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74 条记录 (耗时 0.023 秒)

    Method for sequencing nucleic acids by observing the uptake of nucleotides modified with bulky groups
    11.
    发明申请
    Method for sequencing nucleic acids by observing the uptake of nucleotides modified with bulky groups 审中-公开
    通过观察用大体积改性的核苷酸摄取来测序核酸的方法

    公开(公告)号：US20070059733A1

    公开(公告)日：2007-03-15

    申请号：US11445884

    申请日：2006-06-02

    申请人： Narayanan Sundararajan ,  Andrew Berlin ,  Mineo Yamakawa ,  Xing Su ,  Selena Chan ,  Tae-Woong Koo

    发明人： Narayanan Sundararajan ,  Andrew Berlin ,  Mineo Yamakawa ,  Xing Su ,  Selena Chan ,  Tae-Woong Koo

    IPC分类号： C12Q1/68 ,  G06F19/00 ,  C12M3/00

    CPC分类号： C12Q1/6825 ,  B82Y5/00 ,  B82Y15/00 ,  B82Y30/00 ,  C12Q1/6869 ,  C12Q2563/155 ,  C12Q2565/607

    摘要： The present methods and apparatus concern nucleic acid sequencing by incorporation of nucleotides into nucleic acid strands. The incorporation of nucleotides is detected by changes in the mass and/or surface stress of the structure. In some embodiments of the invention, the structure comprises one or more nanoscale or microscale cantilevers. In certain embodiments of the invention, each different type of nucleotide is distinguishably labeled with a bulky group and each incorporated nucleotide is identified by the changes in mass and/or surface stress of the structure upon incorporation of the nucleotide. In alternative embodiments of the invention only one type of nucleotide is exposed at a time to the nucleic acids. Changes in the properties of the structure may be detected by a variety of methods, such as piezoelectric detection, shifts in resonant frequency of the structure, and/or position sensitive photodetection.

    摘要翻译： 本发明的方法和装置涉及通过将核苷酸掺入核酸链而进行的核酸测序。 通过结构的质量和/或表面应力的变化来检测核苷酸的掺入。 在本发明的一些实施例中，该结构包括一个或多个纳米级或微尺寸悬臂。 在本发明的某些实施方案中，每种不同类型的核苷酸可以用大体积区分地标记，并且每个掺入的核苷酸通过结合核苷酸时结构的质量和/或表面应力的变化来鉴定。 在本发明的替代实施方案中，一次仅将一种类型的核苷酸暴露于核酸。 可以通过各种方法来检测结构的性质的变化，例如压电检测，结构的谐振频率偏移和/或位置敏感的光电检测。

    Polymer sequencing using selectively labeled monomers and data integration
    14.
    发明申请
    Polymer sequencing using selectively labeled monomers and data integration 审中-公开
    使用选择性标记的单体和数据整合的聚合物测序

    公开(公告)号：US20050186576A1

    公开(公告)日：2005-08-25

    申请号：US10782014

    申请日：2004-02-19

    申请人： Selena Chan ,  Xing Su ,  Andrew Berlin ,  Tae-Woong Koo

    发明人： Selena Chan ,  Xing Su ,  Andrew Berlin ,  Tae-Woong Koo

    IPC分类号： B01L3/00 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50

    CPC分类号： B82Y5/00 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2565/629 ,  C12Q2527/113 ,  C12Q2521/319 ,  C12Q2565/631 ,  C12Q2565/601

    摘要： Methods and apparatuses for sequencing single polymer molecules, such as a nucleic acid strand, are discussed. A discussed method comprises dividing a polymer sample into a number of polymer subsamples equal to the number of different monomer types and partially labeling only one of the monomer types in each polymer subsample. The method may further comprise placing a subsample into a reaction chamber, sequentially separating each monomer from the polymer subsample, and detecting the labels of each separated labeled monomer as a function of time. The time between each labeled monomer may be used to construct a monomer-time map for each polymer sub-sample using overlapping data analysis and frequency analysis. Time maps may then be assembled/aligned into a polymer sequence from the monomer-time maps of each of the polymer subsamples using non-overlapping data analysis.

    摘要翻译： 讨论了用于测序单一聚合物分子（例如核酸链）的方法和装置。 所讨论的方法包括将聚合物样品分成等于不同单体类型的数量的多个聚合物子样品，并且在每个聚合物子样品中仅部分地标记单体类型之一。 该方法还可以包括将子样品放入反应室中，顺序地从聚合物子样品中分离每个单体，并检测每个分离的标记单体的标记作为时间的函数。 每个标记的单体之间的时间可以用于使用重叠的数据分析和频率分析来构建每个聚合物子样品的单体时间图。 然后可以使用不重叠的数据分析，从每个聚合物子样品的单体时间图，将时间图组装/排列成聚合物序列。

    Cellular analysis using Raman surface scanning
    15.
    发明授权
    Cellular analysis using Raman surface scanning 有权
    使用拉曼表面扫描的细胞分析

    公开(公告)号：US07776547B2

    公开(公告)日：2010-08-17

    申请号：US11027470

    申请日：2004-12-30

    申请人： Mark Roth ,  Andrew Berlin ,  Selena Chan ,  Tae-Woong Koo ,  Xing Su ,  Lei Sun

    发明人： Mark Roth ,  Andrew Berlin ,  Selena Chan ,  Tae-Woong Koo ,  Xing Su ,  Lei Sun

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54366 ,  G01N33/54373 ,  G01N33/58 ,  Y10T436/10

    摘要： Methods and apparatus are provided for assaying cell samples, which may be living cells, using probes labeled with composite organic-inorganic nanoparticles (COINs) and microspheres with COINs embedded within a polymer matrix to which the probe moiety is attached. COINs intrinsically produce SERS signals upon laser irradiation, making COIN-labeled probes particularly suitable in a variety of methods for assaying cells, including biological molecules that may be contained on or within cells, most of which are not inherently Raman-active. The invention provides variations of the sandwich immunoassay employing both specific and degenerate binding, methods for reverse phase assay of tissue samples and cell microstructures, in solution displacement and competition assays, and the like. Systems and chips useful for practicing the invention assays are also provided.

    摘要翻译： 提供了用于使用用复合有机 - 无机纳米颗粒（COIN）标记的探针和嵌入探针部分所连接的聚合物基质内的COIN的微球来测定可能为活细胞的细胞样品的方法和装置。 COIN在激光照射下固有地产生SERS信号，使得COIN标记的探针特别适用于多种测定细胞的方法，包括可能包含在细胞内或细胞内的生物分子，其中大部分不是固有的拉曼活性的。 本发明提供使用特异性和简并结合的夹心免疫测定的变体，组织样品和细胞微结构的反相测定方法，溶液置换和竞争测定等。 还提供了用于实施本发明测定的系统和芯片。

    Detection of biomolecules using porous biosensors and raman spectroscopy
    17.
    发明授权
    Detection of biomolecules using porous biosensors and raman spectroscopy 有权
    使用多孔生物传感器和拉曼光谱法检测生物分子

    公开(公告)号：US07271896B2

    公开(公告)日：2007-09-18

    申请号：US10748390

    申请日：2003-12-29

    申请人： Selena Chan ,  Tae-Woong Koo

    发明人： Selena Chan ,  Tae-Woong Koo

    IPC分类号： G01J3/44

    CPC分类号： G01N33/54373 ,  G01N21/6428 ,  G01N21/65 ,  G01N21/658

    摘要： The invention provides methods used to analyze the contents of a biological sample, such as blood serum, with cascade Raman sensing. A fluorescence producing nanoporous biosensor having probes that bind specifically to known analytes is contacted with a biological sample and one or more bound complexes coupled to the porous semiconductor structure are formed. The bound complexes are contacted with a Raman-active probe that binds specifically to the bound complexes and the biosensor is illuminated to generate fluorescent emissions from the biosensor. These fluorescent emissions generate Raman signals from the bound complexes. The Raman signals produced by the bound complexes are detected and the Raman signal associated with a bound protein-containing analyte is indicative of the presence of the protein-containing compound in the sample. The invention methods are useful to provide a protein profile of a patient sample. The invention also provides detection systems useful to practice the invention methods.

    摘要翻译： 本发明提供了利用级联拉曼检测来分析生物样品如血清的含量的方法。 具有特异性结合已知分析物的探针的产生荧光的纳米多孔生物传感器与生物样品接触，并形成耦合到多孔半导体结构的一个或多个结合复合物。 将结合的复合物与与结合的复合物特异性结合的拉曼活性探针接触，并且生物传感器被照射以产生来自生物传感器的荧光发射。 这些荧光发射从结合的复合物产生拉曼信号。 检测由结合的复合物产生的拉曼信号，并且与结合的含蛋白质的分析物相关的拉曼信号指示样品中含蛋白质的化合物的存在。 本发明方法可用于提供患者样品的蛋白质谱。 本发明还提供了用于实施本发明方法的检测系统。

    Detecting molecular binding by monitoring feedback controlled cantilever deflections
    19.
    发明申请
    Detecting molecular binding by monitoring feedback controlled cantilever deflections 有权
    通过监测反馈控制的悬臂偏转来检测分子结合

    公开(公告)号：US20050244820A1

    公开(公告)日：2005-11-03

    申请号：US11111308

    申请日：2005-04-20

    申请人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew Berlin

    发明人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew Berlin

    IPC分类号： C12Q1/68 ,  G01N33/543 ,  C12Q1/70 ,  C12Q1/04

    CPC分类号： C12Q1/6825 ,  B82Y5/00 ,  G01N33/54373 ,  G01N2800/52 ,  Y10S977/924 ,  C12Q2565/501

    摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

    摘要翻译： 本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。 在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。 将探针与分析物结合导致由检测单元检测到的悬臂的偏转。 可以应用平衡力将悬臂恢复到其原始位置。 平衡力可以是磁性的，电的或辐射的。 生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。 计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。 样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。