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    Recombinant Bacterial Host Cell for Protein Expression
    12.
    发明申请
    Recombinant Bacterial Host Cell for Protein Expression 有权
    用于蛋白表达的重组细菌宿主细胞

    公开(公告)号：US20150111249A1

    公开(公告)日：2015-04-23

    申请号：US14400068

    申请日：2013-05-13

    Applicant: UCB PHARMA S.A.

    Inventor： Philip Jonathan Bassett ,  David Paul Humphreys ,  Pareshkumar Manjibhai Patel

    IPC: C12P21/00

    CPC classification number: C12P21/00 ,  C07K16/283 ,  C07K2317/10 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02

    Abstract: The present disclosure relates to a recombinant gram-negative bacterial cell comprising: a.) a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and b.) a gene capable of expressing or overexpressing one or more proteins capable of facilitating protein folding, such as FkpA, Skp, SurA, PPiA and PPiD, wherein the cell has reduced Tsp protein activity compared to a wild-type cell, methods employing the cells, use of the cells in the expression of proteins in particular antibodies, such as anti Fc Rn antibodies and proteins made by the methods described herein.

    Abstract translation: 本公开涉及重组革兰氏阴性细菌细胞，其包含：a）编码具有在一个或多个选自D133，H145，H157，N31，R62，I70，Q73， C94，S95，V98，Q99，R100，L108，Y115，V135，L136，G140，R144和G147，以及b）能够表达或过表达能够促进蛋白质折叠的一种或多种蛋白质的基因，例如FkpA，Skp， SurA，PPiA和PPiD，其中所述细胞与野生型细胞相比具有降低的Tsp蛋白活性，使用所述细胞的方法，所述细胞在蛋白质表达特别抗体中的用途，例如抗Fc Rn抗体和由 本文描述的方法。

    Multivalent antibodies
    14.
    发明授权
    Multivalent antibodies 有权

    公开(公告)号：US10421816B2

    公开(公告)日：2019-09-24

    申请号：US15165131

    申请日：2016-05-26

    Applicant: UCB PHARMA S.A.

    Inventor： Ralph Adams ,  Emma Dave ,  David Paul Humphreys

    IPC: C07K16/46 ,  C07K16/28 ,  C07K7/06 ,  C07K7/08 ,  C07K16/18

    Abstract: The present invention provides a multivalent antibody or a heavy/light chain component thereof comprising: a heavy chain comprising a constant region fragment, said constant region fragment located between two variable domains which are not a cognate pair, the heavy chain further comprising an Fc region with at least one domain selected from CH2, CH3 and combinations thereof, with the proviso that the heavy chain contains no more than one CH1 domain and only contains two variable domains, and a light chain comprising a constant region fragment located between two variable domains which are not a cognate pair, wherein said heavy and light chains are aligned to provide a first binding site formed by a first cognate pair of variable domains and a second binding site formed by a second cognate pair of variable domains.

    Antibodies of the Class IGG4
    19.
    发明申请
    Antibodies of the Class IGG4 审中-公开

    公开(公告)号：US20200157204A1

    公开(公告)日：2020-05-21

    申请号：US16717671

    申请日：2019-12-17

    Applicant: UCB PHARMA S.A.

    Inventor： David Paul Humphreys ,  Ralph Adams ,  James Heads ,  Shirley Jane Peters

    IPC: C07K16/24 ,  A61K39/395 ,  C07K16/00

    Abstract: The present invention provides an antibody of the class IgG4 comprising at least one heavy chain which comprises a CH1 domain and a hinge region, wherein in each heavy chain: a. the inter-chain cysteine at position 127, numbered according to the Kabat numbering system, in the CH1 domain is substituted with another amino acid; and b. one or more of the amino acids positioned in the upper hinge region is substituted with cysteine.

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