公开(公告)号：US09322511B2

公开(公告)日：2016-04-26

申请号：US13627593

申请日：2012-09-26

Applicant: Stokes BIO Limited

Inventor： Mark Davies ,  Tara Dalton ,  Kieran Curran

IPC: G01N1/10 ,  F17D1/08 ,  B01F13/00 ,  B01F15/02 ,  B01L3/00 ,  B01L7/00

CPC classification number: B01L3/502784 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01F2215/0037 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L7/525 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2300/0838 ,  B01L2400/0478 ,  B01L2400/0487 ,  B01L2400/0633 ,  B01L2400/082 ,  C12Q1/686 ,  F17D1/08 ,  Y10T137/87676 ,  Y10T436/117497 ,  Y10T436/118339 ,  Y10T436/2575

Abstract: A bridge comprises a first inlet port, a second inlet port, an outlet port, and a chamber for silicone oil. The oil is density-matched with the reactor droplets such that a neutrally buoyant environment is created within the chamber. The oil within the chamber is continuously replenished by the oil separating the reactor droplets. This causes the droplets to assume a stable capillary-suspended spherical form upon entering the chamber. The spherical shape grows until large enough to span the gap between the ports, forming an axisymmetric liquid bridge. The introduction of a second droplet from the second inlet port causes the formation of an unstable funicular bridge that quickly ruptures from the, finer, second inlet port, and the droplets combine at the liquid bridge. In another embodiment, a droplet segments into smaller droplets which bridge the gap between the inlet and outlet ports.

Abstract translation: 桥梁包括第一入口端口，第二入口端口，出口端口和用于硅油的室。 油与反应器液滴密度匹配，使得在室内产生中性浮力的环境。 室内的油通过分离反应器液滴的油不断补充。 这使得液滴在进入室时呈现稳定的毛细管悬浮球形。 球形增长直到足够大以跨越端口之间的间隙，形成轴对称液桥。 从第二入口引入第二液滴导致不稳定的索道桥的形成，其从较细的第二入口快速破裂，并且液滴在液桥处结合。 在另一个实施例中，液滴分段成较小的液滴，桥接入口和出口之间的间隙。

公开(公告)号：US20150352513A1

公开(公告)日：2015-12-10

申请号：US14828336

申请日：2015-08-17

Applicant: STOKES BIO LIMITED

Inventor： Mark Davies ,  Tara Dalton ,  Julie Garvey ,  Kieran Curran ,  Damian Curtin

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01F13/0071 ,  B01F15/0201 ,  B01F15/0232 ,  B01J2219/00313 ,  B01J2219/00353 ,  B01J2219/00479 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01L3/5025 ,  B01L3/502784 ,  B01L2200/0673 ,  B01L2300/0829 ,  B01L2400/0487 ,  Y10T436/25

Abstract: A multi-port liquid bridge (1) adds aqueous phase droplets (10) in an enveloping oil phase carrier liquid (11) to a draft channel (4, 6). A chamber (3) links four ports, and it is permanently full of oil (11) when in use. Oil phase is fed in a draft flow from an inlet port (4) and exits through a draft exit port (6) and a compensating flow port (7). The oil carrier and the sample droplets (3) (“aqueous phase”) flow through the inlet port (5) with an equivalent fluid flow subtracted through the compensating port (7). The ports of the bridge (1) are formed by the ends of capillaries help in position in plastics housings. The phases are density matched to create an environment where gravitational forces are negligible. This results in droplets (10) adopting spherical forms when suspended from capillary tube tips. Furthermore, the equality of mass flow is equal to the equality of volume flow. The phase of the inlet flow from the droplet inlet port (5) and the draft inlet port (4) is used to determine the outlet port (6) flow phase.

Abstract translation: 多端口液体桥（1）将包含油相载体液体（11）中的水相液滴（10）加入到通风道（4,6）。 室（3）连接四个端口，在使用时永久充满油（11）。 油相在进气口（4）的进料流中进料，并通过排气口（6）和补偿流通口（7）排出。 油载体和样品液滴（“水相”）以相当的流体流通过入口端口（5）流过补偿口（7）。 桥（1）的端口由毛细管的端部形成在塑料外壳中的位置。 这些阶段是密度匹配的，以创造引力几乎可忽略的环境。 这导致当从毛细管尖端悬浮时，液滴（10）采用球形。 此外，质量流量的相等性等于体积流量的相等。 来自液滴入口（5）和进气口（4）的入口流的相位用于确定出口（6）流动相。