Laser scanning microscope and microscopic observing method
    11.
    发明授权
    Laser scanning microscope and microscopic observing method 有权
    激光扫描显微镜和显微镜观察法

    公开(公告)号:US07724426B2

    公开(公告)日:2010-05-25

    申请号:US11805874

    申请日:2007-05-24

    IPC分类号: G02B21/06 G02F1/33

    摘要: To provide a laser scanning microscope including a laser beam source for stimulation that emits a laser beam for stimulation for applying photostimulation to a sample, a scanner that performs scanning with the laser beam for stimulation, a control device that controls the scanner, and an objective lens that condenses the laser beam for stimulation used for scanning by the scanner to the sample. The scanner has at least one acoustooptic device arranged on an optical path of the laser beam for stimulation. The control device determines a plurality of frequencies on the basis of the position and the range of a photostimulation region, and simultaneously applies the high-frequency signals of the determined plurality of frequencies to a vibrator attached to the acoustooptic device.

    摘要翻译: 为了提供一种激光扫描显微镜,包括用于刺激的激光束源,其发射用于刺激的激光束以对样品施加光刺激,使用激光束进行刺激的扫描仪,控制扫描仪的控制装置和目标 将用于扫描器扫描的刺激的激光束聚集到样品上的透镜。 扫描仪具有至少一个设置在激光束的光路上用于刺激的声光装置。 控制装置基于光刺激区域的位置和范围来确定多个频率,并且将确定的多个频率的高频信号同时施加到附接到声光装置的振动器。

    Confocal observation system
    12.
    发明授权
    Confocal observation system 有权
    共聚焦观测系统

    公开(公告)号:US07355702B2

    公开(公告)日:2008-04-08

    申请号:US11153635

    申请日:2005-06-15

    IPC分类号: G01J3/44

    摘要: A confocal observation system, comprising, an image acquisition unit for acquiring optical cross sectional images of a three-dimensional specimen, a three-dimensional image construction unit for constructing a three-dimensional image from the optical cross sectional images acquired by the image acquisition unit, a specification unit for specifying a desired three-dimensional region in the three-dimensional image constructed by the three-dimensional image construction unit, and a region acquisition unit for acquiring a cross sectional region to be irradiated with excitation light or stimulation light based on the three-dimensional region specified by the specification unit, wherein the excitation light or the stimulation light irradiates a region in the three-dimensional specimen corresponding to the cross sectional region acquired by the region acquisition unit.

    摘要翻译: 一种共聚焦观测系统,包括:用于获取三维样本的光学截面图像的图像获取单元,用于从由图像获取单元获取的光学横截面图像构建三维图像的三维图像构造单元 ,用于指定由三维图像构造单元构成的三维图像中的所需三维区域的指定单元,以及区域获取单元,用于获取基于以下的激励光或刺激光照射的横截面区域: 由所述指定单元指定的三维区域,其中所述激发光或所述刺激光照射所述三维样本中与所述区域获取单元获取的横截面区域相对应的区域。

    Scanning optical device which sets hardware in association with a depth of a focus position based on hardware set values stored in association with depths of focus positions
    13.
    发明授权
    Scanning optical device which sets hardware in association with a depth of a focus position based on hardware set values stored in association with depths of focus positions 有权
    扫描光学装置,其基于与聚焦位置的深度相关联地存储的硬件设置值来设置与焦点位置的深度相关联的硬件

    公开(公告)号:US07709773B2

    公开(公告)日:2010-05-04

    申请号:US12060432

    申请日:2008-04-01

    IPC分类号: G02B7/04 G02B27/40 G02B27/64

    CPC分类号: G01N21/6456 G02B2207/113

    摘要: A clear fluorescence image is easily obtained irrespective of variation of scattering due to variation of the depth of a focus position in a specimen. A scanning optical device includes a laser light source for emitting laser light, a scanning unit for scanning the laser light emitted from the laser light source on the specimen, a focus depth adjusting unit for adjusting the depth of the focus position in the specimen of the laser light to be scanned, a fluorescence detector for detecting fluorescence generated from the focus position of the laser light in the specimen, a reference depth information storage unit for storing the absolute height of a predetermined reference depth of the focus position of the laser light in the specimen by the focus depth adjusting unit, and a hardware setting storage unit for storing the relative height to the absolute height of the reference depth at each focus position of the laser light and at least one set value of the laser light source, the scanning unit, and the fluorescence detector in association with each other.

    摘要翻译: 不管由于样品中的焦点位置的深度的变化而导致的散射变化,容易获得清晰的荧光图像。 扫描光学装置包括用于发射激光的激光源,用于扫描从激光光源发射的激光的扫描单元,用于调整样本中的焦点位置的深度的焦点深度调节单元, 要扫描的激光,用于检测从样本中的激光的聚焦位置产生的荧光的荧光检测器,用于存储激光的聚焦位置的预定参考深度的绝对高度的参考深度信息存储单元, 通过焦点深度调整单元的样本,以及硬件设置存储单元,用于存储与激光的每个聚焦位置处的参考深度的绝对高度相关的相对高度和激光源的至少一个设定值,扫描 单位和荧光检测器相互关联。

    Laser scanning microscope apparatus
    14.
    发明授权
    Laser scanning microscope apparatus 有权
    激光扫描显微镜装置

    公开(公告)号:US07696996B2

    公开(公告)日:2010-04-13

    申请号:US11294689

    申请日:2005-12-05

    IPC分类号: G06T11/20

    CPC分类号: G02B21/002 G02B21/365

    摘要: A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.

    摘要翻译: 提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。 控制项目和时间线分别沿垂直轴和水平轴显示。 激光扫描显微镜包括图形用户界面,被配置为沿着时间线设置控制项的控制值;以及控制单元,被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息 通过图形用户界面。

    Laser scanning microscope and microscopic observing method
    15.
    发明申请
    Laser scanning microscope and microscopic observing method 有权
    激光扫描显微镜和显微镜观察法

    公开(公告)号:US20070272885A1

    公开(公告)日:2007-11-29

    申请号:US11805874

    申请日:2007-05-24

    IPC分类号: G03B42/08

    摘要: To provide a laser scanning microscope including a laser beam source for stimulation that emits a laser beam for stimulation for applying photostimulation to a sample, a scanner that performs scanning with the laser beam for stimulation, a control device that controls the scanner, and an objective lens that condenses the laser beam for stimulation used for scanning by the scanner to the sample. The scanner has at least one acoustooptic device arranged on an optical path of the laser beam for stimulation. The control device determines a plurality of frequencies on the basis of the position and the range of a photostimulation region, and simultaneously applies the high-frequency signals of the determined plurality of frequencies to a vibrator attached to the acoustooptic device.

    摘要翻译: 为了提供一种激光扫描显微镜,包括用于刺激的激光束源,其发射用于刺激的激光束以对样品施加光刺激,使用激光束进行刺激的扫描仪,控制扫描仪的控制装置和目标 将用于扫描器扫描的刺激的激光束聚集到样品上的透镜。 扫描仪具有至少一个设置在激光束的光路上用于刺激的声光装置。 控制装置基于光刺激区域的位置和范围来确定多个频率,并且将确定的多个频率的高频信号同时施加到附接到声光装置的振动器。

    Laser scanning microscope apparatus
    16.
    发明申请
    Laser scanning microscope apparatus 有权
    激光扫描显微镜装置

    公开(公告)号:US20060129353A1

    公开(公告)日:2006-06-15

    申请号:US11294689

    申请日:2005-12-05

    IPC分类号: G21C17/00

    CPC分类号: G02B21/002 G02B21/365

    摘要: A laser scanning microscope capable of quickly and accurately setting control values of control items for a microscope apparatus is provided. The control items and a time line are displayed along a vertical axis and a horizontal axis, respectively. The laser scanning microscope includes a graphical user interface configured to set the control values of the control items along the time line and a control unit configured to acquire luminance information of a specimen by irradiating the specimen with a laser beam in accordance with the control values set by the graphical user interface.

    摘要翻译: 提供能够快速准确地设定显微镜装置的控制项的控制值的激光扫描显微镜。 控制项目和时间线分别沿垂直轴和水平轴显示。 激光扫描显微镜包括图形用户界面,被配置为沿着时间线设置控制项的控制值;以及控制单元,被配置为通过根据控制值设定用激光束照射样本来获取样本的亮度信息 通过图形用户界面。

    Observation apparatus and observation method
    19.
    发明授权
    Observation apparatus and observation method 有权
    观察仪器和观察方法

    公开(公告)号:US08913819B2

    公开(公告)日:2014-12-16

    申请号:US13308731

    申请日:2011-12-01

    申请人: Yusuke Yamashita

    发明人: Yusuke Yamashita

    IPC分类号: G06K9/20 G06K9/62 G06K9/00

    CPC分类号: G06K9/6289 G06K9/00134

    摘要: An observation apparatus compares a plurality of image information acquired in a specific time by a plurality of image acquisition methods whose image acquisition timings are different, and includes a timer for counting observation time, and a PMT and a CCD whose image information acquisition timings are different. A storage unit stores different image information, when acquired by the PMT and the CCD, by relating each type of the acquired image information to the observation information counted by the timer. A control unit sets a display standard to associate the different types of image information stored in the storage unit, according to the observation time that has been respectively related to these types of image information, and associates the different types of image information according to the time information, on the basis of the set display standard. A monitor displays the image information associated by the control unit.

    摘要翻译: 观察装置将特定时间获取的多个图像信息与图像获取定时不同的多个图像获取方法进行比较,并且包括用于计数观察时间的定时器,以及图像信息获取定时不同的PMT和CCD 。 当由PMT和CCD获取时,存储单元通过将所获取的图像信息的每种类型与由定时器计数的观察信息相关联来存储不同的图像信息。 控制单元根据与这些类型的图像信息分别相关的观察时间,设定显示标准,将存储在存储单元中的不同类型的图像信息相关联,并根据时间将不同类型的图像信息相关联 信息,在设定的显示标准的基础上。 监视器显示由控制单元关联的图像信息。