公开(公告)号：US20070245431A1

公开(公告)日：2007-10-18

申请号：US11686850

申请日：2007-03-15

申请人： James Metz ,  Jerry Kuner ,  James Lippmeier ,  Maurice Moloney ,  Cory Nykiforuk

发明人： James Metz ,  Jerry Kuner ,  James Lippmeier ,  Maurice Moloney ,  Cory Nykiforuk

IPC分类号： A01H1/00 ,  C12N9/04 ,  C12N15/82 ,  C12N5/04

CPC分类号： C12N9/1029 ,  C12N9/93 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472 ,  Y02P20/52

摘要： Disclosed are novel acyl-CoA synthetases and novel acyltransferases, nucleic acid molecules encoding the same, recombinant nucleic acid molecules and recombinant host cells comprising such nucleic acid molecules, genetically modified organisms (microorganisms and plants) comprising the same, and methods of making and using the same. Also disclosed are genetically modified organisms (e.g., plants, microorganisms) that have been genetically modified to express a PKS-like system for the production of PUFAs (a PUFA PKS system or PUFA synthase), wherein the organisms have been modified to express an acyl-CoA synthetase, to express an acyl transferase, to delete or inactivate a fatty acid synthase (FAS) expressed by the organism, to reduce competition for malonyl CoA with the PUFA synthase or to increase the level of malonyl CoA in the plant or plant cell, and in one aspect, to inhibit KASII or KASIII. Additional modifications, and methods to make and use such organisms, in addition to PUFAs and oils obtained from such organisms, are disclosed, alone with various products including such PUFAs and oils.

摘要翻译： 公开了新型酰基辅酶A合成酶和新型酰基转移酶，编码该酰基转移酶的核酸分子，重组核酸分子和包含该核酸分子的重组宿主细胞，包含该核酸分子的转基因生物（微生物和植物），以及制备和使用 一样。 还公开了经遗传修饰以表达用于生产PUFA（PUFA PKS系统或PUFA合酶）的PKS样系统的遗传修饰生物体（例如，植物，微生物），其中生物体被修饰以表达酰基 -CoA合成酶，以表达酰基转移酶，以删除或失活由生物表达的脂肪酸合成酶（FAS），以减少与PUFA合酶的丙二酰辅酶A竞争或增加植物或植物细胞中丙二酰辅酶A的水平 ，并且在一个方面，抑制KASII或KASIII。 除了PUFA和从这些生物获得的油之外，还公开了另外的修饰和制造和使用这些生物体的方法，其中包括各种产品，包括这种PUFA和油。

公开(公告)号：US20080044867A1

公开(公告)日：2008-02-21

申请号：US11777278

申请日：2007-07-12

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： C12P7/64 ,  C12N1/20 ,  C12N15/00 ,  C12N15/11 ,  C12N5/04

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

摘要翻译： 本发明通常涉及从分离自非细菌生物体或衍生自非细菌生物体的多不饱和脂肪酸（PUFA）聚酮化合物合酶（PKS）系统，其同源物，分离的核酸分子和编码这种PUFA PKS的生物活性结构域的重组核酸分子 系统，涉及包含PUFA PKS系统的转基因生物，制备和使用此类体系用于生产感兴趣的生物活性分子的方法，以及鉴定具有这种PUFA PKS系统的新细菌和非细菌微生物的新方法。

公开(公告)号：US20080038378A1

公开(公告)日：2008-02-14

申请号：US11777220

申请日：2007-07-12

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： A61K36/00 ,  A23L1/00 ,  C07C57/00

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080026438A1

公开(公告)日：2008-01-31

申请号：US11689587

申请日：2007-03-22

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/21 ,  C12N5/04

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20070089199A1

公开(公告)日：2007-04-19

申请号：US11452138

申请日：2006-06-12

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： A01H1/00 ,  C07H21/04 ,  C12P7/64 ,  C12N5/04 ,  C12N15/82 ,  C12N15/74 ,  C12N1/18

CPC分类号： C12P7/6472 ,  C12N15/52 ,  C12P7/6427 ,  C12P7/6463

摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

摘要翻译： 公开了来自裂殖壶菌的完全多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）系统及其生物活性片段及其同源物。 更具体地，本发明涉及编码这种PUFA PKS系统的核酸，其包含这种PUFA PKS系统的蛋白质和结构域到包含这种PUFA PKS系统的转基因生物（植物和微生物），以及制备和使用 本文公开的PUFA PKS系统。 本发明还涉及基因修饰的植物和微生物以及通过操作PUFA聚酮化合物合酶（PKS）系统来有效地产生富含多种不饱和脂肪酸（PUFA）以及其它生物活性分子的脂质的方法。

公开(公告)号：US20050266440A1

公开(公告)日：2005-12-01

申请号：US11087100

申请日：2005-03-21

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07K14/28 ,  C07K14/405 ,  C12N1/21 ,  C12N5/04 ,  C12N9/10 ,  C12N15/52 ,  C12N15/74 ,  C12N15/82 ,  C12P7/62 ,  C12P7/64 ,  C12P21/06 ,  C12Q1/68

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20050191679A1

公开(公告)日：2005-09-01

申请号：US11058046

申请日：2005-02-14

申请人： James Metz ,  Craig Weaver ,  Jerry Kuner

发明人： James Metz ,  Craig Weaver ,  Jerry Kuner

IPC分类号： A61K31/202 ,  C07H21/04 ,  C12N1/10 ,  C12N9/10 ,  C12N9/80 ,  C12P7/64 ,  C12Q1/68

CPC分类号： C12N9/1029

摘要： Disclosed are a fatty acid synthase (FAS) from Schizochytrium, biologically active fragments and homologues thereof, a nucleic acid sequence encoding such FAS, fragments and homologues thereof, the gene encoding Schizochytrium FAS, host cells and organisms that recombinantly express the FAS, host cells and organisms in which the expression and/or activity of the endogenous FAS has been attenuated, and various methods for making and using any of these proteins, nucleic acid molecules, genes, host cells or organisms.

摘要翻译： 公开了来自裂殖壶菌的脂肪酸合成酶（FAS），其生物活性片段及其同源物，编码这种FAS的核酸序列，其片段和同系物，编码裂殖酵母FAS的基因，重组表达FAS的宿主细胞和生物体宿主细胞 和其中内源性FAS的表达和/或活性已被减弱的生物体，以及用于制备和使用这些蛋白质，核酸分子，基因，宿主细胞或生物体中的任何一种的各种方法。

公开(公告)号：US20080038791A1

公开(公告)日：2008-02-14

申请号：US11777275

申请日：2007-07-12

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： C12P7/64 ,  C12N1/00 ,  C12N1/12 ,  C12N1/21

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080032351A1

公开(公告)日：2008-02-07

申请号：US11689596

申请日：2007-03-22

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： C12N1/20 ,  C07H21/04 ,  C12N5/04 ,  C12N5/10

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080005811A1

公开(公告)日：2008-01-03

申请号：US11668333

申请日：2007-01-29

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： A01H1/00 ,  A23L1/36 ,  C07H21/04 ,  C11B1/00 ,  C12N1/19 ,  C12N1/21 ,  C12N15/74 ,  C12N15/82 ,  C12N5/10 ,  C12P7/64

CPC分类号： C12P7/6472 ,  A23D9/00 ,  C12N9/001 ,  C12N9/1029 ,  C12N9/88 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6463

摘要： Disclosed are the complete polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems from Schizochytrium, and biologically active fragments and homologues thereof. More particularly, this invention relates to nucleic acids encoding such PUFA PKS systems, to proteins and domains thereof that comprise such PUFA PKS systems, to genetically modified organisms (plants and microorganisms) comprising such PUFA PKS systems, and to methods of making and using the PUFA PKS systems disclosed herein. This invention also relates to genetically modified plants and microorganisms and methods to efficiently produce lipids enriched in various polyunsaturated fatty acids (PUFAs) as well as other bioactive molecules by manipulation of a PUFA polyketide synthase (PKS) system.

摘要翻译： 公开了来自裂殖壶菌的完全多不饱和脂肪酸（PUFA）聚酮化合物合成酶（PKS）系统及其生物活性片段及其同源物。 更具体地，本发明涉及编码这种PUFA PKS系统的核酸，其包含这种PUFA PKS系统的蛋白质和结构域到包含这种PUFA PKS系统的转基因生物（植物和微生物），以及制备和使用 本文公开的PUFA PKS系统。 本发明还涉及基因修饰的植物和微生物以及通过操作PUFA聚酮化合物合酶（PKS）系统来有效地产生富含多种不饱和脂肪酸（PUFA）以及其它生物活性分子的脂质的方法。