2022-05-25

Applicant: KYOTO UNIVERSITY ,  OSAKA UNIVERSITY ,  EISAI R&D MANAGEMENT CO., LTD.

Inventor： Jun Takahashi ,  Daisuke Doi ,  Bumpei Samata ,  Kiyotoshi Sekiguchi ,  Yuichi Ono

IPC: C12N5/0797 ,  A61K35/30 ,  C12N5/0793

Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.

公开(公告)号：US11473058B2

公开(公告)日：2022-10-18

申请号：US14916696

申请日：2014-09-04

Applicant: KYOTO UNIVERSITY ,  OSAKA UNIVERSITY ,  EISAI R&D MANAGEMENT CO., LTD.

Inventor： Jun Takahashi ,  Daisuke Doi ,  Bumpei Samata ,  Kiyotoshi Sekiguchi ,  Yuichi Ono

IPC: A61K35/30 ,  C12N5/0797 ,  C12N5/0793

Abstract: The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.

公开(公告)号：US20190211305A1

公开(公告)日：2019-07-11

申请号：US16326841

申请日：2017-08-25

Applicant: Kyoto University ,  Osaka University ,  Megakaryon Corporation

Inventor： Koji Eto ,  Sou Nakamura ,  Kiyotoshi Sekiguchi ,  Tomohiro Shigemori

IPC: C12N5/0735 ,  C12N5/077 ,  C12N5/071 ,  C12N5/078

CPC classification number: C12N5/0606 ,  C12N5/00 ,  C12N5/0634 ,  C12N5/0657 ,  C12N5/0658 ,  C12N5/0687 ,  C12N5/10 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/165 ,  C12N2533/52

Abstract: The present invention provides a method for culturing pluripotent stem cells, comprising the step of contacting pluripotent stem cells with laminin 421 or a fragment thereof, laminin 121 or a fragment thereof, or a combination thereof.

公开(公告)号：US20180208893A1

公开(公告)日：2018-07-26

申请号：US15745425

申请日：2016-07-14

Applicant: Kyoto University ,  Osaka University

Inventor： Tatsutoshi Nakahata ,  Megumu Saito ,  Akira Niwa ,  Ryo Ota ,  Kiyotoshi Sekiguchi

IPC: C12N5/071

Abstract: Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.

公开(公告)号：US10000554B2

公开(公告)日：2018-06-19

申请号：US14758061

申请日：2013-11-11

Applicant: OSAKA UNIVERSITY

Inventor： Kiyotoshi Sekiguchi ,  Shaoliang Li ,  Ryoko Sato

IPC: C07K16/18 ,  C12N15/62 ,  C07K14/78 ,  A61L27/22 ,  C12N5/074 ,  A61K38/00

CPC classification number: C07K14/78 ,  A61L27/227 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/21 ,  C12N5/0696 ,  Y02P20/582

Abstract: A modified laminin characterized in that a laminin or a heterotrimeric laminin fragment has a collagen binding molecule conjugated to at least one site selected from the α chain N-terminus, the β chain N-terminus and the γ chain N-terminus, and an extracellular-matrix material comprising the modified laminin, and collagen and/or gelatin serve as an alternative to Matrigel and are useful as an extracellular-matrix material for the formation of a safe three-dimensional tissue structure for regenerative medicine in humans.

公开(公告)号：US20150086557A1

公开(公告)日：2015-03-26

申请号：US14384206

申请日：2013-03-14

Applicant: OSAKA UNIVERSITY

Inventor： Kiyotoshi Sekiguchi ,  Ryoko Sato ,  Sachiko Ezoe

IPC: C07K7/06 ,  C12N5/071 ,  G01N33/50 ,  C07K16/18 ,  C07K7/08

CPC classification number: C07K7/06 ,  A61K38/00 ,  C07K7/08 ,  C07K14/4702 ,  C07K14/7055 ,  C07K16/18 ,  C07K16/28 ,  C07K16/2839 ,  C07K2317/34 ,  C12N5/0602 ,  G01N33/502 ,  G01N2500/02 ,  G01N2500/04

Abstract: Provided is a novel ligand for integrin α9β1 consisting of a peptide having the following amino acid sequence: (A) EDDMMEVPY (SEQ ID NO: 1) or (B) an amino acid sequence the same as the amino acid sequence represented by SEQ ID NO: 1 except for having deletion, substitution or addition of 1 to 3 amino acids. The novel ligand for integrin α9β1 has a higher binding affinity than those of tenascin-C and osteodontin, which are known ligands for integrin α9β1.

Abstract translation: 提供一种由具有以下氨基酸序列的肽组成的整合素α9和bgr的新型配体：（A）EDDMMEVPY（SEQ ID NO：1）或（B）与SEQ ID NO：1所示的氨基酸序列相同的氨基酸序列 ID NO：1，除了缺失，取代或添加1至3个氨基酸。 整合素α9和bgr1的新型配体具有比蜕皮素C和骨质疏松的更高的结合亲和力，其是用于整合素α9和bgr的已知配体1。