公开(公告)号：US10717990B2

公开(公告)日：2020-07-21

申请号：US14319171

申请日：2014-06-30

Applicant: President and Fellows of Harvard College

Inventor： Prashant G. Mali ,  George M. Church ,  Luhan Yang

IPC: C12Q1/68 ,  C12N9/14 ,  C12N9/22 ,  C12N9/52 ,  C12N15/00 ,  C07H21/02 ,  C07H21/04 ,  A61K38/43 ,  A61K38/46 ,  A61K38/47 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US09260723B2

公开(公告)日：2016-02-16

申请号：US14319255

申请日：2014-06-30

Applicant: President and Fellows of Harvard College

Inventor： Prashant G. Mali ,  George M. Church ,  Luhan Yang

IPC: C12N15/85 ,  C12N9/14 ,  C12N9/22 ,  C07H21/02 ,  C07H21/04 ,  C12N15/87 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20160002670A1

公开(公告)日：2016-01-07

申请号：US14790147

申请日：2015-07-02

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/90

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20240294939A1

公开(公告)日：2024-09-05

申请号：US18663233

申请日：2024-05-14

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/63 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N15/90

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20240279677A1

公开(公告)日：2024-08-22

申请号：US18619354

申请日：2024-03-28

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/63 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N15/90

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11535863B2

公开(公告)日：2022-12-27

申请号：US16439840

申请日：2019-06-13

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11359211B2

公开(公告)日：2022-06-14

申请号：US16397423

申请日：2019-04-29

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11236359B2

公开(公告)日：2022-02-01

申请号：US15042573

申请日：2016-02-12

Applicant: President and Fellows of Harvard College

Inventor： Prashant G. Mali ,  George M. Church ,  Luhan Yang

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20200024618A1

公开(公告)日：2020-01-23

申请号：US16441209

申请日：2019-06-14

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Kevin M. Esvelt

IPC: C12N15/90 ,  C12N15/113 ,  C12N15/10 ,  C12N15/63 ,  C12N9/22 ,  C12N15/11

Abstract: Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

公开(公告)号：US20150232833A1

公开(公告)日：2015-08-20

申请号：US14701912

申请日：2015-05-01

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Prashant G. Mali ,  George M. Church ,  Luhan Yang

IPC: C12N15/10

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.