公开(公告)号：US20210123098A1

公开(公告)日：2021-04-29

申请号：US17144945

申请日：2021-01-08

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Michael PREVITE ,  Molly HE ,  Junhua ZHAO ,  Hui Zhen MAH ,  Chunhong ZHOU ,  Sinan ARSLAN ,  Matthew KELLINGER ,  Lorenzo BERTI ,  Steve CHEN

IPC: C12Q1/6869 ,  C12Q1/6841

Abstract: Provided are methods and systems for analyzing nucleic acids in a biological sample in a manner that retains the spatial and/or cellular origin of the nucleic acids within the biological sample. Compositions and kits are also provided that enable the methods and systems of the instant disclosure.

公开(公告)号：US10982280B2

公开(公告)日：2021-04-20

申请号：US16855877

申请日：2020-04-22

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Sinan Arslan ,  Chunhong Zhou ,  Molly Min He ,  Matthew Kellinger ,  Adeline Huizhen Mah ,  Michael Previte ,  Lei Sun

IPC: C12Q1/68 ,  C07H21/00 ,  C12Q1/6876 ,  G01N33/543 ,  C12N9/12 ,  G01N21/64

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

公开(公告)号：US20200347443A1

公开(公告)日：2020-11-05

申请号：US16543351

申请日：2019-08-16

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Michael PREVITE

IPC: C12Q1/6834 ,  C12Q1/6832 ,  C12Q1/6806 ,  B01J19/00

Abstract: Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one organic solvent having a dielectric constant in the range of no greater than 115; and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.

公开(公告)号：US20250027144A1

公开(公告)日：2025-01-23

申请号：US18415517

申请日：2024-01-17

Applicant: Element Biosciences, Inc.

Inventor： Sinan ARSLAN ,  Molly HE ,  Michael PREVITE

IPC: C12Q1/6869 ,  G01N15/1434 ,  G01N21/64

Abstract: Nucleic acid hybridization buffer formulations and uses thereof are described that yield improvements in hybridization specificity, rate, and efficiency. The buffer formulation composition includes a target nucleic acid; at least one polar, aprotic, organic solvent, and a pH buffer system, wherein the target nucleic acid is attached to the surface via hybridization to a surface bound nucleic acid tethered to the surface, and wherein the hybridization of the target nucleic acid and surface bound nucleic acid has a high stringency and annealing rate.

公开(公告)号：US20240382956A1

公开(公告)日：2024-11-21

申请号：US18680203

申请日：2024-05-31

Applicant: Element Biosciences, Inc.

Inventor： Minghao GUO ,  Leon Zilun ZHANG ,  Chunhong ZHOU ,  Matthew KELLINGER ,  Michael PREVITE

IPC: B01L3/00

Abstract: Flow cell devices, cartridges, and systems are described that provide reduced manufacturing complexity, lowered consumable costs, and flexible system throughput for nucleic acid sequencing and other chemical or biological analysis applications. The flow cell device can include a capillary flow cell device or a microfluidic flow cell device.

公开(公告)号：US12091657B2

公开(公告)日：2024-09-17

申请号：US17120030

申请日：2020-12-11

Applicant: ELEMENT BIOSCIENCES, INC.

Inventor： Matthew Kellinger ,  Molly He ,  Tyler Lopez

IPC: C12N15/10 ,  C12N9/12 ,  C12P21/00 ,  C12Q1/6874

CPC classification number: C12N15/102 ,  C12N9/1276 ,  C12N15/1068 ,  C12Q1/6874 ,  C12Y207/07049 ,  C12N2310/321 ,  C12P21/00 ,  C12Q1/6874 ,  C12Q2521/107 ,  C12Q2525/186

Abstract: Provided herein are compositions and methods for the incorporation of unnatural nucleotides using mutant polymerases, such as reverse transcriptases. Further provided herein are methods of detection and sequencing of polynucleotide sequences. In some aspects, the compositions and methods are used enhance the efficiency and speed of detecting nucleotide bases. The methods and compositions described herein may further reduce time, cost, or scale of devices for next generation sequencing platforms.

公开(公告)号：US20240287482A1

公开(公告)日：2024-08-29

申请号：US18650856

申请日：2024-04-30

Applicant: Element Biosciences, Inc.

Inventor： Jendrik HENTSCHEL ,  Tyler LOPEZ ,  Michael KLEIN ,  Virginia SAADE ,  Matthew KELLINGER ,  Mark AMBROSO

IPC: C12N9/12 ,  C12Q1/6869

CPC classification number: C12N9/1252 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: Provided herein are engineered variants of archaeal polymerases that exhibit exonuclease-minus activity, enhanced thermostability, enhanced incorporation of 3′ modified nucleotides, improved uracil-tolerance and/or reduce sequence-specific errors in polymerase-catalyzed nucleotide binding and extension reactions relative to wild type polymerase enzymes. Also provided are uses of the engineered polymerases for forming complexed polymerases and forming binding complexes, and uses for conducting nucleic acid sequencing reactions.

公开(公告)号：US20240240249A1

公开(公告)日：2024-07-18

申请号：US18424417

申请日：2024-01-26

Applicant: Element Biosciences, Inc.

Inventor： Genhua ZHENG ,  Njoo Audrey WIBAWA ,  Xi ZHANG ,  Zhimin YANG ,  Marcus James JELLEN ,  Gene SHEN

IPC: C12Q1/6874 ,  C09B23/06 ,  C09B23/10 ,  C12Q1/48

CPC classification number: C12Q1/6874 ,  C09B23/06 ,  C09B23/105 ,  C12Q1/485 ,  G01N2333/91245

Abstract: The present disclosure provides a compound of Formula (I), (II), or (III):




an ionic derivative thereof, an isomer thereof, or a salt thereof. The present disclosure also provides conjugates of the compounds, and methods of using the compounds and the conjugates. The disclosure also provides the use of the compounds and conjugates in methods of sequencing nucleic acids.

公开(公告)号：US20230326064A1

公开(公告)日：2023-10-12

申请号：US18078797

申请日：2022-12-09

Applicant: Element Biosciences, Inc.

Inventor： Chunhong ZHOU ,  Semyon KRUGLYAK ,  Francisco GARCIA ,  Minghao GUO ,  Haosen WANG ,  Ryan KELLY

IPC: G06T7/66 ,  G06T7/30 ,  G06V10/75 ,  G06V20/69 ,  G06F18/232 ,  G06V10/762

CPC classification number: G06T7/66 ,  G06T7/30 ,  G06V10/751 ,  G06V20/693 ,  G06V20/695 ,  G06F18/232 ,  G06V10/763 ,  G06V20/69 ,  G06V2201/04

Abstract: Image data analysis, particularly identifying cluster locations for performing base-calling in a digital flow cell image during DNA sequencing, is described. Each nucleic acid template molecule immobilized on a support may include an insert sequence and a sample index sequence. The sample index sequence may include a k-mer sequence. A sequencing system may conduct k cycles of sequencing reactions of the k-mer sequence before conducting one or more cycles of the insert sequence sequencing reactions and generate a first plurality of flow cell images. Pixel intensities may be determined for pixels of the first plurality of flow cell images. A base calling template may be determined and include base calling locations based on the pixel intensities and respective color purities of the pixel intensities. The base calling template may register a second plurality of flow cell images of the support in one or more cycles subsequent to the k cycles.

公开(公告)号：US20230323450A1

公开(公告)日：2023-10-12

申请号：US18181476

申请日：2023-03-09

Applicant: Element Biosciences, Inc.

Inventor： Sinan Arslan ,  Molly Min HE ,  Matthew KELLINGER ,  Jake LEVIEUX ,  Michael PREVITE ,  Junhua ZHAO ,  Su ZHANG ,  Tyler LOPEZ

IPC: C12Q1/6874 ,  G01N33/58 ,  G01N33/53

CPC classification number: C12Q1/6874 ,  G01N33/582 ,  G01N33/5308

Abstract: Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.