公开(公告)号：US20250057974A1

公开(公告)日：2025-02-20

申请号：US18723980

申请日：2022-12-14

Applicant: TOPPAN Holdings Inc. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Masahiko NAKAMOTO ,  Shiro KITANO

IPC: A61K47/69 ,  A61K9/06 ,  A61K47/62 ,  C08J3/24 ,  C08L89/00

Abstract: The present invention relates to a method for changing a hydrogel volume, the method including a step A of bringing an ionic polymer into contact with a hydrogel, which has a site having decomposition activity for the ionic polymer and a cross-linkable functional group that is capable of cross-linking with the ionic polymer through electrostatic interaction, to allow the cross-linkable functional group to cross-link with the ionic polymer, and reducing a volume of the hydrogel; and a step B of decomposing the ionic polymer cross-linking with the hydrogel by using the site having decomposition activity and discharging at least part of a decomposition product of the ionic polymer from the hydrogel to increase the hydrogel volume.

公开(公告)号：US20240352198A1

公开(公告)日：2024-10-24

申请号：US18686227

申请日：2022-08-22

Applicant: OSAKA UNIVERSITY ,  TOPPAN HOLDINGS INC.

Inventor： Michiya MATSUSAKI ,  Donghee KANG

IPC: C08H1/06 ,  C08L89/06 ,  D01F4/00

CPC classification number: C08H1/06 ,  C08L89/06 ,  D01F4/00

Abstract: Provided are a method for producing a protein material without using an organic solvent, the method involving ejecting a solution containing a protein material raw material into a water-based medium and drying the ejected protein material raw material, and a protein material produced by the method.

公开(公告)号：US20250002865A1

公开(公告)日：2025-01-02

申请号：US18704322

申请日：2022-10-27

Applicant: TOPPAN Holdings Inc. ,  OSAKA UNIVERSITY ,  KYOTO PREFECTURAL PUBLIC UNIVERSITY CORPORATION

Inventor： Shiro KITANO ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: C12N5/071

Abstract: There is provided a method for producing a tissue construct comprising vascular endothelial cells, the method comprising incubating cells comprising at least adipose-derived stem cells from human in the presence of a TGFβ type I receptor inhibitor.

公开(公告)号：US20220145242A1

公开(公告)日：2022-05-12

申请号：US17430648

申请日：2021-03-25

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/00 ,  C07K14/78

Abstract: The present invention relates to an extracellular matrix-containing composition containing a fragmented extracellular matrix component and a compound bound or adsorbed to the fragmented extracellular matrix component.

公开(公告)号：US20220133952A1

公开(公告)日：2022-05-05

申请号：US17502496

申请日：2021-10-15

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shinji IRIE ,  Shiro KITANO

IPC: A61L27/24 ,  A61L27/44 ,  A61L27/54 ,  A61L31/04 ,  C12N5/00 ,  A61L27/38

Abstract: A method may comprises bringing cells suspended in an aqueous medium into contact with a plurality of fragmented collagen pieces and, after the cells brought into contact with the plurality of fragmented collagen pieces and the plurality of fragmented collagen pieces are concentrated, culturing the cells brought into contact with the fragmented collagen pieces, with the plurality of fragmented collagen pieces, to form a three-dimensional tissue.

公开(公告)号：US20190351098A1

公开(公告)日：2019-11-21

申请号：US16480114

申请日：2018-01-31

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Shinji IRIE ,  Shiro KITANO

IPC: A61L27/24 ,  C12N5/00 ,  A61L31/04 ,  A61L27/44 ,  A61L27/54

Abstract: Disclosed is a three-dimensional tissue comprising cells and collagen including endogenous collagen, wherein at least a portion of the cells is adhered to the collagen, and the content of the collagen is from 10 wt % to 90 wt % based on the three-dimensional tissue.

公开(公告)号：US20220411750A1

公开(公告)日：2022-12-29

申请号：US17777786

申请日：2020-11-17

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Yasuyuki HIRAOKA ,  Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/071

Abstract: The present invention relates to a cell structure including cells containing at least hepatocytes and vascular endothelial cells, and extracellular matrix component, wherein the extracellular matrix components are disposed between the cells, and a liver sinusoidal network is provided between the cells.

公开(公告)号：US20180355322A1

公开(公告)日：2018-12-13

申请号：US16104533

申请日：2018-08-17

Applicant: TOPPAN PRINTING CO., LTD. ,  OSAKA UNIVERSITY

Inventor： Kei TSUKAMOTO ,  Shinji IRIE ,  Michiya MATSUSAKI

IPC: C12N5/09

CPC classification number: C12N5/0693 ,  C12M3/00 ,  C12N2501/91

Abstract: A method of promoting spheroid formation, including: a preparation step of preparing a mixture obtained by mixing a cell sample with a promoter; and a culture step of culturing, inside a spheroid formation-culture vessel, the mixture obtained in the preparation step, in which the promoter is a polymer in which one or more selected from the group consisting of D-glucosamine, D-galactosamine, D-glucuronic acid, L-iduronic acid, and D-galactose are polymerized.

公开(公告)号：US20230119663A1

公开(公告)日：2023-04-20

申请号：US17907187

申请日：2021-03-25

Applicant: OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI ,  Donghee KANG

IPC: C12N5/071 ,  B33Y10/00 ,  B33Y80/00 ,  A23P20/20 ,  A23L13/00

Abstract: [Problem] To provide a method for producing an artificial three-dimensional muscle tissue, said method enabling stable, efficient and easy production of a muscle tissue, and an artificial three-dimensional muscle tissue produced by this method. [Solution] A method for producing an artificial three-dimensional muscle tissue, said method comprising steps of (i) forming a three-dimensional muscle tissue precursor, which is configured from a first muscle tissue support, a second muscle tissue support and muscle cells, by linearly arranging the muscle cells on the first muscle tissue support in such a manner that the muscle cells are located close to the first muscle tissue support at one end of the line and close to the second muscle tissue support at the other end of the line, and (ii) culturing the three-dimensional muscle tissue precursor to give an artificial three-dimensional muscle tissue, and an artificial three-dimensional muscle tissue obtained by this method.

公开(公告)号：US20220341825A1

公开(公告)日：2022-10-27

申请号：US17761052

申请日：2020-09-24

Applicant: OSAKA UNIVERSITY

Inventor： Michiya MATSUSAKI

IPC: G01N1/30

Abstract: A method for transparentizing biological tissue in a living state, which comprises applying a solution to the biological tissue, the solution containing one or more compounds selected from the group consisting of a nucleotide, a nucleotide derivative, a macromolecule containing a nucleotide or a nucleotide derivative, and a nucleotide polymer; a composition for transparentizing biological tissue in a living state, which comprises the aforementioned compound; and a kit for transparentizing biological tissue in a living state, which comprises the aforementioned compound.