公开(公告)号：US20220252561A1

公开(公告)日：2022-08-11

申请号：US17620035

申请日：2020-06-16

Applicant: Cytiva Sweden AB

Inventor： Ana Stern

IPC: G01N30/86 ,  B01D15/34

Abstract: The present invention relates to a computer implemented method (600) performed by a chromatography apparatus (400) configured to separate molecules, having varying size, from an eluent, the method comprising obtaining (610) reference data for a chromatography column of the chromatography apparatus, wherein the reference data is indicative of elution characteristics for a set of molecular sizes, obtaining (620) an elution progress measure and a corresponding quantitative measure indicative of a concentration of molecules in an eluate of the chromatograph) apparatus, estimating (630) a size measure indicative of molecular size of the molecules in the eluate, rendering (640) a representation (300) indicative of the quantitative measure and the size measure, controlling (650) a display to display the representation to a user of the chromatography apparatus.

公开(公告)号：US20220243159A1

公开(公告)日：2022-08-04

申请号：US17611025

申请日：2020-05-26

Applicant: Cytiva Sweden AB

Inventor： Prasad Yakkar Kini ,  Shashikanth Agnihotry ,  Sarath Chandra N ,  Vinodkumar Mukuntharaj ,  Sairam C ,  Prasad Bagiyanathan ,  Kandakumar Murugesan

IPC: C12M3/00 ,  C12M1/06 ,  C12M1/00 ,  C12M1/36

Abstract: Systems (100, 700) and method for probe angle adjustment in bioreactor vessels are provided. The system (100) comprises a reactor vessel (110) having a reaction chamber (113), wherein the reaction chamber (113) has at least one opening (111). A probe support bar assembly (200, 300) is mounted on the reactor vessel (110) and a sensing probe (114) is mounted on the probe support bar (112) and the sensing probe (114) passes through the opening (111) on the reaction chamber (113). Movement of the probe support bar assembly (200, 300) changes the angle of inclination of sensing probe (114) inside the reaction chamber (113).

公开(公告)号：US11360057B2

公开(公告)日：2022-06-14

申请号：US16708291

申请日：2019-12-09

Applicant: Cytiva Sweden AB

Inventor： Klaus Gebauer ,  Jan Kranse ,  Mats Lundkvist

IPC: G01N30/60 ,  B01D15/14 ,  B33Y30/00 ,  B33Y80/00

Abstract: A fluid distributor unit comprising a channel system where one or more inlet channels (2) starting on an inlet side (3) of the distributor unit branch out successively into several channels (6) ending on the other side of the distributor unit, called the outlet side (4), characterised in that said distributor unit is provided in one single body (1) by free form fabrication.

公开(公告)号：US20220111309A1

公开(公告)日：2022-04-14

申请号：US17431219

申请日：2020-02-24

Applicant: Cytiva Sweden AB

Inventor： Matthew Townsend ,  Ian Scanlon

IPC: B01D15/18 ,  B01D15/12 ,  B01D15/34 ,  B01D15/38

Abstract: The present invention relates to a method for purifying a feed comprising at least one target product in a chromatography system having a plurality of purifying units, each having an inlet and an outlet, and a valve assembly having an outlet port and an inlet port. The inlet and the outlet of each purifying unit being connected to a respective port of the valve assembly. The method comprising loading (S10) the plurality of purifying units with feed provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly: eluting (S12) the plurality of purifying units using an elution provided through the inlet port of the valve assembly by sequentially connecting each purifying unit to the inlet port of the valve assembly: and collecting (S14) the at least one target product from the outlet port of the valve.

公开(公告)号：US11254903B2

公开(公告)日：2022-02-22

申请号：US15328763

申请日：2015-07-09

Applicant: Cytiva Sweden AB

Inventor： Michelle Louise Janas ,  Aaron Dulgar Tulloch ,  Clive Glover

IPC: C12M1/00 ,  C12M3/06 ,  C12M1/34 ,  C12M1/36 ,  C12N5/0783

Abstract: The present invention relates to cell culture in bioreactors, such as flexible cellbag bioreactors. More closely the invention relates to a method and system for determining the cell density in a bioreactor culture and for controlling the perfusion rate of a suspension culture of cells in a bioreactor, comprising measuring the oxygen uptake of primary mononuclear cells in a non-static bioreactor.

公开(公告)号：US20220018757A1

公开(公告)日：2022-01-20

申请号：US17487722

申请日：2021-09-28

Applicant: Cytiva Sweden AB

Inventor： Henrik Svanberg ,  Hanno Ehring ,  Erik Nils Ostlund

IPC: G01N21/05 ,  G02B6/38 ,  G02B6/36 ,  G02B6/42

Abstract: The present invention relates to an optical flow cell for a measuring device, having an input light guide with a light exit surface, an output light guide with a light entrance surface, said input light guide and output light guide being integrated with a holder to form an optical flow cell, and wherein the holder extends along a first axis and has a through hole for receiving a flow of a sample fluid, said through hole being transversal to said first axis, and the input light guide and output light guide further are arranged in said holder so that the light exit surface and the light entrance surface extend into said through hole and are arranged to be in optical alignment with each other and at a first distance from each other. The invention also relates to a measuring device having at least one optical flow cell.

公开(公告)号：US11136598B2

公开(公告)日：2021-10-05

申请号：US15907470

申请日：2018-02-28

Applicant: Cytiva Sweden AB

Inventor： Daniel Leif Ivansson ,  Andreas Castan

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22

Abstract: The present invention relates to an improved method for cell line development which is generally applicable to production of any therapeutic protein that can be produced using mammalian Cell lines and in particular Chinese Hamster Ovary (CHO) cells. The method combines site directed integration (SDI), expression construct components improving the post-transcriptional processing of the gene of interest (GOI), novel design of the GO genome target location and the introduction of a onetime pre-CLD host cell line selection workflow to generate a production competent cell line that can then be used in multiple CLD efforts from that point on.

公开(公告)号：US20210269888A1

公开(公告)日：2021-09-02

申请号：US17253067

申请日：2019-07-01

Applicant: Cytiva Sweden AB

Inventor： Key Hyckenberg ,  Helena Skoglar ,  Gunnar Malmquist ,  Mikael Carlsten ,  Roger Lundqvist ,  Dan Andersson ,  Linda Mathiasson ,  Lars Mattsson ,  Sara Sendabo ,  Mikael Berg

IPC: C12Q3/00 ,  C12M1/36 ,  C12M1/00 ,  C12M1/34

Abstract: The present invention relates to a method for optimizing a bioprocess purification system comprising a bioreactor configured to provide a harvest comprising a target composition, and a purification process arranged downstream the bioreactor and being configured for purification of the harvest to produce a target product having a desired characteristics. The method comprising: a) detecting (32) at least one quality attribute indicative of characteristics of the target product in a downstream process, b) identifying (33) correlations between the at least one quality attribute measured in the downstream process and parameters to control a cell culture process in the bioreactor, and c) controlling (34) the cell culture process to meet the desired characteristics based on the identified correlations, whereby the target characteristics is within a pre-determined range.

公开(公告)号：US11103846B2

公开(公告)日：2021-08-31

申请号：US16763787

申请日：2018-11-15

Applicant: Cytiva Sweden AB

Inventor： Johan Rosenquist ,  Daria Donati ,  Mia Margareta Elisabet Bennemo

IPC: B01J19/00 ,  B01J4/00 ,  B01J19/24

Abstract: The present disclosure relates to a volume tailorable manufacturing system for quality assured manufacturing of biosafety level classified biopharmaceutical products and a method for tailoring a production volume capability of a manufacturing system. The volume tailorable manufacturing system comprises one or more multi-product suites and a control facility configured to control a unidirectional flow in a circulation system of the one or more multi-product suites. The circulation system is configured to interconnect the one or more multi-product suites and comprises separated supply and return systems. The supply system comprising at least one inlet, the return system comprising at least one outlet that is paired with the inlet and provided at a spatially predetermined position from the inlet, and each inlet/outlet pair comprises a seal when not connected to an adjacent multi-product suite.

公开(公告)号：US20210253993A1

公开(公告)日：2021-08-19

申请号：US17235195

申请日：2021-04-20

Applicant: Cytiva Sweden AB

Inventor： Christian Kaisermayer

IPC: C12M1/12 ,  C12M1/00 ,  C12M1/34 ,  C12M3/00

Abstract: The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be aseptically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.