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    Methods and device for DNA sequencing using surface enhanced raman scattering (SERS)
    1.
    发明授权
    Methods and device for DNA sequencing using surface enhanced raman scattering (SERS) 失效
    使用表面增强拉曼散射（SERS）进行DNA测序的方法和装置

    公开(公告)号：US07476501B2

    公开(公告)日：2009-01-13

    申请号：US10108128

    申请日：2002-03-26

    申请人： Selena Chan ,  Xing Su ,  Tae-Woong Koo

    发明人： Selena Chan ,  Xing Su ,  Tae-Woong Koo

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  B01L3/5027 ,  C12Q2565/60 ,  C12Q2565/631 ,  C12Q2565/632 ,  G01N21/658 ,  G01N33/587 ,  G01N2021/653 ,  G01N2021/656

    摘要： The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, exonuclease treatment of the nucleic acids 109 results in the release of nucleotides 110. The nucleotides may pass from a reaction chamber 101 through a microfluidic channel 102 and enter a nanochannel or microchannel 103. The nanochannel or microchannel 103 may be packed with nanoparticle 111 aggregates containing hot spots for Raman detection. As the nucleotides 110 pass through the nanoparticle 111 hot spots, they may be detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Identification of the sequence of nucleotides 110 released from the nucleic acid 109 provides the nucleic acid sequence. Other embodiments of the invention concern apparatus 100 for nucleic acid sequencing.

    摘要翻译： 本文公开的方法和装置涉及通过增强拉曼光谱进行的核酸测序。 在本发明的某些实施方案中，核酸109的核酸外切酶处理导致核苷酸110的释放。核苷酸可以从反应室101通过微流体通道102并进入纳米通道或微通道103.纳米通道或微通道103可以 填充含有用于拉曼检测的热点的纳米颗粒111聚集体。 当核苷酸110通过纳米颗粒111热点时，它们可以通过表面增强拉曼光谱（SERS），表面增强共振拉曼光谱（SERRS）和/或相干抗斯托克斯拉曼光谱（CARS）来检测。 从核酸109释放的核苷酸序列110的鉴定提供了核酸序列。 本发明的其它实施方案涉及用于核酸测序的设备100。

    Detecting molecular binding by monitoring feedback controlled cantilever deflections
    2.
    发明授权
    Detecting molecular binding by monitoring feedback controlled cantilever deflections 失效
    通过监测反馈控制的悬臂偏转来检测分子结合

    公开(公告)号：US07105301B2

    公开(公告)日：2006-09-12

    申请号：US10667776

    申请日：2003-09-22

    申请人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

    发明人： Xing Su ,  Selena Chan ,  Tae-Woong Koo ,  Mineo Yamakawa ,  Andrew A. Berlin

    IPC分类号： C12Q1/68 ,  C12Q1/70 ,  G01N33/566 ,  G01N33/563

    CPC分类号： C12Q1/6825 ,  B82Y5/00 ,  G01N33/54373 ,  G01N2800/52 ,  Y10S977/924 ,  C12Q2565/501

    摘要： The present methods and apparatus concern the detection and/or identification of target analytes using probe molecules. In various embodiments of the invention, the probes or analytes are attached to one or more cantilevers. Binding of a probe to an analyte results in deflection of the cantilever, detected by a detection unit. A counterbalancing force may be applied to restore the cantilever to its original position. The counterbalancing force may be magnetic, electrical or radiative. The detection unit and the mechanism generating the counterbalancing force may be operably coupled to an information processing and control unit, such as a computer. The computer may regulate a feedback loop that maintains the cantilever in a fixed position by balancing the deflecting force and the counterbalancing force. The concentration of analytes in a sample may be determined from the magnitude of the counterbalancing force required to maintain the cantilever in a fixed position.

    摘要翻译： 本方法和装置涉及使用探针分子检测和/或鉴定目标分析物。 在本发明的各种实施方案中，探针或分析物附着到一个或多个悬臂。 将探针与分析物结合导致由检测单元检测到的悬臂的偏转。 可以应用平衡力将悬臂恢复到其原始位置。 平衡力可以是磁性的，电的或辐射的。 生成平衡力的检测单元和机构可以可操作地耦合到诸如计算机的信息处理和控制单元。 计算机可以通过平衡偏转力和平衡力来调节将悬臂维持在固定位置的反馈回路。 样品中分析物的浓度可以从将悬臂维持在固定位置所需的平衡力的大小来确定。

    Chemical enhancement in surface enhanced raman scattering using lithium salts
    4.
    发明授权
    Chemical enhancement in surface enhanced raman scattering using lithium salts 失效
    使用锂盐的表面增强拉曼散射的化学增强

    公开(公告)号：US07019828B2

    公开(公告)日：2006-03-28

    申请号：US10387080

    申请日：2003-03-12

    申请人： Xing Su ,  Lei Sun ,  Tae-Woong Koo ,  Selena Chan

    发明人： Xing Su ,  Lei Sun ,  Tae-Woong Koo ,  Selena Chan

    IPC分类号： G01J3/44

    CPC分类号： G01N21/658 ,  B82Y10/00 ,  B82Y20/00

    摘要： Briefly, in accordance with one embodiment of the invention, the intensity of the signals from surface enhanced Raman spectroscopy may be increased by using lithium chloride as an enhancer to activate a metallic structure used for surface enhanced Raman spectroscopy. The increased signal intensity may allow surface enhanced Raman spectroscopy to be utilized to detect individual analytes such as nucleotides, for example in DNA sequencing without requiring a dye or radioactive label.

    摘要翻译： 简而言之，根据本发明的一个实施例，可以通过使用氯化锂作为增强剂激活用于表面增强拉曼光谱的金属结构来增加来自表面增强拉曼光谱的信号的强度。 增加的信号强度可以允许使用表面增强拉曼光谱来检测单个分析物例如核苷酸，例如在DNA测序中，而不需要染料或放射性标记。

    Method to detect molecular binding by surface-enhanced Raman spectroscopy
    5.
    发明申请
    Method to detect molecular binding by surface-enhanced Raman spectroscopy 审中-公开
    通过表面增强拉曼光谱法检测分子结合的方法

    公开(公告)号：US20050221507A1

    公开(公告)日：2005-10-06

    申请号：US10814981

    申请日：2004-03-30

    申请人： Tae-Woong Koo ,  Selena Chan

    发明人： Tae-Woong Koo ,  Selena Chan

    IPC分类号： G01N21/65 ,  G01N33/543 ,  G01N33/553

    CPC分类号： G01N33/553 ,  G01N21/658 ,  G01N33/54373

    摘要： Provided herein are methods for detecting molecular binding using surface enhanced Raman spectroscopy (SERS). The SERS signal can be generated by associating one of the binding partners with a SERS-active particle or substrate. Binding is detected by detecting a change in a SERS signal after two binding partners are contacted with each other as compared to before the binding partners are contacted with each other. The method is useful for detecting binding of biomolecules such as antibodies to antigens and receptors to ligands.

    摘要翻译： 本文提供了使用表面增强拉曼光谱（SERS）检测分子结合的方法。 SERS信号可以通过将一个结合配偶体与SERS活性颗粒或底物相关联而产生。 通过检测两个结合配偶体彼此接触后的SERS信号的变化来检测结合，与结合配偶体彼此接触之前相比。 该方法可用于检测生物分子如抗体与抗体和受体与配体的结合。

    Detection of biomolecules using porous biosensors and Raman spectroscopy
    7.
    发明授权
    Detection of biomolecules using porous biosensors and Raman spectroscopy 有权
    使用多孔生物传感器和拉曼光谱法检测生物分子

    公开(公告)号：US07843562B2

    公开(公告)日：2010-11-30

    申请号：US11700919

    申请日：2007-02-01

    申请人： Selena Chan ,  Tae-Woong Koo

    发明人： Selena Chan ,  Tae-Woong Koo

    IPC分类号： G01J3/44

    CPC分类号： G01N33/54373 ,  G01N21/6428 ,  G01N21/65 ,  G01N21/658

    摘要： The invention provides methods used to analyze the contents of a biological sample, such as blood serum, with cascade Raman sensing. A fluorescence producing nanoporous biosensor having probes that bind specifically to known analytes is contacted with a biological sample and one or more bound complexes coupled to the porous semiconductor structure are formed. The bound complexes are contacted with a Raman-active probe that binds specifically to the bound complexes and the biosensor is illuminated to generate fluorescent emissions from the biosensor. These fluorescent emissions generate Raman signals from the bound complexes. The Raman signals produced by the bound complexes are detected and the Raman signal associated with a bound protein-containing analyte is indicative of the presence of the protein-containing compound in the sample. The invention methods are useful to provide a protein profile of a patient sample. The invention also provides detection systems useful to practice the invention methods.

    摘要翻译： 本发明提供了利用级联拉曼检测来分析生物样品如血清的含量的方法。 具有特异性结合已知分析物的探针的产生荧光的纳米多孔生物传感器与生物样品接触，并形成耦合到多孔半导体结构的一个或多个结合复合物。 将结合的复合物与与结合的复合物特异性结合的拉曼活性探针接触，并且生物传感器被照射以产生来自生物传感器的荧光发射。 这些荧光发射从结合的复合物产生拉曼信号。 检测由结合的复合物产生的拉曼信号，并且与结合的含蛋白质的分析物相关的拉曼信号指示样品中含蛋白质的化合物的存在。 本发明方法可用于提供患者样品的蛋白质谱。 本发明还提供了用于实施本发明方法的检测系统。

    Detection of biomolecules using porous biosensors and Raman spectroscopy
    8.
    发明申请
    Detection of biomolecules using porous biosensors and Raman spectroscopy 有权
    使用多孔生物传感器和拉曼光谱法检测生物分子

    公开(公告)号：US20080108131A1

    公开(公告)日：2008-05-08

    申请号：US11700919

    申请日：2007-02-01

    申请人： Selena Chan ,  Tae-Woong Koo

    发明人： Selena Chan ,  Tae-Woong Koo

    IPC分类号： C12M1/34 ,  G01N21/00

    CPC分类号： G01N33/54373 ,  G01N21/6428 ,  G01N21/65 ,  G01N21/658

    摘要： The invention provides methods used to analyze the contents of a biological sample, such as blood serum, with cascade Raman sensing. A fluorescence producing nanoporous biosensor having probes that bind specifically to known analytes is contacted with a biological sample and one or more bound complexes coupled to the porous semiconductor structure are formed. The bound complexes are contacted with a Raman-active probe that binds specifically to the bound complexes and the biosensor is illuminated to generate fluorescent emissions from the biosensor. These fluorescent emissions generate Raman signals from the bound complexes. The Raman signals produced by the bound complexes are detected and the Raman signal associated with a bound protein-containing analyte is indicative of the presence of the protein-containing compound in the sample. The invention methods are useful to provide a protein profile of a patient sample. The invention also provides detection systems useful to practice the invention methods.

    摘要翻译： 本发明提供了利用级联拉曼检测来分析生物样品如血清的含量的方法。 具有特异性结合已知分析物的探针的产生荧光的纳米多孔生物传感器与生物样品接触，并形成耦合到多孔半导体结构的一个或多个结合复合物。 将结合的复合物与与结合的复合物特异性结合的拉曼活性探针接触，并且生物传感器被照射以产生来自生物传感器的荧光发射。 这些荧光发射从结合的复合物产生拉曼信号。 检测由结合的复合物产生的拉曼信号，并且与结合含蛋白质的分析物相关的拉曼信号指示样品中含蛋白质的化合物的存在。 本发明方法可用于提供患者样品的蛋白质谱。 本发明还提供了用于实施本发明方法的检测系统。