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公开(公告)号：US07666593B2
公开(公告)日：2010-02-23
申请号：US11213621
申请日：2005-08-26
Applicant: Stanley Lapidus
Inventor： Stanley Lapidus
IPC: C12Q1/68
CPC classification number: C12Q1/68 , C12Q1/6869 , C12Q1/6874 , C12Q2565/518 , C12Q2563/107 , C12Q2533/101
Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.
Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。
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公开(公告)号：US20090191565A1
公开(公告)日：2009-07-30
申请号：US12371310
申请日：2009-02-13
Applicant: Stanley Lapidus , Phillip Richard Buzby , Timothy Harris
Inventor： Stanley Lapidus , Phillip Richard Buzby , Timothy Harris
IPC: C12Q1/68
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2527/113
Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or fill completion.
Abstract translation: 本发明提供了对多核苷酸进行测序的方法，包括在反应已经运行到接近或完成之前通过合成反应在序列中停止延伸循环。
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公开(公告)号：US20060024711A1
公开(公告)日：2006-02-02
申请号：US11167046
申请日：2005-06-23
Applicant: Stanley Lapidus , Philip Buzby
Inventor： Stanley Lapidus , Philip Buzby
CPC classification number: C12Q1/6806 , C12Q1/6869 , C12Q2565/501 , C12Q2531/125
Abstract: The invention provides methods for sequencing a nucleic acid comprising conducting rolling circle amplification on a circular nucleic acid template, wherein the resulting amplicon is optionally anchored to a substrate in an individually optically resolvable manner, and performing a sequencing reaction.
Abstract translation: 本发明提供了用于对核酸进行测序的方法，包括在环状核酸模板上进行滚环扩增，其中所得扩增子任选地以单独光学可分辨的方式锚定于底物，并进行测序反应。
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公开(公告)号：US20050260609A1
公开(公告)日：2005-11-24
申请号：US10852028
申请日：2004-05-24
Applicant: Stanley Lapidus
Inventor： Stanley Lapidus
CPC classification number: C12Q1/6869
Abstract: The invention provides methods and devices for high throughput single molecule sequencing of a plurality of target nucleic acids using a universal primer. Devices of the invention comprise a plurality of oligonucleotides, each having the same sequence, bound to a solid support, and ligated to a plurality of target nucleic acids.
Abstract translation: 本发明提供使用通用引物对多个靶核酸进行高通量单分子测序的方法和装置。 本发明的装置包含多个寡核苷酸，每个具有相同的序列，与固体支持物结合，并连接到多个靶核酸。
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公开(公告)号：US09012144B2
公开(公告)日：2015-04-21
申请号：US13008182
申请日：2011-01-18
Applicant: Stanley Lapidus , Philip Richard Buzby , Timothy Harris
Inventor： Stanley Lapidus , Philip Richard Buzby , Timothy Harris
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2527/113
Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.
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Patent Agency Ranking
公开(公告)号：US07897345B2
公开(公告)日：2011-03-01
申请号：US12371310
申请日：2009-02-13
Applicant: Stanley Lapidus , Phillip Richard Buzby , Timothy Harris
Inventor： Stanley Lapidus , Phillip Richard Buzby , Timothy Harris
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2527/113
Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or fill completion.
Abstract translation: 本发明提供了对多核苷酸进行测序的方法，包括在反应已经运行到接近或完成之前通过合成反应在序列中停止延伸循环。
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公开(公告)号：US20100143932A1
公开(公告)日：2010-06-10
申请号：US12708153
申请日：2010-02-18
Applicant: Stanley Lapidus
Inventor： Stanley Lapidus
IPC: C12Q1/68
CPC classification number: C12Q1/68 , C12Q1/6869 , C12Q1/6874 , C12Q2565/518 , C12Q2563/107 , C12Q2533/101
Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.
Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。
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公开(公告)号：US20070122828A1
公开(公告)日：2007-05-31
申请号：US11588108
申请日：2006-10-26
Applicant: Stanley Lapidus , Phillip Buzby , Timothy Harris
Inventor： Stanley Lapidus , Phillip Buzby , Timothy Harris
CPC classification number: C12Q1/6874 , C12Q1/6869 , C12Q2527/113
Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.
Abstract translation: 本发明提供了用于测序多核苷酸的方法，包括在反应已经运行到接近或完全完成之前通过合成反应在序列中停止延伸循环。
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19.发明申请公开(公告)号：US20070070349A1
公开(公告)日：2007-03-29
申请号：US11234420
申请日：2005-09-23
Applicant: Timothy Harris , Philip Buzby , Mirna Jarosz , Jaime Gill , Howard Weiss , Stanley Lapidus
Inventor： Timothy Harris , Philip Buzby , Mirna Jarosz , Jaime Gill , Howard Weiss , Stanley Lapidus
IPC: G01N21/64
CPC classification number: G01N21/645 , G01J3/10 , G01J3/36 , G01J3/4406 , G01N21/552 , G01N21/6428 , G01N21/6456 , G01N2021/6419 , G01N2021/6421 , G02B21/16 , G02B2207/113
Abstract: In one aspect the invention relates to an apparatus for analyzing the presence of a single molecule using total internal reflection. In one embodiment an apparatus for single molecule analysis includes a support having a sample located thereon; two sources of light at distinct wavelengths, a collimator for directing the light onto the sample through a total internal reflection objective; a receiver for receiving a fluorescent emission produced by a single molecule in the sample in response to the light; and a detector for detecting each of the wavelengths in the fluorescent emission. In another embodiment the apparatus further comprises a focusing laser for maintaining focus of the objective on the sample.
Abstract translation: 一方面，本发明涉及一种使用全内反射来分析单个分子的存在的装置。 在一个实施方案中，用于单分子分析的装置包括具有位于其上的样品的载体; 两个不同波长的光源，用于通过全内反射物镜将光引导到样品上的准直器; 接收器，用于响应于光接收由样品中的单个分子产生的荧光发射; 以及用于检测荧光发射中的每个波长的检测器。 在另一个实施例中，该装置还包括聚焦激光器，用于保持物体在样本上的焦点。
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公开(公告)号：US20070048744A1
公开(公告)日：2007-03-01
申请号：US11213621
申请日：2005-08-26
Applicant: Stanley Lapidus
Inventor： Stanley Lapidus
CPC classification number: C12Q1/68 , C12Q1/6869 , C12Q1/6874 , C12Q2565/518 , C12Q2563/107 , C12Q2533/101
Abstract: The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques.
Abstract translation: 本发明提供了使用固定的捕获探针和单分子测序技术来检测，列举或鉴定靶核酸分子的方法和装置。
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