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    Multiply-substituted protease variants with altered net charge for use in detergents
    11.
    发明申请
    Multiply-substituted protease variants with altered net charge for use in detergents 审中-公开

    公开(公告)号：US20080274938A1

    公开(公告)日：2008-11-06

    申请号：US11588435

    申请日：2006-10-27

    申请人： Ayrookaran J. Poulose ,  Volker Schellenberger ,  James T. Kellis ,  Paech Christian ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    发明人： Ayrookaran J. Poulose ,  Volker Schellenberger ,  James T. Kellis ,  Paech Christian ,  Joanne Nadherny ,  Donald P. Naki ,  Katherine D. Collier ,  Robert M. Caldwell

    IPC分类号： C11D7/42 ,  C12N9/56

    CPC分类号： A23G4/123 ,  A61K8/66 ,  A61K2800/86 ,  A61Q11/00 ,  C11D3/386 ,  C12N9/54 ,  C12Y304/21062

    摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a low detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a high detergent concentration system than a precursor protease.Protease variants are provided that contain substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more negative or less positive compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease. Also provided are protease variants containing substitutions of the amino acids at one or more residue positions so that the substitution alters the charge at that position to make the charge more positive or less negative compared to a precursor protease and thus the protease variant is more effective in a medium detergent concentration system than a precursor protease.Further provided is a method of producing a protease variant that is more effective in a low detergent concentration system, medium detergent concentration system and high detergent concentration system than a precursor protease.

    Multi-through hole testing plate for high throughput screening
    15.
    发明授权
    Multi-through hole testing plate for high throughput screening 有权

    公开(公告)号：US06436632B1

    公开(公告)日：2002-08-20

    申请号：US09970578

    申请日：2001-10-04

    申请人： Volker Schellenberger ,  Amy Deming Liu

    发明人： Volker Schellenberger ,  Amy Deming Liu

    IPC分类号： C12Q100

    摘要： A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study. The location of the identified solution is marked based upon its location within a particular set and group of holes.