公开(公告)号：US07675676B2

公开(公告)日：2010-03-09

申请号：US11677444

申请日：2007-02-21

申请人： Tatsuo Nakata

发明人： Tatsuo Nakata

IPC分类号： G02B21/06 ,  G02B21/00

CPC分类号： G02B21/06 ,  G02B21/0052 ,  G02B21/0072 ,  G02B21/0076 ,  G02B21/008 ,  G02B21/16 ,  G02B21/24 ,  G02B21/32

摘要： A confocal microscope apparatus comprises a first optical scanning system which obtains a scan image of a sample using a laser beam from a first laser light source, a second optical scanning system which scans specific regions of a sample with a laser beam from a second laser light source that is different from the first laser light source, thereby causing a particular phenomenon, and a beam diameter varying mechanism which can change the beam diameter of the laser beam of at least one of the first optical scanning system and the second optical scanning system. With this configuration, the apparatus further comprises an excitation light intensity distribution calculator which calculates and stores the excitation light intensity distribution along a depth direction on the sample surface from the beam diameter of the laser beam output from the beam diameter varying mechanism.

摘要翻译： 共聚焦显微镜装置包括：第一光学扫描系统，其使用来自第一激光光源的激光束获得样品的扫描图像;第二光学扫描系统，其利用来自第二激光的激光束扫描样品的特定区域 源，其不同于第一激光光源，从而引起特定现象，以及光束直径改变机构，其可以改变第一光学扫描系统和第二光学扫描系统中的至少一个的激光束的光束直径。 利用该结构，该装置还包括：激发光强度分布计算器，其根据从光束直径变化机构输出的激光束的光束直径，沿着深度方向在样品表面上计算和存储激发光强度分布。

公开(公告)号：US20080246839A1

公开(公告)日：2008-10-09

申请号：US12059043

申请日：2008-03-31

申请人： Toshiyuki Hattori ,  Tatsuo Nakata ,  Yasunari Matsukawa ,  Akinori Araya ,  Masaharu Tomioka

发明人： Toshiyuki Hattori ,  Tatsuo Nakata ,  Yasunari Matsukawa ,  Akinori Araya ,  Masaharu Tomioka

IPC分类号： H04N7/18

CPC分类号： G02B21/367

摘要： It is possible to check for observation success or failure and the observation history without waiting for observation to be completely finished, thus saving time and energy required for observation, and avoiding lost opportunities for observation of precious samples etc. Provided is a microscope apparatus including an image acquisition unit for acquiring a plurality of frame images while varying a plurality of parameters; an image saving unit for successively saving the frame images acquired by the image acquisition unit; a property-information saving unit for saving property information in which identifying information of the saved frame images is associated with the parameters; and a control unit for controlling these units, wherein the control unit saves updated property information in the property-information saving unit each time the frame image is saved in the image saving unit.

摘要翻译： 可以检查观察成功或失败以及观察历史，而不等待观察完全完成，从而节省观察所需的时间和精力，并避免丢失观察珍贵样品等的机会。提供一种显微镜装置，包括 图像获取单元，用于在改变多个参数的同时获取多个帧图像; 图像保存单元，用于连续地保存由图像获取单元获取的帧图像; 属性信息保存单元，用于保存其中保存的帧图像的识别信息与参数相关联的属性信息; 以及用于控制这些单元的控制单元，其中，每当帧图像被保存在图像保存单元中时，控制单元将更新的属性信息保存在属性信息保存单元中。

公开(公告)号：US20070295892A1

公开(公告)日：2007-12-27

申请号：US11811792

申请日：2007-06-12

申请人： Shigeru Kanegae ,  Tatsuo Nakata

发明人： Shigeru Kanegae ,  Tatsuo Nakata

IPC分类号： H01J40/14 ,  G02F1/01

CPC分类号： G01N21/6458

摘要： The invention provides a light-stimulus illumination apparatus comprising a light source for emitting light-stimulus laser light; a scanning unit including at least one acousto-optic device for scanning the light-stimulus laser light emitted from the light source in a direction intersecting an optical axis; and a control unit for controlling the scanning unit. The control unit controls the scanning unit so that the light-stimulus laser light irradiates a plurality of spatially separated regions in a time-division manner.

摘要翻译： 本发明提供了一种光刺激照明装置，包括用于发射光激励激光的光源; 扫描单元，包括至少一个声光装置，用于沿与光轴相交的方向扫描从光源发射的光激励激光; 以及用于控制扫描单元的控制单元。 控制单元控制扫描单元，使得激光激光以时分方式照射多个空间分离的区域。

公开(公告)号：US20070269796A1

公开(公告)日：2007-11-22

申请号：US11800119

申请日：2007-05-04

申请人： Mitsuhiro Nakano ,  Tatsuo Nakata

发明人： Mitsuhiro Nakano ,  Tatsuo Nakata

IPC分类号： G06F19/00 ,  G06T7/20 ,  C12Q1/00

CPC分类号： C12M41/36 ,  G02B21/0012 ,  G02B21/16 ,  G02B21/32

摘要： The invention provides a microscope apparatus, used for identifying the cell phases of a plurality of cells mounted on a stage, including an analysis/observation optical system used for acquiring an observed-image of the cells and a stimulus optical system used for applying an optical stimulus to prescribed cells. Using these optical systems, the cell phases of the cells mounted on the stage are identified, an optical stimulus is applied to the cells, and the state of the cells before and after applying the stimulus is observed.

摘要翻译： 本发明提供了一种用于识别安装在舞台上的多个电池的电池相位的显微镜装置，包括用于获取电池的观察图像的分析/观察光学系统和用于施加光学器件的刺激光学系统 刺激规定的细胞。 使用这些光学系统，鉴定安装在载物台上的细胞的细胞相，向细胞施加光学刺激，并观察施加刺激之前和之后的细胞状态。

公开(公告)号：US20060278530A1

公开(公告)日：2006-12-14

申请号：US11447761

申请日：2006-06-06

申请人： Kunihiko Sasaki ,  Yasunari Matsukawa ,  Hiroshi Sasaki ,  Tatsuo Nakata

发明人： Kunihiko Sasaki ,  Yasunari Matsukawa ,  Hiroshi Sasaki ,  Tatsuo Nakata

IPC分类号： B01D57/02 ,  B01D59/42 ,  B01D59/50

CPC分类号： G02B21/008 ,  G01N21/6458

摘要： A scanning laser microscope that can quantitatively display a laser irradiation power on a display unit and that can suppress fluctuations in the laser irradiation power is provided. The scanning laser microscope includes a light source configured to emit laser light, a scanning unit configured to scan the laser light emitted from the light source on a sample, a photodetector configured to detect light from the sample, a processing unit configured to convert a signal from the photodetector into an image signal of the sample and to output the image signal, a reference light detector configured to detect part of the laser light emitted from the light source as a reference light signal, a laser light control unit configured to control the power of the laser light emitted from the light source, an irradiation-power calculation unit configured to calculate an irradiation power value at the sample on the basis of the reference light signal detected by the reference light detector, and a display unit configured to display the calculated irradiation power value at the sample.

摘要翻译： 提供了一种可以在显示单元上定量显示激光照射功率并且可以抑制激光照射功率的波动的扫描激光显微镜。 该扫描激光显微镜包括配置为发射激光的光源，被配置为扫描从样品上的光源发射的激光的扫描单元，被配置为检测来自样品的光的光电检测器，被配置为将信号 从光电检测器到样品的图像信号并输出​​图像信号;参考光检测器，被配置为检测从光源发射的激光的一部分作为参考光信号;激光控制单元，被配置为控制功率 照射功率计算单元，被配置为基于由参考光检测器检测的参考光信号计算样本处的照射功率值;以及显示单元，被配置为显示所计算的 样品照射功率值。

公开(公告)号：US20060158721A1

公开(公告)日：2006-07-20

申请号：US11362473

申请日：2006-02-24

申请人： Tatsuo Nakata ,  Masahiro Aoki

发明人： Tatsuo Nakata ,  Masahiro Aoki

IPC分类号： G02B21/06

CPC分类号： G02B21/16 ,  G02B21/0068 ,  G02B21/0076

摘要： A confocal microscope comprises a light source emitting a polarized light beam, an objective lens irradiating the polarized light beam, which is deflected and scanned by the optical scanner, to the sample as an excitation light beam, a wavelength separator detecting a necessary wavelength band from a polarized fluorescence emitted from the sample which is excited by the polarized light beam, and a photodetector unit having a polarization property extractor extracting a fluorescence with a predetermined polarization property from the fluorescence detected with the wavelength separator, a wavelength selector selecting a wavelength of the fluorescence extracted by the polarization property extractor, and a photodetector detecting the fluorescence selected by the wavelength selector.

公开(公告)号：US20060146402A1

公开(公告)日：2006-07-06

申请号：US11318406

申请日：2005-12-22

申请人： Tatsuo Nakata

发明人： Tatsuo Nakata

IPC分类号： G02B21/00 ,  G02B21/06

CPC分类号： G02B21/0024 ,  G02B21/0032

摘要： A laser scanning microscope includes a light source unit that emits laser light, a scanning unit that scans the laser light, an optical system that converges the laser light into a specimen, a light detecting unit that detects fluorescence generated from the specimen, and a filter unit located on an optical path of light entering the light detecting unit. The filter unit includes a short pass filter that has a large number of independent filter cells in different wavelength ranges formed in line on a common substrate, and a long pass filter that has a large number of independent filter cells in different wavelength ranges formed in line on a common substrate. The filter unit has a characteristic of a bandpass filter having a desired wavelength range owing to a combination of the filter cells of the short pass filter and the filter cells of the long pass filter.

摘要翻译： 激光扫描显微镜包括发射激光的光源单元，扫描激光的扫描单元，将激光会聚到样本的光学系统，检测从样本产生的荧光的光检测单元和滤波器 单元位于进入光检测单元的光的光路上。 滤波器单元包括短通滤波器，该短通滤波器具有大量在公共基板上成行的不同波长范围内的独立滤波单元，以及具有大量不同波长范围内的独立滤波单元的长通滤波器， 在共同的基底上。 由于短路滤波器的滤波器单元和长距离滤波器的滤波器单元的组合，滤波单元具有期望波长范围的带通滤波器的特性。

公开(公告)号：US20060126170A1

公开(公告)日：2006-06-15

申请号：US11299210

申请日：2005-12-08

申请人： Yusuke Yamashita ,  Tatsuo Nakata ,  Shingo Suzuki

发明人： Yusuke Yamashita ,  Tatsuo Nakata ,  Shingo Suzuki

IPC分类号： G02B21/34

CPC分类号： G01N21/6458 ,  G02B21/16

摘要： Light emitted from a light source is condensed and a sample is irradiated by the condensed beam by an optical system. The light emitted from the sample because of the irradiation is incident on a light detection unit via a wavelength selective optical device having different transmittances depending on wavelengths, a dispersive unit for spectrally resolving light, a wavelength range selection unit for selecting light having a wavelength in a prescribed wavelength range among the light resolved spectrally. A correction unit adjusts a light intensity signal output from the light detection unit based on at least one of a wavelength characteristics of the wavelength selective optical device and the light detection unit respectively.

摘要翻译： 从光源发射的光被冷凝，并且样品被光学系统的聚光束照射。 由于照射而从样品发射的光通过取决于波长的透射率不同的波长选择光学器件入射到光检测单元上，用于光谱分解光的分散单元，用于选择具有波长的光的波长范围选择单元 在光谱中解析光的规定波长范围。 校正单元分别基于波长选择光学器件和光检测单元的波长特性中的至少一个来调整从光检测单元输出的光强度信号。

公开(公告)号：US07015444B2

公开(公告)日：2006-03-21

申请号：US11085047

申请日：2005-03-22

申请人： Yoshihiro Kawano ,  Tadashi Hirata ,  Tatsuo Nakata ,  Yoshihisa Tanikawa

发明人： Yoshihiro Kawano ,  Tadashi Hirata ,  Tatsuo Nakata ,  Yoshihisa Tanikawa

IPC分类号： G02B27/40 ,  G02B27/64 ,  G02B7/04

CPC分类号： G02B21/0048 ,  G02B21/004 ,  G02B21/0076

摘要： The invention provides an optical-scanning examination apparatus with a simple configuration, in which the resolution of acquired images can be freely changed and in which the fluorescence image intensity and examination depth can be adjusted to suit the purpose of examination. The optical-scanning examination apparatus includes a light source unit; a focusing lens for forming a first intermediate image of excitation light; an imaging lens; a first objective lens; an optical fiber bundle; a second objective lens; and an imaging unit for imaging return light that returns via the second objective lens, the optical fiber bundle, the first objective lens, and the imaging lens. In addition, a scanning mirror device, which is disposed at the first intermediate image position, is formed of a plurality of mirrors that simultaneously receive the first intermediate image and that can be selectively turned on and off.

摘要翻译： 本发明提供了一种具有简单结构的光学扫描检查装置，其中可以自由地改变所获取的图像的分辨率，并且可以调整荧光图像强度和检查深度以适应检查的目的。 光扫描检查装置包括：光源单元; 用于形成激发光的第一中间图像的聚焦透镜; 成像透镜; 第一个物镜; 光纤束; 第二个物镜; 以及用于对通过第二物镜，光纤束，第一物镜和成像透镜返回的返回光成像的成像单元。 此外，设置在第一中间图像位置的扫描镜装置由同时接收第一中间图像并且可以选择性地打开和关闭的多个反射镜形成。

公开(公告)号：US20050280818A1

公开(公告)日：2005-12-22

申请号：US11153635

申请日：2005-06-15

申请人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

发明人： Yusuke Yamashita ,  Kei Tsuyuki ,  Tatsuo Nakata

IPC分类号： G01J3/30 ,  G01N21/64 ,  G02B21/00 ,  G02B21/36

CPC分类号： G01N21/6458 ,  G02B21/006 ,  G02B21/008 ,  G02B21/32 ,  G02B21/367

摘要： A confocal observation system, comprising, an image acquisition unit for acquiring optical cross sectional images of a three-dimensional specimen, a three-dimensional image construction unit for constructing a three-dimensional image from the optical cross sectional images acquired by the image acquisition unit, a specification unit for specifying a desired three-dimensional region in the three-dimensional image constructed by the three-dimensional image construction unit, and a region acquisition unit for acquiring a cross sectional region to be irradiated with excitation light or stimulation light based on the three-dimensional region specified by the specification unit, wherein the excitation light or the stimulation light irradiates a region in the three-dimensional specimen corresponding to the cross sectional region acquired by the region acquisition unit.

摘要翻译： 一种共聚焦观测系统，包括：用于获取三维样本的光学截面图像的图像获取单元，用于从由图像获取单元获取的光学横截面图像构建三维图像的三维图像构造单元 ，用于指定由三维图像构造单元构成的三维图像中的所需三维区域的指定单元，以及区域获取单元，用于获取基于以下的激励光或刺激光照射的横截面区域： 由所述指定单元指定的三维区域，其中所述激发光或所述刺激光照射所述三维样本中与所述区域获取单元获取的横截面区域相对应的区域。