公开(公告)号：US11751512B2

公开(公告)日：2023-09-12

申请号：US17497992

申请日：2021-10-11

申请人： ZHEJIANG UNIVERSITY

发明人： Liping Chen ,  Tingjin Wang ,  Lu Yuan ,  Ke Liu ,  Aijun Zhang ,  Yang Yang ,  Xuan Zhang ,  Yuzhuo Li ,  Zhenyu Qi

IPC分类号： A01H4/00 ,  A01G2/35

CPC分类号： A01G2/35

摘要： The present application discloses a woody rootstock for efficient grafting of solanaceous vegetables and an efficient grafting and seedling culture method thereof. According to the present application, a woody rootstock clone with high consistency is provided through tissue culture, efficient grafting is completed through sleeve grafting technology, and the grafting survival rate is improved by regulating the healing environment. A new idea for efficient industrial grafting of solanaceous vegetables is provided, scions are imparted with new features through distant grafting, and the problems of low grafting efficiency and low survival rate are solved. The method has the advantages of strong operability, simplicity, high efficiency and low cost, and provides a technical support for the industrial production of grafted seedlings of solanaceous vegetables.

公开(公告)号：US11716944B1

公开(公告)日：2023-08-08

申请号：US17948948

申请日：2022-09-20

申请人： Anhui Agricultural University ,  Anhui Wanjiang Vegetable Industry Technology Research Institute Co., Ltd.

发明人： Lingyun Yuan ,  Liting Zhang ,  Chenggang Wang ,  Xingxue Huang ,  Shidong Zhu ,  Jinlong Zhang ,  Jinfeng Hou ,  Xiaoyan Tang ,  Zegen Zhang

IPC分类号： A01H4/00 ,  C12N5/04

CPC分类号： A01H4/005 ,  C12N5/04

摘要： The present invention provides a method for improving a one-time seedling rate of microspore embryoids of Brassica campestris ssp. Chinensis Makino, including the steps of: spraying 6-BA onto a flower bud of a plant; sterilizing the flower bud with alcohol and HgCl2; releasing microspores, filtering and centrifuging to obtain purified microspores; diluting the microspores with a NLN medium, subpackaging into culture dishes, and adding phytic acid; finally, subjecting to heat shock treatment and transferring to culture in the dark until embryoids appear, and then conducting shaking culture; transferring the cultured embryos in a cotyledon stage onto a MS medium for differentiation culture, wherein the culture conditions are: 4° C., 14 h of illumination by blue-red compound light/day, and 14 days of culture; and then continuing to culture under the condition of 25° C. and 14 h of illumination by blue-red compound light/day until seedlings.

公开(公告)号：US11634724B2

公开(公告)日：2023-04-25

申请号：US16838762

申请日：2020-04-02

申请人： Altria Client Services LLC

发明人： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland

IPC分类号： C12N15/82 ,  A24B15/24 ,  A24B13/00 ,  A24B15/10 ,  C12N9/04 ,  B01L3/00 ,  G01N1/38 ,  H02N11/00 ,  B01F33/45 ,  B01F33/501 ,  B01F35/75 ,  A01H4/00 ,  C07K14/415 ,  G01N35/10 ,  G01N35/00

摘要： The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US20230110463A1

公开(公告)日：2023-04-13

申请号：US18061328

申请日：2022-12-02

申请人： CELL SCIENCE HOLDING LTD

发明人： Peter Andrew WHITTON

IPC分类号： A01H4/00 ,  A61K31/352 ,  C12N5/04 ,  A01H3/02 ,  A01H6/28 ,  A61K36/185

摘要： A method of producing cannabinoids for use in medical treatments by growing cultured Cannabis sativa plant cells through tissue culture, the method comprising the steps of: selecting Cannabis sativa leaf tissue for culture; and growing a tissue culture from the selected leaf tissue in a liquid based medium whilst controlling the light exposure of the tissue culture to control the cannabinoid content of the tissue culture. Control of the light exposure can enable the phytocannabinoid content of the grown tissue culture to be tailored to the use intended for the tissue culture. For example, the THC content of the tissue culture can be controlled to be maximised or minimised depending on the intended use. Use of tissue culture is beneficial as compared to prior art methods as it allows for genetic consistency and reduces the resources necessary to produce plant cells containing phytocannabinoids.

公开(公告)号：US11622515B2

公开(公告)日：2023-04-11

申请号：US16594733

申请日：2019-10-07

申请人： Bright Green Corporation

发明人： V. Hari

IPC分类号： A01H3/04 ,  A01H6/28 ,  A01H4/00

摘要： An method of generating and selecting mutant Cannabis plants through mutagenesis of isolated Cannabis plant cells includes subjecting plant parts of one or more Cannabis plants to a pectinase treatment to obtain living cells of the one or more Cannabis plants, suspending the living cells in a mutagenic solution comprising methane sulfonate (EMS) and dimethyl sulfoxide (DMSO) to obtain mutated Cannabis cells, centrifuging the mutated Cannabis cells to obtain pelleted cells, and providing the pelleted cells on culture media.

公开(公告)号：US20230099076A1

公开(公告)日：2023-03-30

申请号：US17955430

申请日：2022-09-28

申请人： Monsanto Technology LLC

发明人： Whitney R. Adams, JR. ,  Brandon Davis ,  Lubomyr Kucher ,  Brian J. Martinell ,  Jyoti R. Rout ,  Brenda A. Lowe

IPC分类号： A01H4/00

摘要： The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.

公开(公告)号：US11589526B2

公开(公告)日：2023-02-28

申请号：US17482723

申请日：2021-09-23

申请人： Bertrand B. Hankoua ,  Ayalew Ligaba Osena

发明人： Bertrand B. Hankoua ,  Ayalew Ligaba Osena

IPC分类号： A01H5/12 ,  A01H4/00 ,  A01G20/00 ,  A01H6/46

摘要： The present invention provides a longitudinally split immature tiller separated from a rhizome (LSITR), a cluster of multiple in vitro shoots (CMIT), a cluster of stem segments containing shoot primordia (CSSSP) and an in vitro tiller of Miscanthus x giganteus (Giant miscanthus), and their uses in propagating Miscanthus x giganteus (Giant miscanthus).

公开(公告)号：US11547071B2

公开(公告)日：2023-01-10

申请号：US17295035

申请日：2020-06-04

申请人： CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY

发明人： Ze Li ,  Yang Liu ,  Hui Zhang ,  Xiaoqin Zhang ,  Xiaohui Gao ,  Fangfang Ma ,  Jiayue Zhong ,  Xiaoyan Zhang ,  Sen Wang ,  Xiaofeng Tan ,  Ao Yang ,  Fen Bao ,  Ruonan Ma

IPC分类号： A01H4/00 ,  A01G2/10 ,  A01G24/22 ,  A01G7/06 ,  A01G23/04

摘要： A method for disinfecting explants of Kadsura coccinea stems with buds and a method for directly inducing rapid proliferation of sterile buds by using the explants of Kadsura coccinea stems with buds involve processes such as selection, treatment and disinfection of explants, primary culture, subculture proliferation culture. The problem of difficulty in tissue culture and primary culture of Kadsura coccinea stems is solved, and the advantages include low contamination rate of explants, high propagation rate and robust proliferation of axillary buds. This allows obtaining sterile axillary buds of Kadsura coccinea through tissue culture, and provides support for tissue culture, rapid propagation and factory seedling of Kadsura coccinea in the future.

公开(公告)号：US11516979B2

公开(公告)日：2022-12-06

申请号：US16315016

申请日：2017-10-27

申请人： GUANGDONG PROVINCIAL BIOENGINEERING INSTITUTE (GUANGZHOU SUGARCANE INDUSTRY RESEARCH INSTITUTE)

发明人： Zhongxing Huang ,  Donglei Sun ,  Yuanxian Xu ,  Huiyi He ,  Lina Fan ,  Fangye Lao ,  Qiuping Ling ,  Yuxing An

IPC分类号： A01H1/06 ,  A01H1/00 ,  A01H4/00

摘要： The present invention relates to a method for conducting high-throughput and directed mutagenesis for sugarcane resistance to glyphosate by plasma. The method is as follows: sugarcane embryonic calli are irradiated by a plasma instrument under a sterile condition for mutagenesis, wherein the mutagenesis power is 140˜200 W, the discharging distance is 35˜45 mm, the mutagenesis time is 110˜140 s and the protective gas is nitrogen; buffering culture, moderate/high concentration of glyphosate stress screening, differentiation into seedlings, glyphosate stress screening of bottle seedlings and stress screening via spraying glyphosate on the leave surfaces of potted plants are conducted for the treated calli. The present invention has the advantages of safe operation, simplicity, practicability, high handling capacity, low contamination, and due to implementation of directed stress screening, high screening efficiency, decreased subsequent screening workload and visual identification of resistant mutant strains.

公开(公告)号：US20220304267A1

公开(公告)日：2022-09-29

申请号：US17613340

申请日：2020-05-22

申请人： Galy Co.

发明人： Luciano Luiz Bueno ,  Paula Maria Elbl ,  Leticia Silveira De Sousa Luz

IPC分类号： A01H4/00 ,  D01F13/02

摘要： Compositions, kits, and methods for preparing a plant cell composition are provided. Compositions, kits, and methods for engineering plant cells are also provided. In some cases, compositions, kits, and methods provided herein can be used to produce cotton.