公开(公告)号：US20040169139A1

公开(公告)日：2004-09-02

申请号：US10796105

申请日：2004-03-10

Applicant: Hitachi, Ltd.

Inventor： Minoru Sakairi ,  Yoshiaki Kato ,  Mamoru Mizumoto ,  Yuichiro Hashimoto ,  Jiro Tokita ,  Masao Suga

IPC: H01J049/00

CPC classification number: G01N1/2258 ,  G01N33/0014 ,  G01N33/0047 ,  G01N33/0049 ,  H01J49/00 ,  Y10T436/19 ,  Y10T436/196666 ,  Y10T436/24

Abstract: There was previously no monitoring method and monitoring apparatus which could measure dioxins at ppt levels and dioxin precursors at ppb levels with high sensitivity. According to this invention, organic and inorganic compounds containing highly electronegative elements are selectively ionized by atmospheric pressure chemical ionization, the ions produced are detected by a mass spectrometer, and their amount is measured. As a result, interfering substances such as nitrogen, air, hydrocarbons and carbon dioxide which are the main components of flue gas are eliminated, and dioxins or organochlorine compounds such as dioxin precursors can be selectively monitored.

Abstract translation: 以前没有监测方法和监测仪器，可以以高灵敏度的ppb水平测量ppt水平的二恶英和二恶英前体。 根据本发明，通过大气压化学电离选择性地含有高电荷性元素的有机和无机化合物，通过质谱仪检测产生的离子，测量其量。 结果，除去作为烟气主要成分的氮，空气，碳氢化合物和二氧化碳等干扰物质，可以选择性地监测二恶英或有机氯化合物如二恶英前体。

公开(公告)号：US20040169138A1

公开(公告)日：2004-09-02

申请号：US10770551

申请日：2004-02-04

Inventor： Atsushi Ootake ,  Kinya Kobayashi ,  Kiyomi Yoshinari ,  Atsumu Hirabayashi ,  Izumi Waki

IPC: H01J049/00

CPC classification number: H01J49/004 ,  H01J49/0036 ,  Y10T436/24

Abstract: The measurement throughput and the precision in sample identification are improved in a tandem type mass spectrograph. Thus, in a mass spectrum analyzing system utilizing a tandem type mass spectrograph in which the selection of an ionic species to serve as the measurement target, dissociation thereof and spectral measurement are repeated in n stages, the ionic species to be measured in MSn is selected based on the mass-to-charge ratios (m/z values) obtained as a result of the spectral analysis in MSnnull1 (nnull2), and this procedure is repeated until the sequence of a required number of amino acids is determined.

Abstract translation: 在串联型质谱仪中，测量通量和样品识别精度得到提高。 因此，在使用串联型质谱仪的质谱分析系统中，将离子物质的选择用作测定对象，在n个阶段重复其解离和光谱测定，在MS （n> = 2）中的频谱分析的结果获得的质荷比（m / z值）来选择，并且重复该过程，直到a 确定所需数量的氨基酸。

公开(公告)号：US20040164239A1

公开(公告)日：2004-08-26

申请号：US10685332

申请日：2003-10-14

Applicant: Bruker Daltonik GMBH

Inventor： Jochen Franzen

IPC: H01J049/00

CPC classification number: H01J49/401 ,  H01J49/0031

Abstract: The invention relates to a time-of-flight mass spectrometer in which a fine beam of ions is injected orthogonally into a fast pulser that pulses the ions from the fine ion beam into the spectrometer's drift region for precise determination of mass. The invention consists in increasing the duty cycle of the ions through the use of a high pulser frequency, recording the data cyclically at the same frequency, and assigning slow ions that are only measured in one of the subsequent cycles to the correct initiating pulse through the form of their lines or line patterns.

Abstract translation: 本发明涉及一种飞行时间质谱仪，其中将精细的离子束正交地注入快速脉冲发生器中，该快速脉冲发生器将离子从精细离子束脉冲进入光谱仪的漂移区域以精确确定质量。 本发明包括通过使用高脉冲发生器频率来增加离子的占空比，以相同的频率周期性地记录数据，以及将仅在随后的周期之一测量的慢离子分配给正确的起始脉冲 他们的线条或线条图案的形式。

公开(公告)号：US20040155180A1

公开(公告)日：2004-08-12

申请号：US10471454

申请日：2004-04-02

Inventor： Roman Zubarev

IPC: H01J049/00

CPC classification number: H01J49/0054

Abstract: Methods and apparatus are provided to obtain efficient Electron capture dissociation (ECD) of positive ions, particularyly useful in the mass spectrometric analysis of complex samples such as of complex mixtures and large biomolecules of peptides and proteins. Due to the low efficiency of ECD as previously used, the technique has so far only been employed with Penning cell ion cyclotron resonance mass spectrometers, where the ions are confined by a combination of magnetic and electrostatic fields. To substantially increase the efficiency of electron capture, the invention makes use of a high-intensity electron source producing a high-flux low-energy electron beam of a diameter comparable to that of the confinement volume of ions. Such a beam possesses trapping properties for positive ions. The ions confined by electron beam effectively capture electrons, which leads much shorter analysis time. The invention provides the possibility to employs ECD in other trapping and non-trapping instruments beside ICR mass spectrometers.

Abstract translation: 提供了方法和装置以获得正离子的有效电子俘获解离（ECD），特别适用于复杂样品如复合物混合物和大量生物分子的肽和蛋白质的质谱分析。 由于以前使用的ECD的效率低，该技术迄今仅用于Penning细胞离子回旋共振质谱仪，其中离子被磁场和静电场的组合限制。 为了显着提高电子俘获的效率，本发明利用高强度电子源产生直径与离子的约束体积相当的直径的高通量低能电子束。 这样的光束具有正离子的俘获特性。 由电子束限制的离子有效捕获电子，这导致更短的分析时间。 本发明提供了在ICR质谱仪旁边的其他捕获和非捕获仪器中采用ECD的可能性。

公开(公告)号：US20040129876A1

公开(公告)日：2004-07-08

申请号：US10624913

申请日：2003-07-22

Applicant: Bruker Daltonik GMBH

Inventor： Jochen Franzen

IPC: H01J049/00

CPC classification number: H01J49/16

Abstract: The invention relates to the feeding of analyte ions, generated at atmospheric pressure, efficiently into the mass spectrometer. The invention provides a lengthy ion mobility drift tube with a focusing electric field inside to guide the ions from an ionization cloud generated at atmospheric pressure towards the entrance opening of the mass spectrometer, and to dry droplets which might occur in the ionization cloud by a hot drying gas flowing through the ion mobility drift tube towards the ionization cloud.

Abstract translation: 本发明涉及在大气压下产生的分析物离子进料到质谱仪中。 本发明提供了一种长时间的离子迁移率漂移管，其内部具有聚焦电场，以引导离子从在大气压下产生的电离云朝向质谱仪的入口开口，以及干燥可能在电离云中发生的液滴 干燥气体流过离子迁移率漂移管朝向电离云。

公开(公告)号：US20040124349A1

公开(公告)日：2004-07-01

申请号：US10737243

申请日：2003-12-16

Applicant: Flender Service GmbH

Inventor： Edwin Becker ,  Mark Zundel

IPC: H01J049/00

CPC classification number: G01N27/622 ,  G01N33/2888

Abstract: A method and apparatus are provided for monitoring the quality of lubricant that is in a gear mechanism or machine that contains effective materials. A sample of the lubricant or of the vapor that escapes from the lubricant is withdrawn from the gear mechanism or machine. The sample is conveyed to an ion mobility spectrometer, where materials present in the vapor phase above the lubricant are analyzed. The change of the content and of the type of the analyzed materials in the sample is utilized as the actual condition for the aging of the lubricant.

公开(公告)号：US20040119010A1

公开(公告)日：2004-06-24

申请号：US10697991

申请日：2003-10-30

Applicant: The Regents of the University of Colorado

Inventor： M. Benjamin Perryman ,  Steve M. Helmke ,  Mark W. Duncan

IPC: H01J049/00

CPC classification number: G01N33/6848 ,  G01N33/6851

Abstract: The present invention provides for methods of quantitating the amounts of proteins or peptides, including those that are closely related isoforms, using matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS). Measurement of protein concentrations in vivo has been extremely difficult and problematic, and protein concentrations have not been shown to correlate well with mRNA levels, the standard used in the past. The present invention overcomes the deficiencies of prior methodologies by taking advantage of MALDI-TOF-MS technology and applying it to proteins and peptides in a way that allows for accurate, quantitative measurement in vivo of protein or peptide concentrations.

Abstract translation: 本发明提供了使用基质辅助激光解吸/离子化飞行时间质谱（MALDI-TOF-MS）定量蛋白质或肽的量的方法，包括具有密切相关同种型的那些。 蛋白质浓度在体内的测量是非常困难和有问题的，并且蛋白质浓度尚未显示与mRNA水平（以往使用的标准）相关性良好。 本发明通过利用MALDI-TOF-MS技术克服现有方法的缺陷，并以允许蛋白质或肽浓度的体内准确，定量测量的方式将其应用于蛋白质和肽。

公开(公告)号：US20040108456A1

公开(公告)日：2004-06-10

申请号：US10414491

申请日：2003-04-16

Applicant: University of British Columbia

Inventor： Mikhail Sudakov ,  Donald J. Douglas ,  Chuan-Fan Ding

IPC: H01J049/00 ,  B01D059/44

CPC classification number: H01J49/4225 ,  H01J49/4215

Abstract: A mass spectrometer having an elongated rod set, and a method of operating same. The rod set has an entrance end, an exit end and a longitudinal axis. Ions are admitted into the entrance end of the rod set. At least some of the ions are trapped in the rod set by producing a barrier field at an exit member adjacent to the exit end of the rod set and by producing an RF field between the rods of the rod set adjacent at least the exit end of the rod set. The RF and barrier fields interact in an extraction region adjacent to the exit end of the rod set to produce a fringing field. Ions in the extraction region are energized to mass selectively eject at least some ions of a selected mass to charge ratio axially from the rod set past the barrier field. The RF field is a two-dimensional substantially quadrupole field having a quadrupole harmonic with amplitude A2, an octopole harmonic with amplitude A4, and a hexadecapole harmonic with amplitude A8. A8 is less than A4, and A4 is greater than 0.1% of A2.

Abstract translation: 具有细长棒组的质谱仪及其操作方法。 杆组具有入口端，出口端和纵向轴线。 离子进入杆套的入口端。 至少一些离子通过在与杆组的出口端相邻的出口构件处产生阻挡场而被捕获在杆组中，并且通过在棒的杆之间产生RF场，所述RF场至少邻近于 杆组。 RF和势垒场在与棒组的出口端相邻的提取区域中相互作用以产生边缘场。 在提取区域中的离子被激发以质量选择性地弹出选定质量的至少一些离子，使其与通过屏障场的棒组件轴向轴向成比例地排出。 RF场是具有振幅A2的四极谐波，振幅A4的八极谐波和振幅A8的十六极谐波的二维基本上四极场。 A8小于A4，A4大于A2的0.1％。

公开(公告)号：US20040108451A1

公开(公告)日：2004-06-10

申请号：US10617597

申请日：2003-07-11

Inventor： Stuart C. Hansen

IPC: H01J049/00

CPC classification number: H01J49/025 ,  H01J43/246

Abstract: A multi dynode device (MDD) for electron multiplication and detection and a hybrid detector using the MDD have high peak signal output currents and large dynamic range while preserving the time-dependent information of the input event and avoiding the generation of significant distortions or artifacts on the output signal. The MDD and hybrid detector overcome saturation problems observed in conventional hybrid detectors by providing a unique electron multiplier portion that avoids the path-length differences. The MDD and hybrid detector can be used in mass spectrometry, in particular, time-of-flight mass spectrometry. The MDD comprises a plurality of dynode plates arranged in a stacked configuration. Each dynode plate in the stack has a plurality of apertures for cascading secondary electrons through the stack. Each aperture comprises a mechanical bias or offset with respect to the apertures in adjacent plates. The offset is such that the electrons will impact with one or more of the dynode plates. The MDD further comprises a power source to provide a voltage bias to the dynode plates. The power source comprises a voltage supply and a voltage divider. Each dynode plate is connected to a tap on the voltage divider such that a voltage gradient is produced along the stack. The MDD can supply high peak currents. The hybrid detector comprises an input portion having a microchannel plate MCP and an output portion having the multi dynode device (MDD). The MCP and MDD are adjacent to one another. The MDD is planar, flat, and compact like that of the MCP, such that important temporal integrity of an input signal event is preserved.

Abstract translation: 用于电子倍增和检测的多重倍增器装置（MDD）和使用MDD的混合检测器具有高峰值信号输出电流和大动态范围，同时保留输入事件的时间依赖信息，并避免产生明显的失真或伪像 输出信号。 MDD和混合式检测器通过提供避免路径长度差异的独特的电子倍增器部分来克服常规混合检测器中观察到的饱和问题。 MDD和混合检测器可用于质谱，特别是飞行时间质谱。 MDD包括以堆叠配置布置的多个倍增极板。 堆叠中的每个倍增极板具有多个用于通过堆叠层叠二次电子的孔。 每个孔包括相对于相邻板中的孔的机械偏置或偏移。 偏移使得电子将与一个或多个倍增极板相撞。 MDD还包括向倍增极板提供电压偏置的电源。 电源包括电压源和分压器。 每个倍增电极板连接到分压器上的抽头，使得沿堆叠产生电压梯度。 MDD可以提供高峰值电流。 混合式检测器包括具有微通道板MCP的输入部分和具有多重倍增器装置（MDD）的输出部分。 MCP和MDD彼此相邻。 MDD是类似于MCP的平面，平坦和紧凑的，使得保持输入信号事件的重要的时间完整性。

公开(公告)号：US20040043426A1

公开(公告)日：2004-03-04

申请号：US10465368

申请日：2003-06-18

Inventor： Jim Wells ,  Dan Erlanson ,  Andrew C. Braisted

IPC: G01N033/53 ,  H01J049/00 ,  B01D059/44 ,  C07K014/47 ,  G01N033/543

CPC classification number: C40B40/04 ,  C07D207/46 ,  C07D333/38 ,  C07D333/70 ,  C07D401/04 ,  C07D405/12 ,  C40B20/08 ,  C40B30/04 ,  G01N33/6845

Abstract: The present invention provides novel methods for ligand discovery. The inventive methods rely on a process termed nulltetheringnull where potential ligands are covalently bonded or nulltetherednull to a target and subsequently identified.

Abstract translation: 本发明提供了配体发现的新方法。 本发明的方法依赖于称为“系链”的过程，其中潜在的配体共价键合或“连接”到靶并随后鉴定。