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55 条记录 (耗时 0.023 秒)

    Polynucleotide encoding UDP-glucuronyl transferase
    14.
    发明授权
    Polynucleotide encoding UDP-glucuronyl transferase 失效
    编码UDP-葡糖醛酸转移酶的多核苷酸

    公开(公告)号：US08628938B2

    公开(公告)日：2014-01-14

    申请号：US12678161

    申请日：2008-09-29

    申请人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    发明人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    IPC分类号： C12P21/06

    CPC分类号： C12N9/10 ,  C12N9/1051 ,  C12Y204/01017

    摘要： The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same, for example, a polynucleotide comprising nucleotides 1 to 1359 of SEQ ID NO: 4, nucleotides 1 to 1365 of SEQ ID NO: 10, nucleotides 1 to 1371 of SEQ ID NO: 12, and nucleotides 1 to 1371 of SEQ ID NO: 22; or a polynucleotide encoding a protein having the amino acid sequence of SEQ ID NOS: 5, 11, 13 or 23. The invention provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.

    摘要翻译： 本发明提供新的UDP-葡糖醛酸糖基转移酶和编码该多核苷酸的多核苷酸，例如包含SEQ ID NO：4的核苷酸1至1359，SEQ ID NO：10的核苷酸1至1365，SEQ ID NO：10的核苷酸1至1371，SEQ ID NO： SEQ ID NO：12和SEQ ID NO：22的核苷酸1至1371; 或编码具有SEQ ID NO：5,11,13或23的氨基酸序列的蛋白质的多核苷酸。本发明提供了具有广泛底物特异性的新型UDP-葡糖醛酸基转移酶。

    UDP-GLUCURONYL TRANSFERASE AND POLYNUCLEOTIDE ENCODING THE SAME
    15.
    发明申请
    UDP-GLUCURONYL TRANSFERASE AND POLYNUCLEOTIDE ENCODING THE SAME 失效
    UDP-GLUCURONYL转移和编码它们的多核苷酸

    公开(公告)号：US20100323402A1

    公开(公告)日：2010-12-23

    申请号：US12678161

    申请日：2008-09-29

    申请人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    发明人： Eiichiro Ono ,  Akio Noguchi ,  Yuko Fukui ,  Masako Mizutani

    IPC分类号： C12P19/60 ,  C07H21/04 ,  C07H21/00 ,  C12N9/10 ,  C12N15/63 ,  C12N1/21

    CPC分类号： C12N9/10 ,  C12N9/1051 ,  C12Y204/01017

    摘要： The present invention provides a novel UDP-glucuronosyltransferase and a polynucleotide encoding the same (for example, a polynucleotide comprising a polynucleotide consisting of one nucleotide sequence selected from the group consisting of the nucleotide sequence at positions 1 to 1359 in the nucleotide sequence represented by SEQ ID NO: 4, the nucleotide sequence at positions 1 to 1365 in the nucleotide sequence represented by SEQ ID NO: 10, the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 12, and the nucleotide sequence at positions 1 to 1371 in the nucleotide sequence represented by SEQ ID NO: 22; or a polynucleotide comprising a polynucleotide encoding a protein having one amino acid sequence selected from the group consisting of SEQ ID NOS: 5, 11, 13 and 23), etc. This provides a novel UDP-glucuronosyltransferase with a broad substrate specificity.

    摘要翻译： 本发明提供新的UDP-葡糖醛酸糖基转移酶及其编码的多核苷酸（例如，包含多核苷酸的多核苷酸，所述多核苷酸由选自SEQ ID NO：1所示的核苷酸序列中的1〜1359位的核苷酸序列的一个核苷酸序列组成 SEQ ID NO：10所示核苷酸序列中的1〜1365位核苷酸序列，SEQ ID NO：12所示核苷酸序列中1〜1371位核苷酸序列， 或包含编码具有选自SEQ ID NO：5,11,13和23的一个氨基酸序列的蛋白质的多核苷酸的多核苷酸等），等等。 这提供了具有广泛底物特异性的新型UDP-葡糖醛酸基转移酶。

    Glycosyltransferase gene
    17.
    发明授权
    Glycosyltransferase gene 失效
    糖基转移酶基因

    公开(公告)号：US07514597B2

    公开(公告)日：2009-04-07

    申请号：US10524842

    申请日：2003-08-20

    申请人： Noriko Nakamura ,  Yuko Fukui ,  Eiichiro Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    发明人： Noriko Nakamura ,  Yuko Fukui ,  Eiichiro Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    IPC分类号： C12N15/29 ,  C12N15/52 ,  C12N15/82 ,  A01H5/00

    CPC分类号： C12N15/8243 ,  C12N9/1048 ,  C12N15/825

    摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

    摘要翻译： 本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外，本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性， 证实克隆的基因在2'位编码糖基转移酶。

    Novel glycosyltransferase gene
    18.
    发明申请
    Novel glycosyltransferase gene 失效
    新型糖基转移酶基因

    公开(公告)号：US20060174377A1

    公开(公告)日：2006-08-03

    申请号：US10524842

    申请日：2003-08-20

    申请人： Noriko Nakamura ,  Yuko Fukui ,  Eichio Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    发明人： Noriko Nakamura ,  Yuko Fukui ,  Eichio Ono ,  Yoshikazu Tanaka ,  Hiroaki Okuhara

    IPC分类号： A01H1/00 ,  C07H21/04 ,  C12N9/10 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8243 ,  C12N9/1048 ,  C12N15/825

    摘要： The present invention provides an enzyme which catalyzes a reaction to transfer sugar to a hydroxyl group at position 2′ of chalcones and a gene thereof, and preferably an enzyme which catalyzes a reaction to transfer glucose to a hydroxyl group at position 2′ of the chalcones. Furthermore, the invention provides a plant whose flower color has been changed using the glycosyltransferase. Using probes corresponding to conservative regions of the glycosyltransferase, some tens of glycosyltransferase genes having nucleotide sequences corresponding to the conservative regions were cloned from flower petal cDNA libraries of carnation and the like. Furthermore, each of the glycosyltransferase genes was expressed in Escherichia coli, activity to transfer glucose to position 2′ of the chalcone, i.e., the glycosyltransferase activity at position 2′ of chalcone was confirmed in an extract solution of the Escherichia coli, and it was confirmed that cloned genes encoded the glycosyltransferase at position 2′.

    摘要翻译： 本发明提供了一种酶，其催化将糖转移到胡烷酮的2'位的羟基及其基因的反应，优选催化将葡萄糖转移至查尔酮2'位羟基的反应的酶 。 此外，本发明提供了使用糖基转移酶改变花色的植物。 使用与糖基转移酶保守区相对应的探针，从康乃馨花花瓣cDNA文库克隆了数十个具有与保守区相对应的核苷酸序列的糖基转移酶基因。 此外，每个糖基转移酶基因在大肠杆菌中表达，将葡萄糖转移到查耳酮的位置2'的活性，即在大肠杆菌的提取液中证实了查耳酮的2'位上的糖基转移酶活性， 证实克隆的基因在2'位编码糖基转移酶。

    NOVEL COMPOUND CONTAINED IN BLUE ROSE
    20.
    发明申请
    NOVEL COMPOUND CONTAINED IN BLUE ROSE 有权
    蓝色玫瑰中包含的新颖化合物

    公开(公告)号：US20120011771A1

    公开(公告)日：2012-01-19

    申请号：US13259749

    申请日：2010-03-25

    申请人： Yuko Fukui ,  Yoshikazu Tanaka

    发明人： Yuko Fukui ,  Yoshikazu Tanaka

    IPC分类号： A01G1/00 ,  C07H15/26 ,  A01H5/00 ,  C07D493/16

    CPC分类号： C12N15/825 ,  A01H5/02 ,  A01H6/749 ,  C07D493/16 ,  C07H13/08 ,  C12N9/0071 ,  C12N9/0073 ,  C12N9/1029

    摘要： Disclosed is a novel compound contained in blue rose. This novel compound, which is contained in blue rose, has a chemical structure represented by general formula (I): [wherein R1 is a group as set forth in claim 1; and R2 represents —OH, or R1 and R2 together form —O—]. Also disclosed are a rose plant containing the aforesaid compound, and a part of the same.

    摘要翻译： 公开了蓝玫瑰中含有的新化合物。 包含在蓝玫瑰中的这种新化合物具有由通式（I）表示的化学结构：[其中R1是如权利要求1所述的基团; 并且R 2表示-OH，或者R 1和R 2一起形成-O-]。 还公开了含有上述化合物的玫瑰植物及其一部分。