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    OLIGONUCLEOTIDES CAPABLE OF DISCRIMINATING BETWEEN NUCLEIC ACID SEQUENCES THAT COMPRISE A CONSERVED SEQUENCE
    11.
    发明申请
    OLIGONUCLEOTIDES CAPABLE OF DISCRIMINATING BETWEEN NUCLEIC ACID SEQUENCES THAT COMPRISE A CONSERVED SEQUENCE 审中-公开
    在包含保守序列的核酸序列之间可分辨的寡核苷酸

    公开(公告)号：US20110319283A1

    公开(公告)日：2011-12-29

    申请号：US13130946

    申请日：2009-11-24

    申请人： Andrew Thompson

    发明人： Andrew Thompson

    IPC分类号： C40B30/04 ,  C12Q1/68 ,  C07H21/00 ,  C40B40/06 ,  B82Y15/00

    CPC分类号： C12Q1/6816 ,  C12Q2565/501 ,  C12Q2525/161 ,  C12Q2525/119

    摘要： The invention provides inter alia an oligonucleotide (or “probe”) able to discriminate between a nucleic acid target and a nucleic acid variant thereof (for example, mRNA splice variants, or chimeric gene and corresponding parent gene mRNAs) in which the target and variant that share at least one domain of conserved or identical sequence. The oligonucleotide in one aspect has a first portion and a second portion flanking a portion junction, wherein the first portion comprises at least a first discontinuity relative to the first domain of a target sequence and the second portion comprises at least a second discontinuity relative to a second domain of a target sequence, each discontinuity comprising or consisting of a sequence mismatch and/or a non-nucleotide spacer.

    摘要翻译： 本发明特别提供了能够区分核酸靶和其核酸变体（例如，mRNA剪接变体，或嵌合基因和相应的亲本基因mRNA）的寡核苷酸（或“探针”），其中靶和变体 其共享至少一个保守或相同序列的结构域。 一方面的寡核苷酸具有位于部分结的侧翼的第一部分和第二部分，其中第一部分包括相对于靶序列的第一结构域的至少第一不连续部分，并且第二部分包括相对于靶序列的至少第二不连续性 靶序列的第二结构域，每个不连续性包含序列错配和/或非核苷酸间隔物或由序列错配组成。

    Systems and methods for extracting lipids from and dehydrating wet algal biomass
    13.
    发明授权
    Systems and methods for extracting lipids from and dehydrating wet algal biomass 有权
    从湿藻类生物质提取脂质和脱水的系统和方法

    公开(公告)号：US07868195B2

    公开(公告)日：2011-01-11

    申请号：US12610134

    申请日：2009-10-30

    申请人： Daniel Fleischer ,  Marko Jukic ,  Andrew Thompson ,  Guido Radaelli

    发明人： Daniel Fleischer ,  Marko Jukic ,  Andrew Thompson ,  Guido Radaelli

    IPC分类号： C11B1/00

    CPC分类号： C11B1/10 ,  C11B1/106

    摘要： Exemplary methods include centrifuging a wet algal biomass to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a centrifuged algal biomass, mixing the centrifuged algal biomass with an amphiphilic solvent to result in a mixture, heating the mixture to result in a dehydrated, defatted algal biomass, separating the amphiphilic solvent from the dehydrated, defatted algal biomass to result in amphiphilic solvent, water and lipids, evaporating the amphiphilic solvent from the water and the lipids, and separating the water from the lipids. The amphiphilic solvent may be selected from a group consisting of acetone, methanol, ethanol, isopropanol, butanone, dimethyl ether, and propionaldehyde. Other exemplary methods include filtering a wet algal biomass through a membrane to increase a solid content of the wet algal biomass to between approximately 10% and 40% to result in a filtered algal biomass.

    摘要翻译： 示例性方法包括将湿藻类生物质离心以将湿藻类生物质的固体含量提高到约10％至40％之间，以产生离心的藻类生物质，将离心的藻类生物质与两亲溶剂混合以产生混合物，加热 混合物导致脱水的脱脂藻类生物质，将两亲溶剂与脱水的脱脂藻类生物质分离以产生两亲溶剂，水和脂质，从水和脂质中蒸发出两亲溶剂，并将水从 脂质 两亲溶剂可以选自丙酮，甲醇，乙醇，异丙醇，丁酮，二甲醚和丙醛。 其他示例性方法包括通过膜过滤湿藻类生物质以将湿藻类生物质的固体含量增加至约10％至40％之间，以产生过滤的藻类生物质。

    PEPTIDE / PROTEIN IDENTIFICATION USING PHOTOREACTIVE CARRIERS FOR THE IMMOBILISATION OF THE LIGANDS
    14.
    发明申请
    PEPTIDE / PROTEIN IDENTIFICATION USING PHOTOREACTIVE CARRIERS FOR THE IMMOBILISATION OF THE LIGANDS 审中-公开
    肽类/蛋白质鉴定使用光电子载体进行配体化

    公开(公告)号：US20100173793A1

    公开(公告)日：2010-07-08

    申请号：US12063803

    申请日：2006-08-17

    申请人： Suzanne Jane Dilly ,  Paul Christopher Taylor ,  Andrew James Clark ,  Andrew Marsh ,  Richard Napier ,  Andrew Thompson

    发明人： Suzanne Jane Dilly ,  Paul Christopher Taylor ,  Andrew James Clark ,  Andrew Marsh ,  Richard Napier ,  Andrew Thompson

    IPC分类号： C40B30/04 ,  C07D229/02 ,  C08G63/91 ,  C08G69/48 ,  B01L3/00 ,  C40B40/10

    CPC分类号： G01N33/6845 ,  G01N33/54353 ,  G01N2500/04

    摘要： The invention provides a method of identifying a peptide or protein capable of binding a ligand which comprises: (i) providing a support, the support comprising a photoreactive group; (ii) reacting the photoreactive group with a ligand to attach the ligand to the support and produce a supported ligand; (iii) providing an expression library comprising a plurality of members, each member expressing a different peptide or protein; (iv) screening the expression library to identify one or more peptides or proteins which bind to the ligand; (v) isolating the member or each member of the library which expresses a peptide or protein which binds to the ligand; and (vi) identifying the peptide or protein which binds to the ligand. Supported photo-reactive compounds are disclosed comprising a photo-reactive group attached to a support via a spacer and a dendritic group, the dendritic group comprising attached thereto, optionally via a spacer, at least one further photo-reactive group and/or a second functional group. Compounds comprising photo-reactive groups attached to a support and a protein resistant group attached to the support are also provided, together with kits for carrying out the method of the invention.

    摘要翻译： 本发明提供了鉴定能够结合配体的肽或蛋白质的方法，其包括：（i）提供载体，所述载体包含光反应性基团; （ii）使光反应性基团与配体反应以将配体连接到载体上并产生负载的配体; （iii）提供包含多个成员的表达文库，每个成员表达不同的肽或蛋白质; （iv）筛选表达文库以鉴定结合配体的一种或多种肽或蛋白质; （v）分离表达与配体结合的肽或蛋白质的文库的成员或每个成员; 和（vi）鉴定与配体结合的肽或蛋白质。 公开了支持的光反应性化合物，其包括通过间隔基和树枝状基团连接到载体上的光反应性基团，所述树枝状基团任选地经由间隔基，至少一个另外的光反应性基团和/或第二 功能组。 还提供了包含连接到载体上的光反应性基团和与载体连接的抗蛋白质基团的化合物以及用于实施本发明方法的试剂盒。

    Method for characterizing polypeptides
    17.
    发明授权
    Method for characterizing polypeptides 失效
    表征多肽的方法

    公开(公告)号：US07163803B2

    公开(公告)日：2007-01-16

    申请号：US10479843

    申请日：2002-06-07

    申请人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

    发明人： Christian Hamon ,  Andrew Thompson ,  Thomas Neumann ,  Robert Johnstone ,  Abdul Karim Abed Mohammed

    IPC分类号： C12Q1/37

    CPC分类号： G01N33/6848 ,  C07K1/12 ,  G01N33/6821

    摘要： Provided is a method for characterizing a polypeptide, which method comprises the steps of: (a) optionally reducing cysteine disulphide bridges in the polypeptide to form free thiols, and capping the free thiols; (b) cleaving the polypeptide with a sequence specific cleavage reagent to form peptide fragments; (c) optionally deactivating the cleavage reagent; (d) capping one or more ε-amino groups that are present with a lysine reactive agent; (e) analyzing peptide fragments by mass spectrometry to form a mass fingerprint for the polypeptide; and (f) determining the identity of the polypeptide from the mass fingerprint.

    摘要翻译： 提供了表征多肽的方法，该方法包括以下步骤：（a）任选地减少多肽中的半胱氨酸二硫键形成游离硫醇，并封端游离硫醇; （b）用序列特异性切割试剂切割多肽以形成肽片段; （c）任选地使切割试剂失活; （d）封端与赖氨酸反应剂一起存在的一个或多个ε-氨基; （e）通过质谱分析肽片段以形成多肽的质量指纹图谱; 和（f）从质量指纹图谱确定多肽的身份。

    ALS CAMERA SYSTEM
    20.
    发明申请
    ALS CAMERA SYSTEM 有权

    公开(公告)号：US20220294947A1

    公开(公告)日：2022-09-15

    申请号：US17691534

    申请日：2022-03-10

    申请人： Kevin Thomas ,  Andrew Thompson

    发明人： Kevin Thomas ,  Andrew Thompson

    IPC分类号： H04N5/225 ,  G06T7/00 ,  G02B7/00

    摘要： A system for detecting foreign materials comprising an alternative light source (ALS), one or more filters operationally associated with the ALS, one or more camera lenses operationally associated with the ALS and the filters, one or more processors, a computer readable memory, and a computer readable storage medium operatively associated with a computing device operatively associated with the ALS, one or more sensors operationally associated with the computing device and the ALS, wherein the sensors detect the presence of the filter and a camera operationally associated with the lens and the computing device.