-
公开(公告)号：US11427806B2
公开(公告)日：2022-08-30
申请号：US14916696
申请日：2014-09-04
发明人： Jun Takahashi , Daisuke Doi , Bumpei Samata , Kiyotoshi Sekiguchi , Yuichi Ono
IPC分类号： A61K35/30 , C12N5/0797 , C12N5/0793
摘要： The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
-
公开(公告)号：US10669529B2
公开(公告)日：2020-06-02
申请号：US15745425
申请日：2016-07-14
申请人： Kyoto University , Osaka University
发明人： Tatsutoshi Nakahata , Megumu Saito , Akira Niwa , Ryo Ota , Kiyotoshi Sekiguchi
摘要： Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.
-
公开(公告)号：US11959103B2
公开(公告)日：2024-04-16
申请号：US16348944
申请日：2017-11-09
申请人： OSAKA UNIVERSITY , KYOTO UNIVERSITY
IPC分类号： C12N5/0735 , A61K35/34 , A61K35/545 , A61P9/00 , A61P21/00 , A61P43/00 , C12N5/071 , C12N5/077 , C12N5/10
CPC分类号： C12N5/0657 , A61K35/34 , A61K35/545 , A61P9/00 , A61P21/00 , A61P43/00 , C12N5/0658 , C12N5/069 , C12N5/10 , C12N2501/115 , C12N2501/12 , C12N2501/155 , C12N2501/16 , C12N2501/165 , C12N2501/91 , C12N2501/998 , C12N2506/45
摘要： Provided is a method for inducing pluripotent stem cells to differentiate into somatic cells in a culture medium containing a heparin binding growth factor, the method comprising bringing cells into contact with a conjugate of a laminin E8 fragment and a growth factor binding domain-containing fragment of a heparan sulfate proteoglycan. According to the present invention, pluripotent stem cells can be induced to differentiate into any desired somatic cells in a highly efficient manner.
-
公开(公告)号：US20160215260A1
公开(公告)日：2016-07-28
申请号：US14916696
申请日：2014-09-04
发明人： Jun Takahashi , Daisuke Doi , Bumpei Samata , Kiyotoshi Sekiguchi , Yuichi Ono
IPC分类号： C12N5/0797 , A61K35/30
CPC分类号： C12N5/0623 , A61K35/30 , C12N5/0619 , C12N2501/119 , C12N2501/13 , C12N2501/15 , C12N2501/155 , C12N2501/41 , C12N2501/415 , C12N2501/727 , C12N2506/02 , C12N2506/03 , C12N2506/45 , C12N2533/52
摘要： The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
摘要翻译： 本发明提供了一种用于从多能干细胞产生多巴胺能神经元祖细胞的方法，该方法包括以下步骤：（i）在含有选自以下的试剂的培养基中，在细胞外基质上进行多能干细胞的贴壁培养： 由BMP抑制剂，TGFβ抑制剂，SHH信号刺激剂，FGF8和GSK3β抑制剂组成的组; （ii）使用与Corin结合的物质和/或与Lrtm1结合的物质从步骤（i）中获得的细胞收集Corin和/或Lrtm1阳性细胞; 和（iii）在含有神经营养因子的培养基中进行步骤（ii）中获得的细胞的悬浮培养。
-
公开(公告)号：US20230114089A1
公开(公告)日：2023-04-13
申请号：US17824603
申请日：2022-05-25
发明人： Jun Takahashi , Daisuke Doi , Bumpei Samata , Kiyotoshi Sekiguchi , Yuichi Ono
IPC分类号： C12N5/0797 , A61K35/30 , C12N5/0793
摘要： The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
-
公开(公告)号：US11473058B2
公开(公告)日：2022-10-18
申请号：US14916696
申请日：2014-09-04
发明人： Jun Takahashi , Daisuke Doi , Bumpei Samata , Kiyotoshi Sekiguchi , Yuichi Ono
IPC分类号： A61K35/30 , C12N5/0797 , C12N5/0793
摘要： The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
-
公开(公告)号：US20190211305A1
公开(公告)日：2019-07-11
申请号：US16326841
申请日：2017-08-25
IPC分类号： C12N5/0735 , C12N5/077 , C12N5/071 , C12N5/078
CPC分类号： C12N5/0606 , C12N5/00 , C12N5/0634 , C12N5/0657 , C12N5/0658 , C12N5/0687 , C12N5/10 , C12N2501/115 , C12N2501/15 , C12N2501/165 , C12N2533/52
摘要： The present invention provides a method for culturing pluripotent stem cells, comprising the step of contacting pluripotent stem cells with laminin 421 or a fragment thereof, laminin 121 or a fragment thereof, or a combination thereof.
-
公开(公告)号：US20180208893A1
公开(公告)日：2018-07-26
申请号：US15745425
申请日：2016-07-14
申请人： Kyoto University , Osaka University
发明人： Tatsutoshi Nakahata , Megumu Saito , Akira Niwa , Ryo Ota , Kiyotoshi Sekiguchi
IPC分类号： C12N5/071
摘要： Provided is a method for producing vascular endothelial cells from pluripotent stem cells, the method comprising the following steps (i) to (iii): (i) a step of culturing pluripotent stem cells in a culture medium comprising a BMP, on a culture vessel coated with a first matrix, to produce mesodermal progenitor cells; (ii) a step of dissociating the resulting cells into single cells; and (iii) a step of culturing the resulting cells in a culture medium comprising VEGF, on a culture vessel coated with a second matrix selected from the group consisting of laminin-411 or a fragment thereof, laminin-511 or a fragment thereof, Matrigel, type IV collagen and fibronectin.
-
公开(公告)号：US10428311B2
公开(公告)日：2019-10-01
申请号：US15325743
申请日：2015-07-15
申请人： OSAKA UNIVERSITY
发明人： Kiyotoshi Sekiguchi , Ko Tsutsui
摘要： The present invention provides a novel technique in a cell culture method using a cell culture vessel coated with a laminin fragment, which novel technique achieves cell culture as in the case of using a recommended coating concentration even when the coating concentration is lower than the recommended coating concentration. The present invention relates to a method for enhancing an activity for mammalian cultured cells of a laminin fragment or a variant thereof each having integrin binding activity.
-
公开(公告)号：US11649433B2
公开(公告)日：2023-05-16
申请号：US16482160
申请日：2018-01-31
申请人： Osaka University
IPC分类号： A61K35/545 , C12N5/079 , A61K38/18
CPC分类号： C12N5/0621 , A61K35/545 , A61K38/1825 , A61K38/18 , C12N2501/998 , C12N2506/45 , C12N2533/52
摘要： The present invention relates to a method for controlling differentiation of pluripotent stem cells, which method comprises selecting a laminin or a fragment thereof based on binding affinity for the pluripotent stem cells and inducing differentiation of the pluripotent stem cells in the presence of the laminin or a fragment thereof. Here, the binding affinity for cells can be assessed by time-course observation of the survival rate and motility of the cells. According to the present invention, a cell population containing any desired proportion of differentiated cells can be produced from pluripotent stem cells in a simple manner. The cell population obtained by this production method is very useful for cell therapy-based treatment strategies for diseases.
-
-
-
-
-
-
-
-
-
搜索结果
19 条记录 (耗时 0.022 秒)
