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公开(公告)号：US20110223606A1
公开(公告)日：2011-09-15
申请号：US13068727
申请日：2011-05-17
Applicant: Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
Inventor： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC classification number: C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
Abstract: This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solubilize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
Abstract translation: 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下从保存的临床样品中有效溶解核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US07968327B2
公开(公告)日：2011-06-28
申请号：US11893356
申请日：2007-08-14
Applicant: Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
Inventor： Gary McMaster , Joan Davies , Yunqing Ma , Yuling Luo
CPC classification number: C12N1/06 , C12N15/1003 , C12Q1/6806 , C12Q1/6841 , Y10T436/143333 , C12Q2527/101 , C12Q2523/32 , C12Q2521/537 , C12Q2545/101
Abstract: This invention provides methods of quantitating nucleic acids from problematic samples, such as aged samples, formalin fixed samples, paraffin embedded samples, samples with aneuploid cells, and cells with fragmented nucleic acids. Methods include techniques to efficiently solublize the nucleic acids under non-denaturing conditions from preserved clinical samples without resort to organic extractions, to normalize cell counts regardless of aneuploidy, to access the fragmentation state of the nucleic acids, and to provide standard curves for degraded nucleic acid samples.
Abstract translation: 本发明提供了定量来自有问题样品的核酸的方法，例如老化样品，福尔马林固定样品，石蜡包埋样品，具有非整倍体细胞的样品和具有片段化核酸的细胞。 方法包括在非变性条件下有效地从保存的临床样品中纯化核酸而不用有机提取的技术，使细胞计数正常化而不管非整倍体，以获得核酸的断裂状态，并为降解的核酸提供标准曲线 酸样。
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公开(公告)号：US20110059866A1
公开(公告)日：2011-03-10
申请号：US12660516
申请日：2010-02-26
Applicant: Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
Inventor： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC: C40B40/06
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US08628918B2
公开(公告)日：2014-01-14
申请号：US11431092
申请日：2006-05-08
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6837 , C12Q1/6834 , C12Q2565/543 , C12Q2537/143 , C12Q2537/125 , C12Q2565/515 , C12Q2527/107 , C12Q2563/149 , C12Q2563/143
Abstract: Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on different subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了从单个样品同时捕获两种或更多种核酸的方法。 通过在不同的颗粒子集上或在空间上可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US08426578B2
公开(公告)日：2013-04-23
申请号：US12804904
申请日：2010-07-29
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6834 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313 , C12Q2565/543 , C12Q2565/501 , C12Q2563/149 , C12Q2563/103
Abstract: Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
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Patent Agency Ranking
公开(公告)号：US20130023433A1
公开(公告)日：2013-01-24
申请号：US13498249
申请日：2010-09-28
Applicant: Yuling Luo , John James Flanagan , Nan Su , Huei-Yu Fay Wang
Inventor： Yuling Luo , John James Flanagan , Nan Su , Huei-Yu Fay Wang
CPC classification number: C12Q1/6841 , C12Q2565/543 , C12Q2525/313
Abstract: The invention relates to methods of detecting nucleic acids, including methods of detecting one or more target nucleic acid sequences in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support or suspending cells are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. The invention further relates to methods to improve probe hybridization specificity and their application in genotyping. The invention also relates to in situ detection of mis-joined nucleic acid sequences. The invention relates to reducing false positive signals and improve signal-to-background ratio in hybridization-based nucleic acid detection assay. The invention further relates to method to improve specificity in hybridization based nucleic acid using co-location probes. Compositions, tissue slides, sample of suspended cells, kits, and systems related to the methods are also described.
Abstract translation: 本发明涉及检测核酸的方法，包括在多重支链DNA测定中检测一种或多种靶核酸序列的方法。 检测到在固体支持物或悬浮细胞上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 本发明还涉及改进探针杂交特异性及其在基因分型中的应用的方法。 本发明还涉及错误连接的核酸序列的原位检测。 本发明涉及在基于杂交的核酸检测测定中减少假阳性信号并提高信号与背景比。 本发明还涉及使用共位点探针改进基于杂交的核酸的特异性的方法。 还描述了组合物，组织载玻片，悬浮细胞样品，试剂盒和与该方法相关的系统。
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公开(公告)号：US07927798B2
公开(公告)日：2011-04-19
申请号：US11543752
申请日：2006-10-04
Applicant: Zhi Zheng , Yuling Luo , Gary McMaster
Inventor： Zhi Zheng , Yuling Luo , Gary McMaster
CPC classification number: C12Q1/6816 , C12Q1/6837 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313
Abstract: Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供从全血或血浆中检测一种或多种核酸的方法。 将核酸捕获在固体支持物上并检测。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20110059442A1
公开(公告)日：2011-03-10
申请号：US12660524
申请日：2010-02-26
Applicant: Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
Inventor： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
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公开(公告)号：US20070161015A1
公开(公告)日：2007-07-12
申请号：US11543752
申请日：2006-10-04
Applicant: Zhi Zheng , Yuling Luo , Gary McMaster
Inventor： Zhi Zheng , Yuling Luo , Gary McMaster
CPC classification number: C12Q1/6816 , C12Q1/6837 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313
Abstract: Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供从全血或血浆中检测一种或多种核酸的方法。 将核酸捕获在固体支持物上并检测。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20070015188A1
公开(公告)日：2007-01-18
申请号：US11471025
申请日：2006-06-19
Applicant: Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
Inventor： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
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