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    METHOD AND REAGENT FOR MEASURING MEVALONIC ACID, 3-HYDROXYMETHYLGLUTARYL COENZYME A, AND COENZYME A
    11.
    发明申请
    METHOD AND REAGENT FOR MEASURING MEVALONIC ACID, 3-HYDROXYMETHYLGLUTARYL COENZYME A, AND COENZYME A 审中-公开
    用于测量甲酸，3-羟基甲基胆碱酯酶A和COENZYME A的方法和试剂

    公开(公告)号：US20120040387A1

    公开(公告)日：2012-02-16

    申请号：US13144710

    申请日：2009-01-19

    申请人： Takeshi Matsuoka

    发明人： Takeshi Matsuoka

    IPC分类号： C12Q1/26 ,  C07C59/105 ,  C12N9/04 ,  C12Q1/48 ,  C12N1/20

    CPC分类号： C12Q1/008 ,  C12N9/0006 ,  C12N9/001 ,  C12N9/1205 ,  C12Q1/26 ,  C12R1/01 ,  C12R1/38 ,  G01N33/5308 ,  G01N2333/904

    摘要： The present invention provides a method for measuring the concentration of an analyte in a test solution wherein the analyte is mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A, comprising the following steps (p) and (q): (p) a step of allowing an enzyme that catalyzes a reaction represented by Reaction Formula 1 and an enzyme that catalyzes a reaction represented by Reaction Formula 2 to act on a test solution containing mevalonic acid and/or 3-hydroxymethylglutaryl coenzyme A in the presence of a hydrogen acceptor X, a hydrogen donor Y, and coenzyme A; and (q) a step of measuring an amount of: a reduced hydrogen acceptor X that is produced; or an oxidized hydrogen donor Y that is produced; or a hydrogen acceptor X that is decreased; or a hydrogen donor Y that is decreased, wherein the hydrogen donor Y and the reduced hydrogen acceptor X are not the same.

    摘要翻译： 本发明提供了一种用于测量测试溶液中分析物浓度的方法，其中分析物是甲羟戊酸和/或3-羟甲基戊二酰辅酶A，包括以下步骤（p）和（q）：（p）步骤 允许催化由反应式1表示的反应的酶和催化反应式2表示的反应的酶在氢受体X存在下作用于含有甲羟戊酸和/或3-羟甲基戊二酰辅酶A的试验溶液， 氢供体Y和辅酶A; 和（q）测量生成的还原氢受体X的量的步骤; 或产生的氧化氢供体Y; 或降低的氢受体X; 或降低的氢供体Y，其中氢供体Y和还原氢受体X不相同。

    Method for screening a protease
    16.
    发明授权
    Method for screening a protease 有权
    筛选蛋白酶的方法

    公开(公告)号：US07943337B2

    公开(公告)日：2011-05-17

    申请号：US12506695

    申请日：2009-07-21

    申请人： Takeshi Matsuoka ,  Shinji Koga ,  Takuji Kouzuma

    发明人： Takeshi Matsuoka ,  Shinji Koga ,  Takuji Kouzuma

    IPC分类号： C12Q1/26 ,  C12Q1/37

    CPC分类号： C12N9/0004 ,  C12N9/50 ,  C12Q1/37 ,  C12R1/01 ,  C12R1/07 ,  G01N33/66 ,  G01N33/723 ,  G01N2333/95

    摘要： There is provided a method for specifically determining a glycated β-chain N-terminal of glycated hemoglobin using enzymes without a separation operation, and a determination reagent kit therefor. A protease that cleaves a glycated amino acid and/or a glycated peptide from a glycated β-chain N-terminal without substantially cleaving a glycated amino acid or a glycated peptide from a glycated α-chain N-terminal of glycated hemoglobin or a fragment thereof is screened. The method of specifically determining a glycated β-chain N-terminal of glycated hemoglobin and the determination reagent kit are provided by using the protease obtained by the screening method. According to the present invention, a glycated β-chain N-terminal of glycated hemoglobin can specifically be determined without a separation operation.

    摘要翻译： 提供了使用不分离操作的酶来特异性测定糖化血红蛋白的糖基化和N-末端的N-末端的方法及其测定试剂盒。 一种蛋白酶，其从糖基化连接的N-末端切割糖基化氨基酸和/或糖化肽，而不从糖化血红蛋白的糖基化α-链N-末端切割糖基化氨基酸或糖化肽 被筛选。 通过使用通过筛选方法获得的蛋白酶提供了特异性测定糖化血红蛋白的糖基化和连结的N-末端的方法和测定试剂盒。 根据本发明，可以在没有分离操作的情况下，具体地确定糖化血红蛋白的糖基化和连结的N-末端。

    PORTABLE FASTENING TOOL
    17.
    发明申请
    PORTABLE FASTENING TOOL 有权
    便携式工具

    公开(公告)号：US20090266863A1

    公开(公告)日：2009-10-29

    申请号：US12088524

    申请日：2006-09-01

    申请人： Hideki Ishida ,  Hitoshi Tsuzuki ,  Takeshi Matsuoka

    发明人： Hideki Ishida ,  Hitoshi Tsuzuki ,  Takeshi Matsuoka

    IPC分类号： B25C1/06

    CPC分类号： B25C1/06

    摘要： To provide a compact portable fastening tool which can be easily pushed at its handle portion onto a punched side, even in case a magazine is attached at an inclination with respect to the horizon, so that it can be easily used even in a narrow place. An electric fastening tool 1 comprises: a housing 2 having a handle portion 2B formed to extend from a trunk portion 2A; an ejection unit attached to the lower portion of the housing 2; a magazine 5 attached to the ejection unit; a motor housed in the housing 2; a battery pack 3 for driving the motor; a flywheel rotationally driven by the motor; a follower shaft selectively rotated by the kinetic energy of the flywheel; and a plunger adapted to be linearly moved in the housing 2 by the rotation of the follower shaft thereby to drive the nail fed into the ejection unit. The magazine 5 is so attached that it is inclined in a side view with respect to the trunk portion 2A of the housing 2 and that it is inclined in a bottom view with respect to the handle portion 2B of the housing 2.

    摘要翻译： 为了提供一种紧凑的便携式紧固工具，即使在相对于水平线倾斜地安装有盒子的情况下，也可以将其在其手柄部分容易地推到冲压侧，使得即使在狭窄的位置也可以容易地使用。 电动紧固工具1包括：壳体2，其具有形成为从主体部分2A延伸的手柄部分2B; 附接到壳体2的下部的喷射单元; 安装在喷射单元上的暗盒5; 容纳在壳体2中的电动机; 用于驱动电动机的电池组3; 由电机旋转驱动的飞轮; 选择性地由飞轮的动能旋转的从动轴; 以及适于通过从动轴的旋转在壳体2中直线移动的柱塞，从而驱动送入喷射单元的指甲。 杂志5被安装成相对于壳体2的主体部分2A的侧视图倾斜，并且相对于壳体2的把手部分2B在底视图中倾斜。

    Power tool
    18.
    发明授权
    Power tool 有权
    电动工具

    公开(公告)号：US07490746B2

    公开(公告)日：2009-02-17

    申请号：US11603142

    申请日：2006-11-22

    申请人： Masato Sakai ,  Takeshi Matsuoka ,  Chikai Yoshimizu ,  Masanori Watanabe ,  Katsuhiro Oomori ,  Takuya Teranishi

    发明人： Masato Sakai ,  Takeshi Matsuoka ,  Chikai Yoshimizu ,  Masanori Watanabe ,  Katsuhiro Oomori ,  Takuya Teranishi

    IPC分类号： A45F5/00

    CPC分类号： B25B23/045 ,  B25F5/029 ,  B25H3/006 ,  Y10S224/904 ,  Y10T408/96

    摘要： A power tool includes a hook portion having a hook holding portion, which is provided in a housing and has engaging teeth provided in the housing. A hook having a shaft portion is inserted into the hook holding portion and provided with fitting teeth meshing with the engaging teeth An elastic body is adapted to push the hook against a handle portion at all times, and a slip-off preventing part holding portion is adapted to move the hook in a direction of an axis thereof against a force of the elastic body and to cancel the mesh between the teeth to thereby enable the hook to turn.

    摘要翻译： 电动工具包括具有钩保持部分的钩部分，该钩部分设置在壳体中并且具有设置在壳体中的接合齿。 具有轴部的钩子被插入到钩保持部分中，并且设置有与接合齿啮合的配合齿。弹性体适于一直将钩推在手柄部分上，并且防脱部分保持部分是 适于使钩沿其轴线的方向抵抗弹性体的力移动并抵消齿之间的网孔，从而使钩能够转动。