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公开(公告)号：US20190211305A1
公开(公告)日：2019-07-11
申请号：US16326841
申请日：2017-08-25
IPC分类号： C12N5/0735 , C12N5/077 , C12N5/071 , C12N5/078
CPC分类号： C12N5/0606 , C12N5/00 , C12N5/0634 , C12N5/0657 , C12N5/0658 , C12N5/0687 , C12N5/10 , C12N2501/115 , C12N2501/15 , C12N2501/165 , C12N2533/52
摘要： The present invention provides a method for culturing pluripotent stem cells, comprising the step of contacting pluripotent stem cells with laminin 421 or a fragment thereof, laminin 121 or a fragment thereof, or a combination thereof.
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2.发明申请公开(公告)号：US20210062158A1
公开(公告)日：2021-03-04
申请号：US16839906
申请日：2020-04-03
发明人： Koji Eto , Hiroshi Endo , Tomohiro Shigemori
摘要： Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).
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公开(公告)号：US20190032015A1
公开(公告)日：2019-01-31
申请号：US16071598
申请日：2017-01-30
发明人： Koji Eto , Hideya Seo , Akira Ota , Yukitaka Ito , Yasuko Hazama
IPC分类号： C12N5/078
摘要： The present invention provides: a platelet production promoter that contains one or more substances selected from the group consisting of Wnt inhibitors and FMS-like tyrosine kinase (FLT) inhibitors; and a platelet production method that uses this platelet production promoter.
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公开(公告)号：US11976301B2
公开(公告)日：2024-05-07
申请号：US17209035
申请日：2021-03-22
发明人： Takeaki Dohda , Sachiko Kobayashi , Koji Eto , Hiroshi Endo , Sou Nakamura
CPC分类号： C12N5/0644 , C12N2500/25 , C12N2500/38 , C12N2501/125 , C12N2501/145 , C12N2501/727 , C12N2501/998 , C12N2506/45 , C12N2510/00
摘要： The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.
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公开(公告)号：US11136547B2
公开(公告)日：2021-10-05
申请号：US16083503
申请日：2017-03-22
发明人： Koji Eto , Hiroshi Endo , Yukitaka Ito , Junichi Fukunaga
IPC分类号： A61K35/19 , A61K35/28 , A61K35/545 , C12N5/0735 , C12N5/078 , C12N5/00 , C12N5/10 , C12N15/09 , A61P7/08 , A61K38/18 , C12N5/074
摘要： Provided is a method for inducing mesoderm, comprising a step of bringing pluripotent stem cells into contact with bone morphogenetic protein 4 (BMP4) or CHIR for at least 3 days.
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公开(公告)号：US20210222124A1
公开(公告)日：2021-07-22
申请号：US17209035
申请日：2021-03-22
发明人： Takeaki Dohda , Sachiko Kobayashi , Koji Eto , Hiroshi Endo , Sou Nakamura
IPC分类号： C12N5/078
摘要： The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.
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公开(公告)号：US10941382B2
公开(公告)日：2021-03-09
申请号：US16071598
申请日：2017-01-30
发明人： Koji Eto , Hideya Seo , Akira Ota , Yukitaka Ito , Yasuko Hazama
IPC分类号： C12N5/078 , A61P7/04 , A61K31/444 , A61K31/381 , C12N15/09 , A61K35/19
摘要： The present invention provides: a platelet production promoter that contains one or more substances selected from the group consisting of Wnt inhibitors and FMS-like tyrosine kinase (FLT) inhibitors; and a platelet production method that uses this platelet production promoter.
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8.发明申请公开(公告)号：US20180051260A1
公开(公告)日：2018-02-22
申请号：US15565922
申请日：2016-04-14
发明人： Koji Eto , Hiroshi Endo , Tomohiro Shigemori
CPC分类号： C12N5/0696 , C12N5/0644 , C12N2506/11 , C12N2506/45 , C12N2510/00
摘要： Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).
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9.发明申请公开(公告)号：US20180044634A1
公开(公告)日：2018-02-15
申请号：US15555626
申请日：2016-03-09
发明人： Takeaki Dohda , Sachiko Kobayashi , Koji Eto , Hiroshi Endo , Sou Nakamura
IPC分类号： C12N5/078
CPC分类号： C12N5/0644 , C12N2500/25 , C12N2500/38 , C12N2501/125 , C12N2501/145 , C12N2501/727 , C12N2501/998 , C12N2506/45 , C12N2510/00
摘要： The present invention provides a method for producing megakaryocytes that have an increased capacity to produce platelets, the method comprising a step of culturing, under conditions that cause the death of cells that do not express a gene that is specifically expressed by megakaryocytes, cells that have the capacity to differentiate into megakaryocytes.
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10.发明公开公开(公告)号：US20240026304A1
公开(公告)日：2024-01-25
申请号：US18476065
申请日：2023-09-27
发明人： Koji Eto , Hiroshi Endo , Tomohiro Shigemori
CPC分类号： C12N5/0696 , C12N5/0644 , C12N2506/11 , C12N2506/45 , C12N2510/00
摘要： Provided is a method for producing a stem cell clone, which comprises the steps of: (i) introducing into stem cells an exogenous gene associated with induction of differentiation into somatic cells; (ii) inducing differentiation of the stem cells, introduced with an exogenous gene, into the somatic cells; (iii) dedifferentiating the differentiation-induced somatic cells; and (iv) isolating stem cells having the exogenous gene incorporated into a chromosome thereof from a colony of the stem cells formed in step (iii).
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