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    NON-VIRAL EPISOMAL SUICIDE CONSTRUCT
    11.
    发明申请
    NON-VIRAL EPISOMAL SUICIDE CONSTRUCT 有权
    非病毒性异体结构

    公开(公告)号：US20150079682A1

    公开(公告)日：2015-03-19

    申请号：US14382816

    申请日：2013-02-27

    Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

    Inventor： Baisong Lu ,  Qingguo Zhao ,  James Yoo ,  Anthony Atala

    IPC: C12N15/85

    CPC classification number: C12N15/85 ,  C12N2800/40 ,  C12N2840/007

    Abstract: The invention includes compositions and methods for the selective expression of a target gene in a subset of cells. In certain embodiments, the present invention includes a construct comprising a first nucleic acid sequence comprising an episomal maintenance element and a second nucleic acid sequence comprising a target gene wherein the expression of the episomal maintenance element is regulated by a constitutive promoter and the expression of the target gene is regulated by a non-constitutive promoter. The construct is able to maintain episomal state, no matter whether the target gene is expressed in the cell.

    Abstract translation: 本发明包括用于在细胞子集中选择性表达靶基因的组合物和方法。 在某些实施方案中，本发明包括构建体，其包含第一核酸序列，其包含附加型维持元件和包含靶基因的第二核酸序列，其中所述附加型维持元件的表达由组成型启动子调节， 靶基因由非组成型启动子调控。 无论目标基因是否在细胞中表达，该构建体能够保持附加型状态。

    INK-JET PRINTING OF TISSUES
    17.
    发明申请
    INK-JET PRINTING OF TISSUES 审中-公开

    公开(公告)号：US20190153382A1

    公开(公告)日：2019-05-23

    申请号：US16227690

    申请日：2018-12-20

    Applicant: WAKE FOREST UNIVERSITY HEALTH SCIENCES

    Inventor： James Yoo ,  Tao Xu ,  Anthony Atala

    IPC: C12N5/00 ,  B33Y10/00 ,  B29C70/76

    Abstract: A method of forming an array of viable cells is carried out by ink-jet printing a cellular composition containing said cells on a substrate. At least two different types of viable mammalian cells are printed on the substrate, the at least two different types of viable mammalian cells selected to together form a tissue. In some embodiments at least three or four different viable mammalian cells are printed on the substrate, the cells selected to together form a tissue. In some embodiments one of the viable mammalian cell types is a stem cell. In some embodiments the method further comprises printing at least one support compound on the substrate, the support compound selected to form a tissue together with said cells. In some embodiments the method further comprises printing at least one growth factor on the substrate, the growth factor selected to cause the cells to form a tissue.

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