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22 条记录 (耗时 0.026 秒)

    Method for the production of recombinant virus, DNA constructs, recombinant virus and vaccine compositions
    11.
    发明授权
    Method for the production of recombinant virus, DNA constructs, recombinant virus and vaccine compositions 有权

    公开(公告)号：US10286059B2

    公开(公告)日：2019-05-14

    申请号：US15378294

    申请日：2016-12-14

    申请人： Myrna Cristina Bonaldo ,  Ricardo Galler

    发明人： Myrna Cristina Bonaldo ,  Ricardo Galler

    IPC分类号： A61K39/12 ,  C12N7/00 ,  C07K14/005 ,  A61K39/00

    摘要： The purpose of the present invention is the production of recombinant virus through the cloning and expression of sequences of nucleotides encoding whole or part of heterologous proteins, through the following method: (a) modification of the heterologous nucleotides sequences in such way that when cloned and expressed in a vector virus, the 5′ region nucleotides present in the 5′ edge of the gene NS1 of this vector virus or of other virus or equivalent functional sequences, and in its 3′ region, the correspondent genome region in the whole or part of the domains of the stem and anchor of the protein E of the vector virus or equivalent functional sequences, and not comprising the structure and the replication of the mentioned vector virus; (b) insertion of the modified heterologous sequences in (a) in the intergenic region at the structural protein E level and of the nonstructural NS1 vector virus; (c) obtaining the non pathogenic recombinant virus with immunologic properties, having the heterologous sequences integrated in the viral genome according to the insertion described in (b) and, then expressing the heterologous antigen in such way that it can induce an appropriate immune response. The present invention is also addressed to vaccine compositions to immunize against the Flavivirus and/or other pathogens.

    Helminth-derived antigens having capacity of providing protection against parasites
    16.
    发明申请
    Helminth-derived antigens having capacity of providing protection against parasites 有权
    蠕虫衍生的抗原具有提供针对寄生虫的保护的能力

    公开(公告)号：US20070021332A1

    公开(公告)日：2007-01-25

    申请号：US10544035

    申请日：2004-01-30

    申请人： Miriam Tendler ,  Naftale Katz ,  Andrew Simpson ,  Isaias Raw ,  Paulo Ho ,  Celso Ramos

    发明人： Miriam Tendler ,  Naftale Katz ,  Andrew Simpson ,  Isaias Raw ,  Paulo Ho ,  Celso Ramos

    IPC分类号： A61K38/17 ,  C07K14/435 ,  G01N33/569

    CPC分类号： C07K14/43559 ,  A61K38/00 ,  A61K39/00 ,  G01N33/56905

    摘要： The primary objective of the present invention is the development of new mutant forms of the Sm14 protein, for producing a greater production volume. The recombinant proteins here obtained were capable of providing protection against schistosome and fasciola infection. The level of protection of Sm14 recombinant proteins obtained in the present invention was similar to that reached in the parasite saline extract. The mutant proteins of the present invention have reached approximately 100% of renaturation after the heating at 80° C., different from wild forms of the Sm14 protein. Moreover, after storage for 2 months at 4° C., mutant proteins have shown smaller β-structure loss than wild forms that have shown formation with random structure, as demonstrated by the circular dichroism analysis, indicating the success of mutations.

    摘要翻译： 本发明的主要目的是开发新的突变形式的Sm14蛋白，用于产生更大的产量。 这里获得的重组蛋白能够提供针对血吸虫病和fasciola感染的保护。 在本发明中获得的Sm14重组蛋白的保护水平与寄生盐水提取物中达到的相似。 本发明的突变型蛋白质在80℃加热后与Sm14蛋白的野生型不同，达到约100％的复性。 此外，在4℃下保存2个月后，突变蛋白质显示比野生形式更小的β-结构损失，其显示出随机结构的形成，如通过圆二色性分析所证实的，表明突变的成功。