利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

30 条记录 (耗时 0.028 秒)

    Production of interferon
    12.
    发明授权
    Production of interferon 失效
    生产干扰素

    公开(公告)号：US5541312A

    公开(公告)日：1996-07-30

    申请号：US425934

    申请日：1982-09-28

    申请人： Michel Revel ,  Pierre Tiollais

    发明人： Michel Revel ,  Pierre Tiollais

    IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C07K14/54 ,  C07K14/565 ,  C12N15/00 ,  C12N15/22 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91

    CPC分类号： C07K14/5412 ,  C07K14/565

    摘要： The present invention .relates to a process to isolate genetic material (DNA) containing the nucleotide sequence coding for interferon in human fibroblastic cells which comprises cultivating cells producing interferon when exposed to an inducer of interferon, exposing same to such inducer, extracting messenger RNA from said induced cells, purifying the interferon messenger RNA, transcribing the messenger RNA into DNA and cloning the DNA in a suitable vector. Preferred cells are human diploid foreskin cells. The invention further relates to a process for engineering a bacterial strain to produce interferon polypeptide which comprises introducing a cloned interferon DNA into a suitable vector-carrier. A preferred vector-carrier is E. coli. The invention also relates to the mRNA of human interferon in highly purified form, to the mRNA of human interferon in .beta.1 highly purified form, to the mRNA of human interferon in .sym.2 highly purified form, to the DNA coding for a polypeptide having interferon activity, insertable in a vector, such as plasmid pBR322, and also to human interferon .beta.1 in highly purified form, and human interferon .beta.2 in highly purified form.

    摘要翻译： 本发明涉及分离含有人成纤维细胞中编码干扰素的核苷酸序列的遗传物质（DNA）的方法，其包括在暴露于干扰素诱导剂时培养产生干扰素的细胞，将其暴露于这种诱导物，从其中提取信使RNA 所述诱导细胞，纯化干扰素信使RNA，将信使RNA转录成DNA并将DNA克隆在合适的载体中。 优选的细胞是人二倍体包皮细胞。 本发明还涉及一种用于工程化细菌菌株以产生干扰素多肽的方法，其包括将克隆的干扰素DNA引入合适的载体载体中。 优选的载体载体是大肠杆菌。 本发明还涉及高度纯化形式的人干扰素与β1高度纯化形式的人干扰素的mRNA与（+）2高度纯化形式的人干扰素mRNA相关的编码多肽的DNA 具有干扰素活性，可插入载体，例如质粒pBR322，以及高度纯化形式的人干扰素β1，以及高度纯化形式的人干扰素β2。

    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor
    13.
    发明授权
    Steroid/thyroid hormone receptor-related gene, which is inappropriately expressed in human heptocellular carcinoma, and which is a retinoic acid receptor 失效
    类固醇/甲状腺激素受体相关基因，其在人肝细胞癌中不适当地表达，并且其是视黄酸受体

    公开(公告)号：US5468617A

    公开(公告)日：1995-11-21

    申请号：US190555

    申请日：1994-02-02

    申请人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    发明人： Hughes Blaudin De The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne DeJean ,  Nigel Brand ,  Martin Petkovich ,  Andree Krust ,  Pierre Chambon

    IPC分类号： C07K14/705 ,  C12Q1/68 ,  G01N33/48

    CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158 ,  Y10S530/828

    摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-.beta.. The RAR-.beta. gene is transcriptionally up-regulated by retinoic acid (RA) and its promoter region may contain a RARE (retinoic acid responsive element).

    摘要翻译： 将以前分离的乙肝病毒（HBV）整合到与肝细胞癌（HCC）连接的147bp的细胞DNA片段中作为探针，从人肝cDNA文库克隆相应的互补DNA。 核苷酸序列分析显示，已命名为hap的细胞基因的整体结构与DNA结合激素受体的结构相似。 7例肝癌和肝癌细胞系中有6例表达2.5 kb的hap mRNA，在正常成人和胎儿肝脏中检测不到，但存在于所有非肝脏组织中。 低严格杂交实验揭示了人类基因组中hap相关基因的存在。 克隆的DNA序列可用于制备纯hap蛋白，并用作检测和分离互补DNA和RNA序列的探针。 hap蛋白是鉴定为RAR-β的视黄酸（RA）受体。 RAR-β基因由视黄酸（RA）转录上调，其启动子区域可能含有RARE（视黄酸反应元件）。

    Identification of a synthetic ligand for retinoic acid receptor beta
    14.
    发明授权
    Identification of a synthetic ligand for retinoic acid receptor beta 失效
    鉴定视黄酸受体β的合成配体

    公开(公告)号：US07622266B2

    公开(公告)日：2009-11-24

    申请号：US11384481

    申请日：2006-03-21

    申请人： Hugues Blaudin de The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean

    发明人： Hugues Blaudin de The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean

    IPC分类号： G01N33/566 ,  C07K14/705

    CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158

    摘要： A method for identifying a synthetic ligand for retinoic acid receptor β comprises providing a sample, including a synthetic compound, exposing the sample to cultured cells, wherein the cultured cells comprise RARβ, determining if transcriptional expression of a gene encoding RARβ is upregulated compared to transcriptional expression of a control gene, and choosing a sample that upregulates the expression of RARβ as being a synthetic ligand for RARβ.

    摘要翻译： 用于鉴定视黄酸受体β的合成配体的方法包括提供包括合成化合物的样品，将样品暴露于培养细胞，其中培养的细胞包含RARbeta，确定编码RARbeta的基因的转录表达是否与转录相比上调 控制基因的表达，并选择上调RARbeta作为RARbeta的合成配体的表达的样品。

    Steroid/thyroid hormone receptor-related gene which is inappropriately expressed in human hepatocellular carcinoma and which is a retinoic acid receptor
    16.
    发明授权
    Steroid/thyroid hormone receptor-related gene which is inappropriately expressed in human hepatocellular carcinoma and which is a retinoic acid receptor 失效
    在人肝细胞癌中不适当表达的类固醇/甲状腺激素受体相关基因，其是视黄酸受体

    公开(公告)号：US06531585B1

    公开(公告)日：2003-03-11

    申请号：US07649342

    申请日：1991-02-01

    申请人： Hugues Blaudin de The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean

    发明人： Hugues Blaudin de The ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean

    IPC分类号： C12N1512

    CPC分类号： C07K14/70567 ,  C12Q1/6876 ,  C12Q2600/158

    摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all nonhepactic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences. The hap protein is a retinoic acid (RA) receptor identified as RAR-&bgr;.

    摘要翻译： 将以前分离的乙肝病毒（HBV）整合到与肝细胞癌（HCC）连接的147bp的细胞DNA片段中作为探针，从人肝cDNA文库克隆相应的互补DNA。 核苷酸序列分析显示，已命名为hap的细胞基因的整体结构与DNA结合激素受体的结构相似。 七个肝癌和肝癌细胞系中有六个表达了2.5kb的hap mRNA，在正常成年和胎儿肝脏中是不可检测的，但存在于所有非肝脏组织中。 低严格杂交实验揭示了人类基因组中hap相关基因的存在。 克隆的DNA序列可用于制备纯hap蛋白，并用作检测和分离互补DNA和RNA序列的探针。 hap蛋白是鉴定为RAR-β的视黄酸（RA）受体。

    Production of interferon
    17.
    发明授权
    Production of interferon 失效

    公开(公告)号：US5468607A

    公开(公告)日：1995-11-21

    申请号：US208925

    申请日：1980-11-20

    申请人： Michel Revel ,  Pierre Tiollais

    发明人： Michel Revel ,  Pierre Tiollais

    IPC分类号： C12N15/09 ,  C07H21/02 ,  C07H21/04 ,  C07K14/54 ,  C07K14/565 ,  C12N15/00 ,  C12N15/22 ,  C12P19/34 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12R1/91 ,  C12N15/70 ,  C12Q1/68

    CPC分类号： C07K14/5412 ,  C07K14/565

    摘要： The present invention relates to a process to isolate genetic material (DNA) containing the nucleotide sequence coding for interferon in human fibroblastic cells which comprises cultivating cells producing interferon when exposed to an inducer of interferon, exposing same to such inducer, extracting messenger RNA from said induced cells, purifying the interferon messenger RNA, transcribing the messenger RNA into DNA and cloning the DNA in a suitable vector. Preferred cells are human diploid foreskin cells. The invention further relates to a process for engineering a bacterial strain to produce interferon polypeptide which comprises introducing a cloned interferon DNA into a suitable vector-carrier. A preferred vector-carrier is E. coli. The invention also relates to the mRNA of human interferon in highly purified form, to the mRNA of human interferon in .beta.1 highly purified form, to the mRNA of human interferon in .beta.2 highly purified form, to the DNA coding for a polypeptide having interferon activity, insertable in a vector, such as plasmid pBR322, and also to human interferon .beta.1 in highly purified form, and human interferon .beta.2 in highly purified form.

    Steroid/thyroid hormone receptor-related gene inappropriately expressed in human hepatocellular carcinoma
    18.
    发明授权
    Steroid/thyroid hormone receptor-related gene inappropriately expressed in human hepatocellular carcinoma 失效
    类固醇/甲状腺激素受体相关基因在人肝细胞癌中不适当表达

    公开(公告)号：US5149781A

    公开(公告)日：1992-09-22

    申请号：US209009

    申请日：1988-06-20

    申请人： Hugues Blaudin de THE ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean

    发明人： Hugues Blaudin de THE ,  Agnes Marchio ,  Pierre Tiollais ,  Anne Dejean

    IPC分类号： C12N15/09 ,  A61K38/00 ,  A61P35/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C07K14/705 ,  C12N1/21 ,  C12P21/02 ,  C12Q1/68

    CPC分类号： C12Q1/6876 ,  C07K14/70567 ,  C12Q2600/158

    摘要： A previously isolated hepatitis B virus (HBV) integration in a 147 bp cellular DNA fragment linked to hepatocellular carcinoma (HCC) was used as a probe to clone the corresponding complementary DNA from a human liver cDNA library. Nucleotide sequence analysis revealed that the overall structure of the cellular gene, which has been named hap, is similar to that of the DNA-binding hormone receptors. Six out of seven hepatoma and hepatoma-derived cell-lines express a 2.5 kb hap mRNA species which is undetectable in normal adult and fetal livers, but present in all non-hepatic tissues analyzed. Low stringency hybridization experiments revealed the existence of hap related genes in the human genome. The cloned DNA sequence is useful in the preparation of pure hap protein and as a probe in the detection and isolation of complementary DNA and RNA sequences.

    摘要翻译： 将以前分离的乙肝病毒（HBV）整合到与肝细胞癌（HCC）连接的147bp的细胞DNA片段中作为探针，从人肝cDNA文库克隆相应的互补DNA。 核苷酸序列分析显示，已命名为hap的细胞基因的整体结构与DNA结合激素受体的结构相似。 7例肝癌和肝癌细胞系中有6例表达2.5 kb的hap mRNA，在正常成人和胎儿肝脏中检测不到，但存在于所有非肝脏组织中。 低严格杂交实验揭示了人类基因组中hap相关基因的存在。 克隆的DNA序列可用于制备纯hap蛋白，并用作检测和分离互补DNA和RNA序列的探针。

    Vectors for the insertion therein of foreign DNA fragments, according to any translation phase
    19.
    发明授权
    Vectors for the insertion therein of foreign DNA fragments, according to any translation phase 失效
    根据任何翻译阶段在其中插入外来DNA片段的载体

    公开(公告)号：US4371625A

    公开(公告)日：1983-02-01

    申请号：US93270

    申请日：1979-11-13

    申请人： Pierre Tiollais

    发明人： Pierre Tiollais

    IPC分类号： C12N15/09 ,  C12N15/00 ,  C12P19/34 ,  C12N1/00

    CPC分类号： C12N15/00

    摘要： The invention pertains to a set of vectors (or of DNA fragments to be inserted in a vector) which distinguish from one another in that, taking into account one vector in which the number of pairs of bases between the reading initiation point of the vector and a point corresponding to the first pair of bases of a recognition site corresponding to a predetermined restriction enzyme, the two other vectors comprise between the corresponding points additional groups of pairs of bases comprising respectively two pairs of bases on the one hand and either one or four pairs of bases on the other hand, plus possibly whole numbers of triplets. On inserting a determined DNA fragment of which the expression is sought in bacteria in the three vectors, the reading of said DNA fragment will occur in phase as concerns one of the so modified vectors after transfection of bacteria therewith.

    摘要翻译： 本发明涉及一组彼此区分的载体（或插入到载体中的DNA片段），其中考虑到一个载体，其中载体的读取起始点与碱基对的数量对 对应于对应于预定限制酶的识别位点的第一对碱基的点，另外两个载体在相应的点之间包括一对另外的一对碱基，其一对分别包括两对碱基和一个或四个碱基 另一方面是一对基地，还有可能是整数的三胞胎。 在三个载体中在细菌中插入其中寻求表达的确定的DNA片段时，所述DNA片段的读取将在转染细菌后与所修饰的载体之一相同地进行。