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公开(公告)号：US20220118440A1
公开(公告)日：2022-04-21
申请号：US17072847
申请日：2020-10-16
Inventor： Arkadiusz Pajak , Sebastian Purmann , Jeffrey Steaffens , Neil Jones , Andrew Geddes , Julien Oudia
Abstract: A sample vial for delivery of fluid sample to a flow cytometer includes a fluid-containment cavity with a tapered portion in which the cavity cross-section tapers in a distal direction toward the bottom of the cavity, and the sample vial has a ribbed exterior portion with longitudinally extending ribs and recesses between the ribs. An array of the sample vials can be retained in receptacles of a processing tray and are limited in rotation relative to the tray by a rotational stop feature in the receptacles that engages with the ribbed exterior portion.
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22.发明申请公开(公告)号：US20210325309A1
公开(公告)日：2021-10-21
申请号：US16854756
申请日：2020-04-21
Inventor： Bradley Neagle , Alan Riggs , Michael Kusner , Kyle Schutte , Eric Endsley
Abstract: An imaging apparatus is provided to facilitate epifluorescent imaging of three (or more) color channels and to perform phase contrast and/or bright field imaging of samples without manual adjustment of the imaging apparatus. This allows for automated imaging, over extended periods of time, of a plurality of samples by a device located inside an incubator without disturbing the incubator environment to manually adjust the apparatus. Also provided are embodiments to facilitate user swapping of removable optical modules and/or transillumination modules to allow the imaging apparatus to be adapted to different combinations of assays and/or fluorescent indicators so as to increase the variety of experiments and/or fluorescent dyes that can be imaged using the imaging apparatus.
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公开(公告)号：US20200250822A1
公开(公告)日：2020-08-06
申请号：US16265910
申请日：2019-02-01
Inventor： Timothy R. Jackson , Nevine Holtz
Abstract: The disclosure provides example methods that include a processor: (a) generating at least one phase contrast image of a biological specimen comprising one or more cells centered around a focal plane for the biological specimen; (b) generating a confluence mask in the form of a binary image based on the at least one phase contrast image; (c) receiving a first brightfield image of the biological specimen at a defocusing distance above the focal plane and a second brightfield image of the biological specimen at the defocusing distance below the focal plane; (d) generating a cell image of the biological specimen based on the first and second brightfield image; (e) generating a seed mask based on the cell image and the phase contrast image; and (f) generating an image of the biological specimen showing a cell-by-cell segmentation mask based on the seed mask and the confluence mask.
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公开(公告)号：US10732089B2
公开(公告)日：2020-08-04
申请号：US16049899
申请日：2018-07-31
Applicant: Essen Instruments, Inc.
Inventor： Aaron B. Kennington
Abstract: A flow cytometer apparatus and methods for detecting and clearing a clog therein are disclosed. An example method for detecting a clog may include (i) detecting, via a fault detection system of a flow cytometer, a first plurality of events associated with a first aliquot from a first sample well, (ii) determining a count of the first plurality of events associated with the first aliquot, (iii) determining whether the count of the first plurality of events is below a minimum count tolerance and (iv) (a) if the count of the first plurality of events is below the minimum count tolerance, then determining that the flow cytometer has a clog, (b) if the count of the first plurality of events is equal to or above the minimum count tolerance, then detecting a second plurality of events associated with a second aliquot from a second sample well.
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公开(公告)号：US20200224139A1
公开(公告)日：2020-07-16
申请号：US16827609
申请日：2020-03-23
Applicant: ESSEN INSTRUMENTS, INC. D/B/A ESSEN BIOSCIENCE, INC. , NATIONAL RESEARCH COUNCIL OF CANADA
Inventor： Kirk S. SCHROEDER , Bradley D. NEAGLE , Eric ENDSLEY , Daniel APPLEDORN , Keith MORTON
Abstract: Systems, methods, and apparatuses of controlling fluid flow are disclosed. An apparatus includes a first microplate having a first open portion and defining one or more first wells therein, a second microplate having a second open portion and defining one or more second wells therein, and a pneumatic lid constructed of styrene ethylene butylene styrene (SEBS). The pneumatic lid extends over the first open portion and the second open portion and includes one or more microfluidic channels that fluidly couple the one or more first wells to the one or more second wells. The pneumatic lid provides an airtight seal over the first microplate and the second microplate.
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Patent Agency Ranking
公开(公告)号：US10705007B2
公开(公告)日：2020-07-07
申请号：US16040168
申请日：2018-07-19
Applicant: Essen Instruments, Inc.
Inventor： Kathy L. Rowlen , Matthew Ferris
Abstract: A flow cytometry system and method for quantification of fluorescently labeled virus particles smaller than 1 micron in size measures and controls sample fluid flow rate to an investigatory flow cell at a rate below 5000 nanoliters per minute and calculates in real time particle concentration in the sample fluid using measured flow rated data flow rate data and with correction for sample fluid flow rate fluctuations.
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公开(公告)号：US10408734B2
公开(公告)日：2019-09-10
申请号：US16215153
申请日：2018-12-10
Applicant: Essen Instruments, Inc.
Abstract: A method for evaluating a biological material for unassociated virus-size particles having a particular epitope uses a fluorescent antibody stain specific for binding with the epitope and a fluid sample with the virus-size particles and fluorescent antibody stain is subjected to flow cytometry with identification of fluorescent emission detection events indicative of passage through a flow cell of a flow cytometer of unassociated labeled particles of virus size including such a virus-size particle and fluorescent antibody stain.
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公开(公告)号：US20220146485A1
公开(公告)日：2022-05-12
申请号：US17096681
申请日：2020-11-12
Inventor： Michael W. Olszowy
Abstract: Fluorescent viral particles, including viral particles and a fluorescent dye conjugated to the viral particles, are used for viral contaminant removal testing as part of viral clearance evaluation for biological medical product preparation processes. The conjugated fluorescent dye adds only marginally to viral particle size, but provides versatility both for rapid screening of alternative viral removal approaches during process development and for final process validation, as the fluorescent viral particles have fluorescent activity throughout the viral clearance evaluation process, permitting rapid and consistent quantification of initial test solutions, resulting purified solutions, and intermediate solutions of interest at any point during processing under evaluation.
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29.发明申请公开(公告)号：US20220026699A1
公开(公告)日：2022-01-27
申请号：US16935326
申请日：2020-07-22
Inventor： Timothy JACKSON , Rickard SJÖGREN , Christoffer EDLUND , Edvin FORSGREN
IPC: G02B21/36 , H04N5/235 , G06T7/571 , G06T15/08 , G06N3/08 , G06N3/04 , G01N21/64 , G02B21/16 , G02B21/34 , G01N33/50 , C12N5/09 , C12N5/071
Abstract: A method is disclosed for acquiring a single, in-focus two-dimensional projection image of a live, three-dimensional cell culture sample, with a fluorescence microscope. One or more long-exposure “Z-sweep” images are obtained, i.e. via a single or series of continuous acquisitions, while moving the Z-focal plane of a camera through the sample, to produce one or more two-dimensional images of fluorescence intensity integrated over the Z-dimension. The acquisition method is much faster than a Z-stack method, which enables higher throughput and reduces the risk of exposing the sample to too much fluorescent light. The long-exposure Z-sweep image(s) is then input into a neural network which has been trained to produce a high-quality (in-focus) two-dimensional projection image of the sample. With these high-quality projection images, biologically relevant analysis metrics can be obtained to describe the fluorescence signal using standard image analysis techniques, such as fluorescence object count and other fluorescence intensity metrics (e.g., mean intensity, texture, etc.).
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公开(公告)号：US11137336B2
公开(公告)日：2021-10-05
申请号：US16175963
申请日：2018-10-31
Applicant: Essen Instruments, Inc.
Inventor： Stephen Barnes
Abstract: A flow cytometer module configured to be integrated with a liquid handling system is provided herein. The flow cytometer module includes (a) a flow cell, (b) a first fluidic pathway, (c) an inlet configured to receive a sample introduction device of the liquid handling system including one or more samples, (d) a second fluidic pathway in fluid communication with the first fluidic pathway, (e) a laser interrogation device configured to examine the one or more samples at a laser interrogation point in the second fluidic pathway, and (f) a controller in communication with the liquid handling system and configured to cause the flow cytometer module to perform functions comprising: (i) recording data from the laser interrogation device corresponding to a plurality of events as the one or more samples pass the laser interrogation point, and (ii) transmitting the data corresponding to the plurality of events to the liquid handling system.
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