公开(公告)号：US09856466B2

公开(公告)日：2018-01-02

申请号：US14115801

申请日：2012-05-04

申请人： Neelam S. Amin ,  Katherine Augustyn ,  Joshua R. Basler ,  Luis G. Cascao-Pereira ,  Katherine D. Collier ,  Edward M. Concar ,  David A. Estell ,  James T. Kellis, Jr. ,  Euan John Magennis ,  Alexander Pisarchik ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

发明人： Neelam S. Amin ,  Katherine Augustyn ,  Joshua R. Basler ,  Luis G. Cascao-Pereira ,  Katherine D. Collier ,  Edward M. Concar ,  David A. Estell ,  James T. Kellis, Jr. ,  Euan John Magennis ,  Alexander Pisarchik ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

IPC分类号： C12N9/54 ,  C11D3/386

CPC分类号： C12N9/54 ,  C11D3/386

摘要： The present invention provides serine protease variants, more specifically subtilisin variants produced there from. Specifically, the present invention provides serine protease variants, more specifically subtilisin variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants, more specifically subtilisin variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants, more specifically subtilisin variants.

公开(公告)号：US20130260438A1

公开(公告)日：2013-10-03

申请号：US13696512

申请日：2011-05-05

申请人： Viktor Yuryevich Alekseyev ,  Neelam A Amin ,  Katherine Auguslyn ,  Luis Gustavo Cascao-Pereira ,  Katherine D. Collier ,  David A. Estell ,  James T. Kellis, JR. ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

发明人： Viktor Yuryevich Alekseyev ,  Neelam A Amin ,  Katherine Auguslyn ,  Luis Gustavo Cascao-Pereira ,  Katherine D. Collier ,  David A. Estell ,  James T. Kellis, JR. ,  Ayrookaran J. Poulose ,  Philip Frank Souter ,  Glenn Steven Ward ,  Jian Yao

IPC分类号： C12N9/54

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38681

摘要： The present invention provides serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.

摘要翻译： 本发明提供了丝氨酸蛋白酶变体。 具体地，本发明提供了与参考丝氨酸蛋白酶相比具有一个或多个取代的丝氨酸蛋白酶变体。 此外，本发明提供包含这些丝氨酸蛋白酶变体的组合物。 在一些实施方案中，本发明提供了包含这些丝氨酸蛋白酶变体中的至少一种的清洁组合物。

公开(公告)号：US20130005029A1

公开(公告)日：2013-01-03

申请号：US13540064

申请日：2012-07-02

申请人： Luis G. CASCAO-PEREIRA ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, JR. ,  Brain E. Jones ,  Melodie Estabrook ,  Marc Kolkman ,  Chris Leeflang ,  Casper Vroemen ,  Walter Weyler

发明人： Luis G. CASCAO-PEREIRA ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, JR. ,  Brain E. Jones ,  Melodie Estabrook ,  Marc Kolkman ,  Chris Leeflang ,  Casper Vroemen ,  Walter Weyler

IPC分类号： C12N15/56 ,  C12N1/19 ,  C12N1/21 ,  C12N1/15 ,  C12N15/63 ,  C12N5/10

CPC分类号： C12N9/2417 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L29/06

摘要： Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

摘要翻译： 描述了具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质的亲本α-淀粉酶的变体（突变体）及其使用方法。

公开(公告)号：US08236545B2

公开(公告)日：2012-08-07

申请号：US12365646

申请日：2009-02-04

申请人： Luis G. Cascao-Pereira ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, Jr. ,  Brian E Jones ,  Melodie Estabrook ,  Marc Kolkman ,  Chris Leeflang ,  Casper Vroemen ,  Walter Weyler

发明人： Luis G. Cascao-Pereira ,  Claudine Chang ,  Clement Choy ,  James T. Kellis, Jr. ,  Brian E Jones ,  Melodie Estabrook ,  Marc Kolkman ,  Chris Leeflang ,  Casper Vroemen ,  Walter Weyler

IPC分类号： C12P21/06 ,  C12N9/00 ,  C12N9/28 ,  C11D3/386 ,  C07K1/00 ,  C07H21/04

CPC分类号： C12N9/2417 ,  A21D8/042 ,  A23K20/189 ,  A23K50/75 ,  A23L29/06

摘要： Described are variants (mutants) of a parent alpha-amylase having alpha-amylase activity and exhibiting altered properties relative to the parent alpha-amylase, and methods of use, thereof.

摘要翻译： 描述了具有α-淀粉酶活性并且相对于母体α-淀粉酶具有改变的性质的亲本α-淀粉酶的变体（突变体）及其使用方法。

公开(公告)号：US08206966B2

公开(公告)日：2012-06-26

申请号：US12263886

申请日：2008-11-03

申请人： Luis G. Cascāo-Pereira ,  James T. Kellis, Jr. ,  Bradley A. Paulson ,  Scott D. Power ,  Sandra W. Ramer ,  Vivek Sharma ,  Andrew Shaw ,  Jayarama K. Shetty ,  Donald E. Ward

发明人： Luis G. Cascāo-Pereira ,  James T. Kellis, Jr. ,  Bradley A. Paulson ,  Scott D. Power ,  Sandra W. Ramer ,  Vivek Sharma ,  Andrew Shaw ,  Jayarama K. Shetty ,  Donald E. Ward

IPC分类号： C12N9/26 ,  C12N9/28 ,  C12N9/14 ,  C12N9/16 ,  C12N1/20 ,  C12N15/00 ,  C12P19/14 ,  C12P21/06 ,  C12P19/34 ,  C07H21/04 ,  C12C11/00 ,  A21D2/16 ,  C07K1/00

CPC分类号： C11D3/38681 ,  C11D3/386 ,  C12N9/2417 ,  D06M16/003

摘要： Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.

摘要翻译： 公开了包含α-淀粉酶变体的组合物，其具有α-淀粉酶活性，并且相对于其来源的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。

公开(公告)号：US20100297727A1

公开(公告)日：2010-11-25

申请号：US12602985

申请日：2008-06-06

申请人： Wolfgang Aehle ,  Luis Gustavo Cascao-Pereira ,  James T. Kellis, JR. ,  Andrew Shaw

发明人： Wolfgang Aehle ,  Luis Gustavo Cascao-Pereira ,  James T. Kellis, JR. ,  Andrew Shaw

IPC分类号： C12N9/08 ,  C12N9/48 ,  C12N9/42 ,  C12N9/26 ,  C12N9/16 ,  C12N9/18 ,  C12N9/10 ,  C12N9/02

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38681 ,  C12N9/2411 ,  C12N9/2417 ,  C12N15/1034 ,  C12Y302/01001 ,  C12Y304/21062

摘要： The present invention provides methods for engineering proteins to optimize their performance under certain environmental conditions of interest. In some embodiments, the present invention provides methods for engineering enzymes to optimize their catalytic activity under particular environmental conditions. In some preferred embodiments, the present invention provides methods for altering the net surface charge and/or surface charge distribution of enzymes {e.g., metalloproteases or serine proteases) to obtain enzyme variants that demonstrate improved performance in detergent formulations as compared to the starting or parent enzyme.

摘要翻译： 本发明提供了在某些感兴趣的环境条件下工程化蛋白质以优化其性能的方法。 在一些实施方案中，本发明提供了工程化酶以在特定环境条件下优化其催化活性的方法。 在一些优选的实施方案中，本发明提供用于改变酶（例如金属蛋白酶或丝氨酸蛋白酶）的净表面电荷和/或表面电荷分布的方法，以获得与起始或母体相比在洗涤剂配方中表现出改善的性能的酶变体 酶。

公开(公告)号：US20100192985A1

公开(公告)日：2010-08-05

申请号：US12616097

申请日：2009-11-10

申请人： Wolfgang Aehle ,  Luis G. Cascao-Pereira ,  David A. Estell ,  Frits Goedegebuur ,  James T. Kellis, JR. ,  Ayrookaran J. Poulose ,  Brian F. Schmidt

发明人： Wolfgang Aehle ,  Luis G. Cascao-Pereira ,  David A. Estell ,  Frits Goedegebuur ,  James T. Kellis, JR. ,  Ayrookaran J. Poulose ,  Brian F. Schmidt

IPC分类号： C12N9/56 ,  C07H21/00 ,  C12N15/74 ,  C12N1/21 ,  C11D3/386 ,  B08B3/00

CPC分类号： C11D3/386 ,  C11D3/38609 ,  C11D3/38618 ,  C11D3/38636 ,  C11D3/38645 ,  C11D3/38654 ,  C12N9/50 ,  C12N9/54 ,  C12Y304/21062

摘要： The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants.

摘要翻译： 本发明提供蛋白质工程方法和丝氨酸蛋白酶变体。 具体地，本发明提供了与参考丝氨酸蛋白酶相比具有一个或多个取代的丝氨酸蛋白酶变体。 此外，本发明提供包含这些丝氨酸蛋白酶变体的组合物。 在一些实施方案中，本发明提供了包含这些丝氨酸蛋白酶变体中的至少一种的清洁组合物。

公开(公告)号：US20090011489A1

公开(公告)日：2009-01-08

申请号：US11929929

申请日：2007-10-30

申请人： Ayrookaran J. Poulose ,  David A. Estell ,  James T. Kellis, JR. ,  Richard R. Bott

发明人： Ayrookaran J. Poulose ,  David A. Estell ,  James T. Kellis, JR. ,  Richard R. Bott

IPC分类号： C12N9/56 ,  C12N15/11 ,  C12N15/00 ,  C12N1/20

CPC分类号： C12N9/54 ,  C11D3/386 ,  C11D3/38609 ,  C12Y304/21062

摘要： Novel enzyme variants including protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease, Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue correspond to positions 27, 45, 170, 181, 251 and 271 of Bacillus amyloliquefaciens subtilisin. Additional variants comprising at least one additional substitution at a position selected from 1, 14, 49, 61, 87, 100, 102, 118, 128, 204 and 258 of Bacillus amyloliquefaciens subtilisin are also described.

摘要翻译： 公开了包含衍生自天然或重组非人蛋白酶的DNA序列的蛋白酶变体的新型酶变体。 变体蛋白酶一般通过体外修饰编码天然存在或重组蛋白酶的前体DNA序列获得，以产生前体蛋白酶的氨基酸序列中多个氨基酸残基的取代。这种变体蛋白酶具有 与前体蛋白酶不同的性质，例如改变的洗涤性能。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶的位置27,45,170,181,251和271。 还描述了包含在解淀粉芽孢杆菌枯草杆菌蛋白酶的1,4,14,69,61,87,100,102,118,128,204和258的位置上的至少一个另外的取代的其它变体。

公开(公告)号：US08785171B2

公开(公告)日：2014-07-22

申请号：US12963681

申请日：2010-12-09

申请人： Philip Frank Souter ,  Glenn Steven Ward ,  Viktor Yuryevich Alekseyev ,  Joshua Roy Basler ,  Luis Gustavo Cascão-Pereira ,  David A. Estell ,  Ayrookaran J. Poulose ,  James T. Kellis, Jr. ,  Alexander Pisarchik ,  Daniel Esteban Torres-Pazmino

发明人： Philip Frank Souter ,  Glenn Steven Ward ,  Viktor Yuryevich Alekseyev ,  Joshua Roy Basler ,  Luis Gustavo Cascão-Pereira ,  David A. Estell ,  Ayrookaran J. Poulose ,  James T. Kellis, Jr. ,  Alexander Pisarchik ,  Daniel Esteban Torres-Pazmino

IPC分类号： C12N9/48

CPC分类号： C11D3/38618 ,  C11D3/38609 ,  C11D3/38681 ,  C12N9/50

摘要： This invention relates to fabric and home care products comprising one or more cold water proteases and processes for making and using such products. Such compositions provide improved cleaning and freshness. Such cold water proteases may be derived from parent enzymes, including BPN′ subtilisin and subtilisin derived from Bacillus lentus, by substitution, insertion and/or deletion of one or more of the parent enzymes' amino acids.

摘要翻译： 本发明涉及包含一种或多种冷水蛋白酶的织物和家庭护理产品以及用于制造和使用这些产品的方法。 这样的组合物提供改善的清洁和新鲜度。 这样的冷水蛋白酶可以通过取代，插入和/或缺失一种或多种亲本氨基酸从母体酶衍生，包括BPN'枯草杆菌蛋白酶和源自迟缓芽孢杆菌的枯草杆菌蛋白酶。

公开(公告)号：US20130260432A1

公开(公告)日：2013-10-03

申请号：US13436612

申请日：2012-03-30

申请人： Richard R. BOTT ,  Marguerite A. Cervin ,  James T. Kellis, JR. ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Christopher Lee Rife ,  Derek H. Wells ,  Walter Weyler ,  Gregory M. Whited

发明人： Richard R. BOTT ,  Marguerite A. Cervin ,  James T. Kellis, JR. ,  Joseph C. McAuliffe ,  Andrei Miasnikov ,  Caroline M. Peres ,  Christopher Lee Rife ,  Derek H. Wells ,  Walter Weyler ,  Gregory M. Whited

IPC分类号： C12N9/88 ,  C12P5/00

CPC分类号： C12N9/88 ,  C12P5/007 ,  C12Y402/03027

摘要： The present invention provides methods and compositions comprising at least one isoprene synthase enzyme with improved catalytic activity and/or solubility. In particular, the present invention provides variant plant isoprene synthases for increased isoprene production in microbial host cells. Biosynthetically produced isoprene of the present invention finds use in the manufacture of rubber and elastomers.