公开(公告)号：US20200256837A1

公开(公告)日：2020-08-13

申请号：US16651722

申请日：2018-03-18

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Daqi GAO ,  Zejian WANG ,  Xiaoqin ZHANG ,  Jiamin SONG

IPC: G01N33/00 ,  G01N1/24 ,  G06N3/08

Abstract: Provided is a sensory quality evaluation method for tobacco and tobacco products using an electronic nose instrument. The electronic nose instrument includes a gas sensor array module, an automatic smoke sampling system, a computer control and data analysis system, and an automatic ignition device. These components are integrated in a test box to make the instrument be structure miniaturization and work automation. The large tobacco data is established, in which the relationship between the responses of gas sensor array and the brand labels and sensory quality index scores by testing a large number of standard cigarette samples. A cascade type of modular neural networks is proposed with revised activation function and new decision-making and quantification rules to simulate the smoking and evaluating process of the professional panel. The electronic nose instrument and method realize on-site detection, discrimination and quality score estimation of a large number of tobacco and tobacco products.

公开(公告)号：US20200140830A1

公开(公告)日：2020-05-07

申请号：US16620525

申请日：2018-04-13

Applicant: JIANGSU AOSAIKANG PHARMACEUTICAL CO., LTD. ,  EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Huilei YU ,  Yan ZHANG ,  Jianhe XU ,  Qian ZHAO ,  Jiang PAN ,  Feng LIU ,  Guoqiang LIN

IPC: C12N9/02 ,  C12P17/16 ,  C12P17/10 ,  C12P11/00

Abstract: A Bradyrhizobium monooxygenase, a gene for encoding the monooxygenase, a recombinant expression vector comprising the gene and a recombinant transformant, a method of preparing the monooxygenase by the recombinant expression transformant, and a method of preparing an optically pure chiral sulfoxide by the monooxygenase, in particular to a method of preparing prazole drugs by means of catalyzing the asymmetric oxidation of thioether, a prazole precursor. As compared with other methods of preparing an optically pure sulfoxide, the product produced by the monooxygenase of the present invention as a catalyst has high optical purity, avoids the generation of the byproduct sulfone, and has advantages of mild reaction conditions, simple and convenient operations, easy amplification, etc.

公开(公告)号：US20190274299A1

公开(公告)日：2019-09-12

申请号：US16347429

申请日：2017-11-22

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Meijin Guo ,  Jiarui Li ,  Zejian Wang ,  Yuxia Chen ,  Yingping Zhuang

IPC: A01N1/02 ,  G01N27/22

Abstract: The present disclosure relates to the application of a viable cell monitor to the cryopreservation of plant cells. The viable cell monitor is, for the first time, used in a method for rapidly evaluating the effect of cell freezing-preservation. The effect of a cryoprotectant on cell preservation is evaluated through the change in the capacitance of viable cells before and after cell freezing and resuscitation. Viable cell electrodes can provide a quick and accurate characterization of viable cell concentrations. Combining the viable cell capacitance with the detection result of 2,3,4-triphenyl tetrazolium chloride (TTC), a rapid and quantitative evaluation of the effect of a freezing method on cell preservation can be achieved, thus overcoming the low effectiveness and non-quantification of previous cell preservation methods. Compared with traditional methods, the method of the present disclosure can improve the comprehensive score of cell cryopreservation by 5 times or more.

公开(公告)号：US20190241584A1

公开(公告)日：2019-08-08

申请号：US16318762

申请日：2017-07-18

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Linyong Zhu ,  Yi Yang ,  Dasheng Zhang ,  Zengmin Du ,  Bingkun Bao ,  Qiuning Lin ,  Yan Li ,  Xianjun Chen ,  Lipeng Yang ,  Chunyan Bao

IPC: C07D495/04 ,  C07D333/78 ,  C07D495/14 ,  C09B57/00 ,  G01N33/68 ,  C12Q1/68 ,  G01N33/533 ,  C09B23/04 ,  C09B23/01

CPC classification number: C07D495/04 ,  A61K49/00 ,  C07D333/60 ,  C07D333/78 ,  C07D495/14 ,  C09B23/0058 ,  C09B23/0091 ,  C09B23/04 ,  C09B57/00 ,  C09K11/06 ,  C12Q1/68 ,  G01N33/533 ,  G01N33/68

Abstract: Provided are a fluorescent dye and a preparation process and use thereof. The fluorescent dye is able to emit a long wavelength, is sensitive and specific to viscosity, and is used for a wide range of purposes, such as viscosity testing, fluorescent labeling, quantification or detection of proteins, enzymes or nucleic acids. In addition, it can be used as a fluorescent-activated probe.

公开(公告)号：US10351891B2

公开(公告)日：2019-07-16

申请号：US15535274

申请日：2014-12-12

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Fengqing Wang ,  Dongzhi Wei ,  Liqin Xu ,  Haohao Liu ,  Xinbin Liu ,  Nenghui Chen

IPC: C12P33/02 ,  C12P33/00 ,  C12N9/10 ,  C12N9/04 ,  C12R1/32

Abstract: The present invention provides a hydroxyacyl-coenzyme A dehydrogenase gene, an acyl-coenzyme A thiolase gene, genetically engineered strains and a use thereof. The hydroxyacyl-coenzyme A dehydrogenase gene encodes a protein (i) or (ii) as follows: (i) having an amino acid sequence according to SEQ ID NO 2; (ii) derived by substituting, deleting or inserting one or more amino acids in the amino acid sequence defined by (i) and having the same function as that of the protein of (i). The present invention constructs genetically engineered Mycobacterium strains lacking of a hydroxyacyl-coenzyme A dehydrogenase gene or an acyl-coenzyme A thiolase gene, which are used in the preparation of steroidal compounds, such as 1,4-BNA, 4-BNA, 9-OH-BNA, etc. Further, the invention improves the production efficiency and product quality of steroidal drug, improves the utilization of drug precursors, reduces the production costs, and provides the advantages of mild reaction conditions, environmentally friendly, and high economic and social benefits.

公开(公告)号：US10071910B2

公开(公告)日：2018-09-11

申请号：US15033002

申请日：2013-10-31

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Chongjun Zhao ,  Jianbo Dong ,  Youfu Huang ,  Jingwei Ma ,  Xiuzhen Qian

IPC: C01B31/02 ,  C01B31/00 ,  B01J13/00

CPC classification number: C01B32/15 ,  B01J13/0052 ,  C01B32/00 ,  C01B32/05 ,  C01P2004/64 ,  C01P2006/12 ,  C01P2006/16

Abstract: Methods described herein generally relate to producing carbon aerogel. The method may include providing a carbon-containing polymeric material, and contacting the carbon-containing polymeric material with light, heat or both to produce the carbon aerogel. Systems and kits for producing carbon aerogel are also disclosed.

公开(公告)号：US20180087082A1

公开(公告)日：2018-03-29

申请号：US15535274

申请日：2014-12-12

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： FENGQING WANG ,  DONGZHI WEI ,  LIQIN XU ,  HAOHAO LIU ,  XINBIN LIU ,  NENGHUI CHEN

IPC: C12P33/02 ,  C12N9/04 ,  C12N9/10

CPC classification number: C12P33/02 ,  C12N9/0006 ,  C12N9/10 ,  C12N9/1029 ,  C12P33/00 ,  C12R1/32 ,  C12Y101/01035 ,  C12Y203/01009

Abstract: The present invention provides a hydroxyacyl-coenzyme A dehydrogenase gene, an acyl-coenzyme A thiolase gene, genetically engineered strains and a use thereof. The hydroxyacyl-coenzyme A dehydrogenase gene encodes a protein (i) or (ii) as follows: (i) having an amino acid sequence according to SEQ ID NO 2; (ii) derived by substituting, deleting or inserting one or more amino acids in the amino acid sequence defined by (i) and having the same function as that of the protein of (i). The present invention constructs genetically engineered Mycobacterium strains lacking of a hydroxyacyl-coenzyme A dehydrogenase gene or an acyl-coenzyme A thiolase gene, which are used in the preparation of steroidal compounds, such as 1,4-BNA, 4-BNA, 9-OH-BNA, etc . . . Further, the invention improves the production efficiency and product quality of steroidal drug, improves the utilization of drug precursors, reduces the production costs, and provides the advantages of mild reaction conditions, environmentally friendly, and high economic and social benefits.

公开(公告)号：US20180067076A1

公开(公告)日：2018-03-08

申请号：US15546671

申请日：2016-01-08

Applicant: EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Yitao LONG ,  Chan CAO ,  Yongxu HU

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487

Abstract: A preparation method for an aerolysin nanopore in this disclosure comprises the following steps; (1) pretreatment of an aerolysin; (2) preparation of a lipid bilayer membrane by pulling process; (3) forming of the aerolysin nanopore: the aerolysin nanopore is obtained at a current of 50±5 pA. The aerolysin nanopore prepared in the invention is structurally stable and has a high resolution with the whole internal cavity carried with a positive charge, can be used for detection without modification and is easily operated. Further, the aerolysin nanopore can be applied in DNA sequencing, DNA damage and Micro-RNA detection.

公开(公告)号：US20170349677A1

公开(公告)日：2017-12-07

申请号：US15535902

申请日：2015-11-25

Applicant: East China University Of Science And Technology

Inventor： Boping LIU ,  Jingwen WANG ,  Ruihua CHENG ,  Xuelian HE ,  Zhen LIU ,  Ning ZHAO

IPC: C08F10/02

CPC classification number: C08F10/02 ,  C08F110/02 ,  C08F210/16 ,  C08F4/685 ,  C08F4/646 ,  C08F4/02 ,  C08F210/14

Abstract: The present invention relates to a supported polymetal olefin polymerization catalyst, comprising a porous support, a magnesium-containing support component, a transition metal titanium component supported on the porous support, and further comprising at least one non-magnesium metal component supported on the porous support. Further provided is a preparation method and a use of the supported polymetal olefin polymerization catalyst. An efficient composite support supported polymetal Ziegler-Natta catalyst is provided in the present invention, wherein a porous support, a soluble magnesium compound, and a soluble non-magnesium metal compound are used as raw materials. The supporting of titanium is achieved while a composite support containing magnesium and non-magnesium metal components is formed in situ in the surface of the porous support. The present invention has the advantage of a simple preparation method, a low cost, a controllability of morphology, properties of the catalyst, etc. Comparing the provided catalyst with the same type of magnesium/titanium catalyst free of non-magnesium metal components, the catalytic performance such as polymerzation activity, hydrogen regulation sensitivity and copolymerization performance are significantly improved.

公开(公告)号：US20170088441A1

公开(公告)日：2017-03-30

申请号：US15312190

申请日：2014-07-21

Applicant: East China University of Science and Technology

Inventor： Qiang YANG ,  Hao LU ,  Jiabin ZHU ,  Xiao XU ,  Yiqian LIU

IPC: C02F1/40

CPC classification number: C02F1/40 ,  C02F1/006 ,  C02F1/288 ,  C02F2101/32 ,  C02F2103/10 ,  C02F2103/365

Abstract: The present invention relates to a method and a device for deep oil removal from wastewater containing a low concentration of wasteoil. Wastewater containing a low concentration of wasteoil enters the device via an inlet and passes through a flow conditioner, causing the fluid to become uniformly distributed. Then, by means of a layer of oleophilic-hydrophobic fibers and hydrophilic-oleophobic fibers woven in a certain manner, a trace of oil droplets are captured and then coalesce and grow on the layer, and a trace of oil-in-water emulsion is demulsified and separated on the layer. Finally, by means of corrugation-enhanced sedimentation and separation, the oil droplets coalesce and grow and are then separated rapidly. The invention also provides a set of devices for implementing the method, having several parts such as a housing, a feed pipe, a flow conditioner, a fiber coalescence layer, a corrugation-enhanced separation layer, and a level gauge. The present technique is highly efficient in separation, consumes little power, and can operate continuously for a long period of time. Thus, this technique can be widely used in processes for treating wastewater containing a low concentration of wasteoil.