摘要:
The invention pertains to a rapid and specific aptamer-based method for one-step cocaine detection with minimal reagent requirements based on an aptamer sensor that reports the presence of cocaine via the displacement and unquenching of a bound fluorophore molecule. In certain embodiments, the invention provides novel aptamers, which have reduced background fluorescence, bind a fluorophore molecule tightly, and show an increased signal gain in the presence of cocaine.
摘要:
An electronic device (100) and method for operating the same. The electronic device (100) comprises at least two working modes and comprises a connection state acquisition unit (110) and a mode switching unit (12). The connection state acquisition unit (110) is used for acquiring the connection state between the electronic device (100) and a remote control unit, and the remote control unit is used for remotely controlling the electronic device (100). The mode switching unit (12) is used for switching the working mode of the electronic device (100) according to the connected state.
摘要:
Provided are novel single-stranded oligonucleotide probes that have a triple-stem configuration in the absence of target binding to the target binding sequence. The probes also have a fluorophore and a quencher. In the absence of target binding to the target binding sequence, these single-stranded oligonucleotide probes are capable of forming self-complementary duplexes such that the probe is in the triple-stem configuration and the fluorophore is positioned adjacent the quencher. In the presence of target binding to the target binding sequence, formation of the self-complementary duplexes is inhibited such that the probe is configured to position the fluorophore away from the quencher such that a signal of the fluorophore is detectable. Also provided are methods of using the probes.
摘要:
The present invention relates to a process for the efficient preparation of enantiomerically enriched beta amino acid derivatives wherein the amino group is unprotected. The product chiral beta amino acid derivatives are useful in the asymmetric synthesis of biologically active molecules. The process comprises an enantioselective hydrogenation of an amine-unprotected prochiral beta-amino acrylic acid or derivative thereof in the presence of a rhodium metal precursor complexed with a chiral mono- or bisphosphine ligand.
摘要:
The present invention relates to a process for the efficient preparation of enantiomerically enriched beta amino acid derivatives wherein the amino group is unprotected. The product chiral beta amino acid derivatives are useful in the asymmetric synthesis of biologically active molecules. The process comprises an enantioselective hydrogenation of an amine-unprotected prochiral beta-amino acrylic acid or derivative thereof in the presence of a rhodium metal precursor complexed with a chiral mono- or bisphosphine ligand.
摘要:
The present invention relates to a process for the efficient preparation of enantiomerically enriched beta amino acid derivatives which are useful in the asymmetric synthesis of biologically active molecules. The process comprises an enantioselective hydrogenation of a prochiral beta amino acrylic acid derivative substrate in the presence of a transition metal precursor complexed with a chiral ferrocenyl diphosphine ligand.
摘要:
The invention discloses a traceable card holder, comprising a first clamp plate, a second clamp plate, an elastic band, top cover components, bottom cover components, a tracker and an elastic clamp; the first clamp plate is arranged oppositely to the second clamp plate, the elastic band is connected respectively with the first clamp plate and the second clamp plate, and the elastic band is used to drive the first clamp plate and the second clamp plate to move towards each other for clamping; the top cover components are detachably mounted on one side of the first clamp plate which is away from the second clamp plate, and the tracker is detachably mounted on the top cover components.
摘要:
The subject invention provides materials and methods for single-step detection of small molecules, e.g., natural and synthetic cannabinoids, in a sample. The subjection invention provides nucleic acids materials, e.g., aptamers (nucleic acid oligonucleotides) that can bind to natural and/or synthetic cannabinoids. The method for detecting a natural or synthetic cannabinoid in a sample comprises contacting the sample with an aptamer-based sensor selective for the natural or synthetic cannabinoid, and sensitively and rapidly detecting the natural or synthetic cannabinoid in the sample. The aptamer-based sensor comprises aptamers that can specifically binds to natural and/or synthetic cannabinoids with nanomolar dissociation constant.
摘要:
An electronic device (100) and method for operating the same. The electronic device (100) comprises at least two working modes and comprises a connection state acquisition unit (110) and a mode switching unit (12). The connection state acquisition unit (110) is used for acquiring the connection state between the electronic device (100) and a remote control unit, and the remote control unit is used for remotely controlling the electronic device (100). The mode switching unit (12) is used for switching the working mode of the electronic device (100) according to the connected state.
摘要:
A method for the determination of an analyte in a sample, the method comprising: (a) providing a catalytic polynucleotide; (b) contacting the catalytic polynucleotide with the sample so that the catalytic polynucleotide may bind to the analyte; (c) providing assay conditions such that the catalytic polynucleotide produces an optically detectable signal in the presence of the analyte; and (d) detecting the signal, thereby determining the presence of the analyte in the sample. One application of the method is for the detection of telomerase in a biological sample.