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    Preparation of Nucleic Acid Templates for Solid Phase Amplification
    11.
    发明申请
    Preparation of Nucleic Acid Templates for Solid Phase Amplification 有权
    固相扩增核酸模板的制备

    公开(公告)号：US20100041561A1

    公开(公告)日：2010-02-18

    申请号：US12085508

    申请日：2006-11-24

    申请人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerado Turcatti ,  Colin Lloyd Barnes

    发明人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerado Turcatti ,  Colin Lloyd Barnes

    IPC分类号： C40B20/00 ,  C40B50/06

    CPC分类号： C12Q1/6855 ,  C12Q2565/537

    摘要： The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

    摘要翻译： 本发明涉及制备用于固相核酸扩增的模板构建体的方法以及在固相核酸扩增方法中使用该模板的方法。 该方法包括进行两个连接反应：（a）连接反应，其中一个或多个靶多核苷酸分子的第一末端连接到表面结合的衔接子多核苷酸分子，和（b）连接反应，其中溶液相适配器 将多核苷酸分子连接到所述靶多核苷酸分子的第二末端，以便产生连接到固体支持物上的一个或多个模板构建体。

    Preparation of nucleic acid templates for solid phase amplification
    12.
    发明授权
    Preparation of nucleic acid templates for solid phase amplification 有权
    制备用于固相扩增的核酸模板

    公开(公告)号：US08168388B2

    公开(公告)日：2012-05-01

    申请号：US12085508

    申请日：2006-11-24

    申请人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerardo Turcatti ,  Colin Lloyd Barnes

    发明人： Niall Anthony Gormley ,  Jonathan Mark Boutell ,  Gerardo Turcatti ,  Colin Lloyd Barnes

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6855 ,  C12Q2565/537

    摘要： The invention relates to a method of preparing template constructs for solid-phase nucleic acid amplification and to use of the templates in methods of solid-phase nucleic acid amplification. The method involves carrying out two ligation reactions: (a) a ligation reaction in which the first end of one or more target polynucleotide molecules are ligated to surface-bound adaptor polynucleotide molecules, and (b) a ligation reaction in which solution-phase adaptor polynucleotide molecules are ligated to the second end of said target polynucleotide molecules, in order to produce one or more template constructs attached to a solid support.

    摘要翻译： 本发明涉及制备用于固相核酸扩增的模板构建体的方法以及在固相核酸扩增方法中使用该模板的方法。 该方法包括进行两个连接反应：（a）连接反应，其中一个或多个靶多核苷酸分子的第一末端连接到表面结合的衔接子多核苷酸分子，和（b）连接反应，其中溶液相适配器 将多核苷酸分子连接到所述靶多核苷酸分子的第二末端，以便产生连接到固体支持物上的一个或多个模板构建体。

    End Modification to Prevent Over-Representation of Fragments
    14.
    发明申请
    End Modification to Prevent Over-Representation of Fragments 有权
    最终修改以防止片段过度表示

    公开(公告)号：US20090176662A1

    公开(公告)日：2009-07-09

    申请号：US12223761

    申请日：2007-02-07

    申请人： Roberto Rigatti ,  Niall Anthony Gormley ,  Helen Rachel Bignell

    发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Helen Rachel Bignell

    IPC分类号： C40B40/08 ,  C40B50/06

    CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12Q1/6855 ,  C12Q2525/119 ,  C12Q2525/186

    摘要： The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from whence the 5′ and 3′ modified library is generated is greatly reduced or prevented.

    摘要翻译： 本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，以及在固相核酸扩增方法中使用5'和3'修饰的模板文库。 特别地，本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，该文库在其5'末端和3'末端具有共同序列，其中主要“末端”序列的过表达 产生5'和3'修饰的文库的多核苷酸分子大大降低或防止。

    End modification to prevent over-representation of fragments
    16.
    发明授权
    End modification to prevent over-representation of fragments 有权
    结束修改以防止片段的过度表示

    公开(公告)号：US09012184B2

    公开(公告)日：2015-04-21

    申请号：US12223761

    申请日：2007-02-07

    申请人： Roberto Rigatti ,  Niall Anthony Gormley ,  Helen Rachel Bignell

    发明人： Roberto Rigatti ,  Niall Anthony Gormley ,  Helen Rachel Bignell

    IPC分类号： C12Q1/68 ,  C12N15/10

    CPC分类号： C12Q1/6806 ,  C12N15/1093 ,  C12Q1/6855 ,  C12Q2525/119 ,  C12Q2525/186

    摘要： The invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides and also the use of the 5′ and 3′ modified library of templates in methods of solid-phase nucleic acid amplification. In particular, the invention relates to a method of preparing a 5′ and 3′ modified library of template polynucleotides which have common sequences at their 5′ ends and at their 3′ ends, wherein over-representation of “end” sequences of the primary polynucleotide molecules from when the 5′ and 3′ modified library is generated is greatly reduced or prevented.

    摘要翻译： 本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，以及在固相核酸扩增方法中使用5'和3'修饰的模板文库。 特别地，本发明涉及一种制备模板多核苷酸的5'和3'修饰文库的方法，该文库在其5'末端和3'末端具有共同序列，其中主要“末端”序列的过表达 生成5'和3'修饰的文库时的多核苷酸分子大大减少或防止。