公开(公告)号：US20230287356A1

公开(公告)日：2023-09-14

申请号：US18319691

申请日：2023-05-18

Applicant: TOPPAN Inc.

Inventor： Shiro KITANO ,  Kei TSUKAMOTO

IPC: C12N5/071 ,  C12N5/077

CPC classification number: C12N5/069 ,  C12N5/0656 ,  C12N2513/00 ,  C12N2533/56 ,  C12N2533/54 ,  C12N2501/91

Abstract: A method of producing a three-dimensional cell tissue include obtaining a mixture including cells, a cationic substance, an extracellular matrix component and a polyelectrolyte, gelling the mixture such that a gel composition including the cells, cationic substance, extracellular matrix component and polyelectrolyte is obtained; and incubating the gel composition such that a three-dimensional cell tissue is obtained.

公开(公告)号：US20230203452A1

公开(公告)日：2023-06-29

申请号：US18171832

申请日：2023-02-21

Applicant: TOPPAN Inc.

Inventor： Kei TSUKAMOTO ,  Shiro KITANO ,  Tomoko KUNITOMI

IPC: C12N5/09

CPC classification number: C12N5/0693 ,  C12N2502/28 ,  C12N2502/1323 ,  C12N2513/00 ,  C12N2533/90

Abstract: A method of producing a conditioned medium for culturing a patient-derived cancer cell, including culturing, in a first medium for 24 hours or longer, a three-dimensional cell tissue including an extracellular matrix component, a polymer electrolyte, and a cell cluster including a fibroblast, and collecting the first medium in which the three-dimensional cell tissue has been cultured as the conditioned medium.

公开(公告)号：US20230201426A1

公开(公告)日：2023-06-29

申请号：US17926924

申请日：2021-06-16

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Shiro KITANO ,  Michiya MATSUSAKI ,  Hidehiro SAKURAI ,  Yuta UETAKE

IPC: A61L27/52 ,  A61L27/04 ,  A61L27/24 ,  A61L27/36

CPC classification number: A61L27/52 ,  A61L27/047 ,  A61L27/24 ,  A61L27/3633

Abstract: The present invention relates to a gel composition containing at least one selected from the group consisting of an extracellular matrix component and a fragmented extracellular matrix component, and an ion of a metal element.

公开(公告)号：US20230110381A1

公开(公告)日：2023-04-13

申请号：US17908283

申请日：2021-03-04

Applicant: OSAKA UNIVERSITY ,  TOPPAN INC.

Inventor： Michiya MATSUSAKI ,  Naoko SASAKI ,  Shiro KITANO ,  Shinji IRIE

IPC: A61L27/50 ,  A61L27/36 ,  A61L27/24 ,  A61L27/38 ,  C12N5/00

Abstract: Disclosed is a method for controlling the Young’s modulus of a three-dimensional tissue containing cells and an extracellular matrix by adjusting the average diameter of an extracellular matrix in production of the three-dimensional tissue.

公开(公告)号：US20220341918A1

公开(公告)日：2022-10-27

申请号：US17642608

申请日：2020-08-27

Applicant: TOPPAN INC. ,  Osaka University

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI ,  Naoko SASAKI ,  Atsuo AMANO ,  Hiroki TAKEUCHI

IPC: G01N33/50 ,  C12N5/071

Abstract: Provided is a cell structure including: a connective tissue structure; and an epithelial structure placed on the connective tissue structure, in which the connective tissue structure contains a fragmented extracellular matrix component and first cells including mesenchymal cells, at least a part of the fragmented extracellular matrix component is placed between the first cells, and the epithelial structure contains epithelial cells.

公开(公告)号：US20220170911A1

公开(公告)日：2022-06-02

申请号：US17600348

申请日：2020-03-19

Applicant: TOPPAN INC. ,  OSAKA UNIVERSITY ,  Kyoto Prefectural Public University Corporation

Inventor： Shiro KITANO ,  Shinji IRIE ,  Michiya MATSUSAKI ,  Fiona LOUIS ,  Yoshihiro SOWA

IPC: G01N33/50 ,  C12N5/077 ,  C12N5/0775 ,  C12N5/071 ,  A61K35/35

Abstract: Disclosed is a cell structure comprising: a fragmented extracellular matrix component; and cells, wherein the cell structure comprises an intercellular vascular network, and the cells comprise at least adipocytes and vascular endothelial cells.