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公开(公告)号:US20230323310A1
公开(公告)日:2023-10-12
申请号:US18043507
申请日:2021-09-03
Applicant: TOPPAN INC. , OSAKA UNIVERSITY
Inventor: Tomoko KUNITOMI , Shiro KITANO , Kei TSUKAMOTO , Michiya MATSUSAKI
CPC classification number: C12N5/069 , C12N5/0656 , C12N2513/00 , C12N2533/54 , C12N2501/91
Abstract: The present invention relates to a method for producing a three-dimensional tissue body, including: a step of obtaining a mixture by mixing a cationic substance and a fragmented extracellular matrix component with cells; and a step of culturing the cells after the step of obtaining the mixture.
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公开(公告)号:US20220133952A1
公开(公告)日:2022-05-05
申请号:US17502496
申请日:2021-10-15
Applicant: TOPPAN PRINTING CO., LTD. , OSAKA UNIVERSITY
Inventor: Michiya MATSUSAKI , Shinji IRIE , Shiro KITANO
Abstract: A method may comprises bringing cells suspended in an aqueous medium into contact with a plurality of fragmented collagen pieces and, after the cells brought into contact with the plurality of fragmented collagen pieces and the plurality of fragmented collagen pieces are concentrated, culturing the cells brought into contact with the fragmented collagen pieces, with the plurality of fragmented collagen pieces, to form a three-dimensional tissue.
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13.
公开(公告)号:US20190351098A1
公开(公告)日:2019-11-21
申请号:US16480114
申请日:2018-01-31
Applicant: TOPPAN PRINTING CO., LTD. , OSAKA UNIVERSITY
Inventor: Michiya MATSUSAKI , Shinji IRIE , Shiro KITANO
Abstract: Disclosed is a three-dimensional tissue comprising cells and collagen including endogenous collagen, wherein at least a portion of the cells is adhered to the collagen, and the content of the collagen is from 10 wt % to 90 wt % based on the three-dimensional tissue.
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公开(公告)号:US20250057974A1
公开(公告)日:2025-02-20
申请号:US18723980
申请日:2022-12-14
Applicant: TOPPAN Holdings Inc. , OSAKA UNIVERSITY
Inventor: Michiya MATSUSAKI , Masahiko NAKAMOTO , Shiro KITANO
Abstract: The present invention relates to a method for changing a hydrogel volume, the method including a step A of bringing an ionic polymer into contact with a hydrogel, which has a site having decomposition activity for the ionic polymer and a cross-linkable functional group that is capable of cross-linking with the ionic polymer through electrostatic interaction, to allow the cross-linkable functional group to cross-link with the ionic polymer, and reducing a volume of the hydrogel; and a step B of decomposing the ionic polymer cross-linking with the hydrogel by using the site having decomposition activity and discharging at least part of a decomposition product of the ionic polymer from the hydrogel to increase the hydrogel volume.
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公开(公告)号:US20240352198A1
公开(公告)日:2024-10-24
申请号:US18686227
申请日:2022-08-22
Applicant: OSAKA UNIVERSITY , TOPPAN HOLDINGS INC.
Inventor: Michiya MATSUSAKI , Donghee KANG
Abstract: Provided are a method for producing a protein material without using an organic solvent, the method involving ejecting a solution containing a protein material raw material into a water-based medium and drying the ejected protein material raw material, and a protein material produced by the method.
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公开(公告)号:US20180355322A1
公开(公告)日:2018-12-13
申请号:US16104533
申请日:2018-08-17
Applicant: TOPPAN PRINTING CO., LTD. , OSAKA UNIVERSITY
Inventor: Kei TSUKAMOTO , Shinji IRIE , Michiya MATSUSAKI
IPC: C12N5/09
CPC classification number: C12N5/0693 , C12M3/00 , C12N2501/91
Abstract: A method of promoting spheroid formation, including: a preparation step of preparing a mixture obtained by mixing a cell sample with a promoter; and a culture step of culturing, inside a spheroid formation-culture vessel, the mixture obtained in the preparation step, in which the promoter is a polymer in which one or more selected from the group consisting of D-glucosamine, D-galactosamine, D-glucuronic acid, L-iduronic acid, and D-galactose are polymerized.
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公开(公告)号:US20230119663A1
公开(公告)日:2023-04-20
申请号:US17907187
申请日:2021-03-25
Applicant: OSAKA UNIVERSITY
Inventor: Michiya MATSUSAKI , Donghee KANG
Abstract: [Problem] To provide a method for producing an artificial three-dimensional muscle tissue, said method enabling stable, efficient and easy production of a muscle tissue, and an artificial three-dimensional muscle tissue produced by this method. [Solution] A method for producing an artificial three-dimensional muscle tissue, said method comprising steps of (i) forming a three-dimensional muscle tissue precursor, which is configured from a first muscle tissue support, a second muscle tissue support and muscle cells, by linearly arranging the muscle cells on the first muscle tissue support in such a manner that the muscle cells are located close to the first muscle tissue support at one end of the line and close to the second muscle tissue support at the other end of the line, and (ii) culturing the three-dimensional muscle tissue precursor to give an artificial three-dimensional muscle tissue, and an artificial three-dimensional muscle tissue obtained by this method.
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公开(公告)号:US20220341825A1
公开(公告)日:2022-10-27
申请号:US17761052
申请日:2020-09-24
Applicant: OSAKA UNIVERSITY
Inventor: Michiya MATSUSAKI
IPC: G01N1/30
Abstract: A method for transparentizing biological tissue in a living state, which comprises applying a solution to the biological tissue, the solution containing one or more compounds selected from the group consisting of a nucleotide, a nucleotide derivative, a macromolecule containing a nucleotide or a nucleotide derivative, and a nucleotide polymer; a composition for transparentizing biological tissue in a living state, which comprises the aforementioned compound; and a kit for transparentizing biological tissue in a living state, which comprises the aforementioned compound.
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公开(公告)号:US20210115377A1
公开(公告)日:2021-04-22
申请号:US17050734
申请日:2019-05-07
Applicant: TOPPAN PRINTING CO., LTD. , OSAKA UNIVERSITY
Inventor: Shiro KITANO , Shinji IRIE , Michiya MATSUSAKI
Abstract: The present invention relates to an extracellular-matrix-containing composition comprising: a fragmented extracellular matrix component; and an aqueous medium.
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公开(公告)号:US20190093070A1
公开(公告)日:2019-03-28
申请号:US15998571
申请日:2017-01-31
Applicant: OSAKA UNIVERSITY
Inventor: Michiya MATSUSAKI
Abstract: Provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (a) a step for forming a vascular system structure template using a gel; (b) a step for forming a three-dimensional tissue in the vicinity of the template; (c) a step for dissolving the template using a cationic solution; and (d) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells in a void remaining after the dissolution of the template. Also provided is a method for producing a three-dimensional tissue having a vascular system structure, said method comprising: (i) a step for forming a vascular system structure template using a gel; (ii) a step for seeding vascular endothelial cells and/or lymphatic vessel endothelial cells on the template; (iii) a step for forming a three-dimensional tissue in the vicinity of the cells seeded above; and (iv) a step for dissolving the template using a cationic solution. Also provided is a three-dimensional tissue comprising a gel which has a vascular system structure.
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