公开(公告)号：US07098030B2

公开(公告)日：2006-08-29

申请号：US10899509

申请日：2004-07-26

申请人： David B. Rozema ,  Vladimir G. Budker ,  James E. Hagstrom ,  Vladimir Trubetskoy ,  Jon A. Wolff ,  Sean D. Monahan ,  Paul M. Slattum

发明人： David B. Rozema ,  Vladimir G. Budker ,  James E. Hagstrom ,  Vladimir Trubetskoy ,  Jon A. Wolff ,  Sean D. Monahan ,  Paul M. Slattum

IPC分类号： C12N15/88

CPC分类号： C12N15/88 ,  A61K47/58 ,  A61K47/59 ,  A61K47/645

摘要： An polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.

公开(公告)号：US07094605B2

公开(公告)日：2006-08-22

申请号：US11046598

申请日：2005-01-28

申请人： Darren H. Wakefield ,  David B. Rozema ,  Jon A. Wolff ,  Vladimir Trubetskoy ,  James E. Hagstrom ,  Vladimir G. Budker ,  Jason Klein ,  So Wong

发明人： Darren H. Wakefield ,  David B. Rozema ,  Jon A. Wolff ,  Vladimir Trubetskoy ,  James E. Hagstrom ,  Vladimir G. Budker ,  Jason Klein ,  So Wong

IPC分类号： C12N15/88

CPC分类号： C12N15/87 ,  A61K47/6455 ,  C12N15/88

摘要： Polyampholyte are able to condense nucleic acid to form small complexes which can be utilized in the delivery of nucleic acid to mammalian cells. The polyampholytes can be formed prior to interaction with nucleic acid or they can be formed in the presence of nucleic acid. Stabilized polycation/nucleic acid complexes can be modified to reduce the positive charge of the polycation and add targeting ligands without destabilizing the complex. The resultant particles retain their small size and are more effective in delivery of nucleic acid to cells in vivo.

公开(公告)号：US07049144B2

公开(公告)日：2006-05-23

申请号：US10004763

申请日：2001-12-05

申请人： Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  Vladimir S. Trubetskoy ,  Paul M. Slattum ,  Lisa J. Hanson

发明人： Jon A. Wolff ,  James E. Hagstrom ,  Vladimir G. Budker ,  Vladimir S. Trubetskoy ,  Paul M. Slattum ,  Lisa J. Hanson

IPC分类号： C12N15/88

CPC分类号： C12N15/87 ,  A61K47/59 ,  A61K47/60 ,  A61K47/645 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0025 ,  A61K48/0041

摘要： A method of forming polymers in the presence of nucleic acid using template polymerization. Also, a method of having the polymerization occur in heterophase systems. These methods can be used for the delivery of nucleic acids, for condensing the nucleic acid, for forming nucleic acid binding polymers, for forming supramolecular complexes containing nucleic acid and polymer, and for forming an interpolyelectrolyte complex.

公开(公告)号：US07033607B2

公开(公告)日：2006-04-25

申请号：US10095789

申请日：2002-03-11

申请人： Vladimir S. Trubetskoy ,  James E. Hagstrom ,  Vladimir G. Budker ,  Jon A. Wolff ,  David B. Rozema ,  Sean D. Monahan

发明人： Vladimir S. Trubetskoy ,  James E. Hagstrom ,  Vladimir G. Budker ,  Jon A. Wolff ,  David B. Rozema ,  Sean D. Monahan

IPC分类号： A61K9/127 ,  A61K31/713

CPC分类号： C12N15/88 ,  A61K47/58 ,  A61K47/59 ,  A61K47/60 ,  A61K47/645 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0025 ,  A61K48/0041 ,  C12N15/87

摘要： A polyampholyte is utilized in a condensed polynucleotide complex for purposes of nucleic acid delivery to a cell. The complex can be formed with an appropriate amount of positive and/or negative charge such that the resulting complex can be delivered to the extravascular space and may be further delivered to a cell.

公开(公告)号：US07019113B2

公开(公告)日：2006-03-28

申请号：US10444662

申请日：2003-05-23

申请人： David B. Rozema ,  Darren Wakefield ,  Jon A. Wolff ,  Kirk Ekena ,  James E. Hagstrom

发明人： David B. Rozema ,  Darren Wakefield ,  Jon A. Wolff ,  Kirk Ekena ,  James E. Hagstrom

IPC分类号： C07K1/00

CPC分类号： C12N15/87 ,  A61K38/00 ,  A61K47/60 ,  A61K48/00 ,  A61K48/0008 ,  A61K48/0041 ,  C07H21/00 ,  C07H21/04 ,  C07K7/06

摘要： An process for the reversible modification of membrane interaction of a compound is described. Modification of membrane interaction can be used to facilitate delivery of molecules to cells in vitro and in vivo. The described modifiers, which are used to reversibly inactivate the membrane active compounds, can also be utilized as cross-linkers or to reverse the charge of a molecule.

公开(公告)号：US07348453B2

公开(公告)日：2008-03-25

申请号：US10929707

申请日：2004-08-30

申请人： David B. Rozema ,  Darren Wakefield

发明人： David B. Rozema ,  Darren Wakefield

IPC分类号： C07C65/21 ,  A61K47/18

CPC分类号： C12N15/87 ,  A61K31/192 ,  A61K48/00

摘要： Described are ortho carboxy phenol derived acetals and compositions containing ortho carboxy phenol derived acetals which are useful for delivering biologically active compounds to cells. The acetals can be used to reversibly link up to three different molecules and have rapid hydrolysis kinetics in conditions which are present in a cell as well as in vivo. Cleavage of the acetal enhances delivery of the biologically active compound.

摘要翻译： 描述的是邻羧基酚衍生的缩醛和含有邻羧基酚衍生的缩醛的组合物，其可用于将生物活性化合物递送到细胞中。 缩醛可以用于可逆地连接三个不同的分子，并且在存在于细胞中以及在体内的条件下具有快速的水解动力学。 缩醛的切割增强了生物活性化合物的递送。

公开(公告)号：US07262056B2

公开(公告)日：2007-08-28

申请号：US10291342

申请日：2002-11-08

申请人： Christine Wooddell ,  Hans Herweijer ,  Jon A. Wolff

发明人： Christine Wooddell ,  Hans Herweijer ,  Jon A. Wolff

IPC分类号： C12N15/00 ,  C12N15/74 ,  C12N15/861 ,  C12N15/66 ,  C12N1/20

CPC分类号： C12N9/22 ,  C12N15/907 ,  C12N2800/90 ,  C12N2840/20

摘要： We disclose compositions and processes for enhancing transposon mediated integration of a nucleic acid molecule into another target nucleic acid molecule. Integration by an integrator complex is enhanced by cationic reagents.

摘要翻译： 我们公开了用于增强转座子介导的核酸分子整合到另一靶核酸分子中的组合物和方法。 通过阳离子试剂增强了积分器复合物的整合。

公开(公告)号：US07214369B2

公开(公告)日：2007-05-08

申请号：US10429478

申请日：2003-05-05

申请人： Jon A. Wolff ,  Vladimir G. Budker ,  James E. Hagstrom ,  Julia Hegge ,  Vladimir Subbotin

发明人： Jon A. Wolff ,  Vladimir G. Budker ,  James E. Hagstrom ,  Julia Hegge ,  Vladimir Subbotin

IPC分类号： A01N63/00 ,  A61K48/00

CPC分类号： A61K48/0075 ,  A61K38/1825 ,  A61K38/1866

摘要： A process is described for the delivery of a therapeutic polynucleotide to limb muscle tissue suffering from or potentially suffering from ischemia. The polynucleotide is inserted into a mammalian limb vessel such as an artery. Delivery efficiency and distribution is enhanced by combining injection of a solution containing the polynucleotide with the use of an externally applied cuff.

摘要翻译： 描述了将治疗性多核苷酸递送至患有或可能患有缺血的肢体肌肉组织的方法。 将多核苷酸插入哺乳动物肢体血管如动脉。 通过将含有多核苷酸的溶液的注入与使用外部应用的袖带组合来提高递送效率和分布。

公开(公告)号：US20070048254A1

公开(公告)日：2007-03-01

申请号：US11467033

申请日：2006-08-24

申请人： Zane Neal ,  Hans Herweijer

发明人： Zane Neal ,  Hans Herweijer

IPC分类号： A61K48/00 ,  A61K38/19

CPC分类号： A61K39/39 ,  A61K38/177 ,  A61K38/18 ,  A61K38/193 ,  A61K2039/545 ,  A61K2039/55522 ,  A61K2300/00

摘要： A method is provided for expanding the population of mature dendritic cells in an individual subject. Proper timing of sequential delivery of Flt3-L and GM-CSF provides improved expansion of mature dendritic cell populations over methods currently employed in the art. The expansion of mature dendritic cells can be used to enhance an immune response in the subject. The mature dendritic cells can also be isolated from the subject for use in biological research.

摘要翻译： 提供了一种用于扩大个体受试者中成熟树突细胞群体的方法。 顺序递送Flt3-L和GM-CSF的适当时机提高了成熟树突状细胞群体与本领域目前使用的方法的扩增。 成熟树突状细胞的扩增可用于增强受试者的免疫应答。 成熟树突状细胞也可以从受试者中分离出来用于生物研究。

公开(公告)号：US07091041B2

公开(公告)日：2006-08-15

申请号：US10627247

申请日：2003-07-25

申请人： Sean D. Monahan ,  Jon A. Wolff ,  Paul M. Slattum ,  James E. Hagstrom ,  Vladimir G. Budker

发明人： Sean D. Monahan ,  Jon A. Wolff ,  Paul M. Slattum ,  James E. Hagstrom ,  Vladimir G. Budker

IPC分类号： C12N15/88 ,  A61K48/00

CPC分类号： A61K48/0025 ,  A61K9/1075

摘要： A complex is described that is deliverable to a cell comprising inserting a nucleic acid or other cargo into a reverse micelle. The reverse micelle has the property to compact the nucleic acid for easier delivery.