利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

50 条记录 (耗时 0.045 秒)

    Real-time nucleic acid detection processes and compositions
    15.
    发明授权
    Real-time nucleic acid detection processes and compositions 有权
    实时核酸检测方法和组合物

    公开(公告)号：US09261460B2

    公开(公告)日：2016-02-16

    申请号：US10096076

    申请日：2002-03-12

    申请人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Jack Coleman ,  Dakai Liu

    发明人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Jack Coleman ,  Dakai Liu

    IPC分类号： C12P19/34 ,  G01N21/64 ,  C12Q1/68

    CPC分类号： C12Q1/6818 ,  C12Q1/6834 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/686 ,  G01N21/64 ,  G01N21/6486 ,  C12Q2565/101 ,  C12Q2533/101 ,  C12Q2561/113

    摘要： This invention provides compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.

    摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。

    Property effecting and/or property exhibiting compositions for therapeutic and diagnostic uses
    16.
    发明授权
    Property effecting and/or property exhibiting compositions for therapeutic and diagnostic uses 失效
    性能影响和/或性能表现出用于治疗和诊断用途的组合物

    公开(公告)号：US08742090B2

    公开(公告)日：2014-06-03

    申请号：US08978632

    申请日：1997-11-25

    申请人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Dakai Liu ,  Norman E. Kelker ,  Dean L. Engelhardt

    发明人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Dakai Liu ,  Norman E. Kelker ,  Dean L. Engelhardt

    IPC分类号： C07H21/04

    CPC分类号： C12N15/1132 ,  C07K14/005 ,  C07K14/62 ,  C12N9/1247 ,  C12N15/87 ,  C12N2740/16322 ,  C12Q1/6897 ,  C12Q1/703 ,  C12Q2525/301 ,  C12Q2525/307

    摘要： The present invention provides an array of compositions useful for effecting and/or exhibiting changes in biological functioning and processing within cells and in biological systems containing such cells. In effect, these compositions combine chemical modifications and/or ligand additions with biological functions. The chemical modifications and/or ligand additions provide additional characteristics to the compositions without interfering substantially with their biological function. Such additional characteristics include nuclease resistance, targeting specific cells or specific cell receptors localizing to specific sites within cells and augmenting interactions between the compositions and target cells of interest as well as decreasing such interactions when desired. Also provided by the present invention are processes and kits.

    摘要翻译： 本发明提供了一组组合物，其用于在细胞内和含有这种细胞的生物系统中实现和/或表现出生物功能和加工中的变化。 实际上，这些组合物将化学修饰和/或配体添加与生物学功能相结合。 化学修饰和/或配体添加为组合物提供额外的特征，而不会基本上干扰它们的生物学功能。 这样的附加特征包括核酸酶抗性，靶向特定细胞或定位于细胞内特定位点的特定细胞受体，并且增加组合物和靶细胞之间的相互作用，并且当需要时减少这种相互作用。 本发明还提供了方法和试剂盒。

    Cell line for producing a non-retroviral vector
    18.
    发明申请
    Cell line for producing a non-retroviral vector 审中-公开
    用于产生非逆转录病毒载体的细胞系

    公开(公告)号：US20090263895A1

    公开(公告)日：2009-10-22

    申请号：US12075482

    申请日：2008-03-12

    申请人： Dakai Liu ,  Elazar Rabbani

    发明人： Dakai Liu ,  Elazar Rabbani

    IPC分类号： C12N5/08

    CPC分类号： C12N15/86 ,  C12N7/00 ,  C12N2710/10352 ,  C12N2740/13043 ,  C12N2740/16021 ,  C12N2740/16043 ,  C12N2750/14122 ,  C12N2750/14143 ,  C12N2750/14152

    摘要： Provided are novel vectors and viral vectors capable of expressing exogenous gene or exogenous nucleic acid sequences in a target cell of interest, such as T cells, bone marrow cells, epithelial cells, liver cells and the like. The nucleic acid components of the vectors may include one or more native promoter/enhancer regions having modified sequence segments, one or more non-native promoter/enhancer or non-native promoter's gene or gene segment, and a native viral vector terminator or processing signal or segment thereof. The viral vectors comprise a virus or viral portion having on the surfaces or envelopes adsorption components, one for a packaging cell line and the other for delivery to a target cell. Other viral vectors provided by this invention have two components on their surfaces or envelopes, one of which is native to the virus and the other being non-native and capable of adsorbing to the target cell while being incapable of adsorbing to a native cell for the viral vector. Packaging cell lines for propagating the vectors and viral vectors are also provided, as are novel processes for propagating any of the disclosed vectors or viral vectors.

    摘要翻译： 提供能够在靶细胞中表达外源基因或外源核酸序列的新载体和病毒载体，例如T细胞，骨髓细胞，上皮细胞，肝细胞等。 载体的核酸组分可以包括一个或多个具有修饰的序列区段的天然启动子/增强子区域，一个或多个非天然启动子/增强子或非天然启动子的基因或基因区段，以及天然病毒载体终止子或处理信号 或其片段。 病毒载体包含在表面或包膜上具有吸附组分的病毒或病毒部分，一个用于包装细胞系，另一个用于递送至靶细胞。 本发明提供的其它病毒载体在其表面或包膜上具有两个组分，其中一个组分是病毒天然的，另一个是非天然的，并且能够吸附到靶细胞，同时不能吸附到天然细胞中 病毒载体。 还提供用于繁殖载体和病毒载体的包装细胞系，以及用于繁殖任何所公开的载体或病毒载体的新方法。

    NOVEL PROPERTY EFFECTING AND/OR PROPERTY EXHIBITING COMPOSITIONS FOR THERAPEUTIC AND DIAGNOSTIC USES
    20.
    发明申请
    NOVEL PROPERTY EFFECTING AND/OR PROPERTY EXHIBITING COMPOSITIONS FOR THERAPEUTIC AND DIAGNOSTIC USES 有权
    用于治疗和诊断用途的新型物业效果和/或物业展示组合物

    公开(公告)号：US20080248564A1

    公开(公告)日：2008-10-09

    申请号：US11929897

    申请日：2007-10-30

    申请人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Dakai Liu ,  Norman E. Kelker ,  Dean L. Engelhardt

    发明人： Elazar Rabbani ,  Jannis G. Stavrianopoulos ,  James J. Donegan ,  Dakai Liu ,  Norman E. Kelker ,  Dean L. Engelhardt

    IPC分类号： C12N15/00

    CPC分类号： C12N15/1132 ,  C07K14/005 ,  C07K14/62 ,  C12N9/1247 ,  C12N15/87 ,  C12N2740/16322 ,  C12Q1/6897 ,  C12Q1/703 ,  C12Q2525/301 ,  C12Q2525/307

    摘要： The present invention provides an array of compositions useful for effecting and/or exhibiting changes in biological functioning and processing within cells and in biological systems containing such cells. In effect, these compositions combine chemical modifications and/or ligand additions with biological functions. The chemical modifications and/or ligand additions provide additional characteristics to the compositions without interfering substantially with their biological function. Such additional characteristics include nuclease resistance, targeting specific cells or specific cell receptors localizing to specific sites within cells and augmenting interactions between the compositions and target cells of interest as well as decreasing such interactions when desired. Also provided by the present invention are processes and kits.

    摘要翻译： 本发明提供了一组组合物，其用于在细胞内和含有这种细胞的生物系统中实现和/或表现出生物功能和加工中的变化。 实际上，这些组合物将化学修饰和/或配体添加与生物学功能相结合。 化学修饰和/或配体添加为组合物提供额外的特征，而不会基本上干扰它们的生物学功能。 这样的附加特征包括核酸酶抗性，靶向特定细胞或定位于细胞内特定位点的特定细胞受体，并且增加组合物和靶细胞之间的相互作用，并且当需要时减少这种相互作用。 本发明还提供了方法和试剂盒。