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39 条记录 (耗时 0.031 秒)

    Near infrared chemiluminescent acridinium compounds and uses thereof
    11.
    发明授权
    Near infrared chemiluminescent acridinium compounds and uses thereof 有权
    近红外化学发光吖啶化合物及其用途

    公开(公告)号：US07083986B2

    公开(公告)日：2006-08-01

    申请号：US10006421

    申请日：2001-12-06

    申请人： Anand Natrajan ,  Qingping Jiang ,  David Sharpe ,  Say-Jong Law

    发明人： Anand Natrajan ,  Qingping Jiang ,  David Sharpe ,  Say-Jong Law

    IPC分类号： G01N33/533 ,  G01N33/78 ,  G01N33/554 ,  G01N33/569 ,  C12Q1/68

    CPC分类号： G01N33/582 ,  C07D219/04 ,  Y10S436/80 ,  Y10S436/815 ,  Y10S436/817 ,  Y10S530/812 ,  Y10T436/13

    摘要： A method for the detection or quantitation of an analyte in a biological sample is disclosed. The method comprises the steps of selecting a biological sample suspected of containing a target analyte and binding the target analyte in the sample to a binding partner conjugated to an acridinium compound, wherein the acridinium compound comprises an acridinium nucleus having an electron-donating substituent directly attached to the acridinium nucleus at the C2 position. Chemiluminescent acridinium compounds useful in the method have emission maxima close to or in the near infrared (NIR) region (>590 nm). These chemiluminescent acridinium compounds when used in conjunction with short wavelength-emitting acridinium esters (with emission maxima below 450 nm) can be highly useful labels for the simultaneous detection of multiple target analytes in a single assay.

    摘要翻译： 公开了用于检测或定量生物样品中的分析物的方法。 该方法包括以下步骤：选择怀疑含有目标分析物并将样品中的目标分析物结合到与吖啶鎓化合物结合的结合对象的生物样品，其中所述吖啶鎓化合物包含具有直接连接的给电子取代基的吖啶核 到C> 2位上的吖啶核。 可用于该方法的化学发光吖啶鎓化合物具有接近或在近红外（NIR）区域（> 590nm）中的发射最大值。 当与短波长发射的吖啶酯（发射最大值低于450nm）结合使用时，这些化学发光吖啶鎓化合物可以是在单次测定中同时检测多种靶分析物的非常有用的标记。

    Chemiluminescent energy transfer conjugates and their uses as labels in binding assays
    12.
    发明授权
    Chemiluminescent energy transfer conjugates and their uses as labels in binding assays 失效
    化学发光能量转移共轭物及其用作结合测定中的标记物

    公开(公告)号：US6165800A

    公开(公告)日：2000-12-26

    申请号：US86003

    申请日：1998-05-27

    申请人： Qingping Jiang ,  Jun Xi ,  Anand Natrajan ,  David Sharpe ,  Marcus Baumann ,  Rolf Hilfiker ,  Erika Schmidt ,  Paul Senn ,  Fritz Thommen ,  Adrian Waldner ,  Alex Alder ,  Say-Jong Law

    发明人： Qingping Jiang ,  Jun Xi ,  Anand Natrajan ,  David Sharpe ,  Marcus Baumann ,  Rolf Hilfiker ,  Erika Schmidt ,  Paul Senn ,  Fritz Thommen ,  Adrian Waldner ,  Alex Alder ,  Say-Jong Law

    IPC分类号： C09B11/04 ,  C09B15/00 ,  C09K11/07 ,  G01N33/533 ,  G01N33/542 ,  G01N33/58 ,  C07D227/00 ,  G01N33/553

    CPC分类号： C09B15/00 ,  C09B11/04 ,  G01N33/533 ,  G01N33/542 ,  G01N33/582 ,  Y10S436/80 ,  Y10S436/816 ,  Y10S436/817

    摘要： A new class of chemiluminescent acridinium or benzacridinium compounds is disclosed by virtue of forming an intramolecular energy transfer conjugate (ETC) between the acridinium or benzacridinium compound and a luminophore. A method of extending the emission wavelengths of acridinium or benzacridinium esters in order to further reduce or eliminate the emission spectral overlap between the parent polysubstituted aryl Acridinium Esters (DMAE) and Benzacridinium Esters (LEAE) is disclosed. The ETC's retain the unique desired properties of acridinium or benzacridinium compounds including complete light emission in very short period of time, monophasic emission spectrum, simplicity of triggering mechanism, ability of labeling the biological molecules of interest to form a tracer, and good stability. Additionally, the range of the emission spectrum of an acridinium or benzacridinium compound can now be shifted at will and at longer leap through the choice of a luminophore as the integral part of an ETC molecule. Disclosed are chemiluminescent labeled conjugates comprising an acridinium or benzacridinium moiety covalently attached to a luminophore via a spacer, said moiety further conjugated to a biological molecule of interest, wherein said spacer is of an appropriate length to allow the excited species generated from said moiety to transfer energy efficiently to said luminophore, resulting in the emission of light in the spectral region of said luminophore. Also disclosed are binding assays using said conjugates, test kits comprising said conjugates and methods of preparing the conjugates.

    摘要翻译： 通过在吖啶鎓或苯并吖啶鎓化合物和发光体之间形成分子内能量转移缀合物（ETC），公开了一类新的化学发光吖啶鎓或苯并吖啶鎓化合物。 公开了延长吖啶鎓或苯并吖啶酯的发射波长以进一步减少或消除母体多取代芳基吖啶酯（DMAE）和苯并吖啶酯（LEAE）之间的发射光谱重叠的方法。 ETC保留吖啶鎓或苯并吖啶鎓化合物独特的所需性质，包括在很短的时间内完全发光，单相发射光谱，触发机制简单，标记生物分子的能力，形成示踪剂，以及良好的稳定性。 此外，吖啶鎓或苯并吖啶鎓化合物的发射光谱的范围现在可以随意移动，并且通过选择发光体作为ETC分子的组成部分而更长的跨越。 公开了化学发光标记的缀合物，其包含通过间隔基共价连接到发光体的吖啶鎓或苯并吖啶鎓部分，所述部分还与感兴趣的生物分子缀合，其中所述间隔物具有适当长度以允许从所述部分产生的激发物质转移 能量有效地发送到所述发光体，导致发光在所述发光体的光谱区域。 还公开了使用所述缀合物的结合测定法，包含所述缀合物的测试试剂盒和制备缀合物的方法。

    Method for enhancing signal release from liposomes using water-miscible alcohols
    19.
    发明授权
    Method for enhancing signal release from liposomes using water-miscible alcohols 失效
    使用水混溶性醇增强脂质体信号释放的方法

    公开(公告)号：US5093270A

    公开(公告)日：1992-03-03

    申请号：US356083

    申请日：1989-05-24

    申请人： Steve C. S. Chang ,  Say-Jong Law

    发明人： Steve C. S. Chang ,  Say-Jong Law

    IPC分类号： G01N33/543

    CPC分类号： G01N33/5436 ,  Y10S435/968 ,  Y10S436/829

    摘要： A method for releasing encapsulated marker molecules from within liposomes comprises contracting the liposomes with an effective amount of a water-miscible alcohol. The chemiluminescence of acridinium esters is enhanced by oxidizing the acridinium ester in the presence of an effective amount of a water-miscible alcohol.

    摘要翻译： 从脂质体内释放包封的标记分子的方法包括用有效量的水混溶性醇包封脂质体。 通过在有效量的水混溶性醇的存在下氧化吖啶酯来增强吖啶酯的化学发光。