公开(公告)号：US20110281291A1

公开(公告)日：2011-11-17

申请号：US12800379

申请日：2010-05-14

Applicant: Michael Ullery ,  Erin Mathias ,  Jones M. Hyman ,  John D. Walsh

Inventor： Michael Ullery ,  Erin Mathias ,  Jones M. Hyman ,  John D. Walsh

IPC: C12Q1/04 ,  G01J3/44 ,  H01J49/26 ,  G01N33/48

CPC classification number: C12Q1/04 ,  G01N21/6486 ,  G06F19/24

Abstract: A method for identification and/or characterization of a microbial agent present in a sample includes a step of analytical test data (e.g., obtaining intrinsic fluorescence values over a range of emission wavelengths) from the microbial agent. The analytical test data is transformed thereby minimizing strain to strain variations within an organism group. With the aid of a programmed computer, a multi-level classification algorithm coded as a set of processing instructions operates on the transformed analytic test data. The multiple levels correspond to different levels in a taxonomic hierarchy for microbial agents suspected of being in the sample.

Abstract translation: 用于鉴定和/或表征样品中存在的微生物剂的方法包括从微生物剂中分析测试数据（例如，在发射波长范围内获得固有荧光值）的步骤。 转化分析试验数据，从而使生物体组内的应变变化最小化。 借助编程的计算机，编码为一组处理指令的多级分类算法对转换的分析测试数据进行操作。 多个级别对应于涉嫌在样品中的微生物剂的分类层级中的不同水平。

公开(公告)号：US20110033847A1

公开(公告)日：2011-02-10

申请号：US12653527

申请日：2009-12-15

Applicant: John Walsh ,  Jones M. Hyman ,  Thurman Thorpe

Inventor： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe

IPC: C12Q1/04 ,  C12Q1/68 ,  G01N33/566

CPC classification number: C12Q1/04

Abstract: The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

Abstract translation: 本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。 所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

公开(公告)号：US20100291618A1

公开(公告)日：2010-11-18

申请号：US12800387

申请日：2010-05-14

Applicant: Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman

Inventor： Ronnie J. Robinson ,  Mark S. Wilson ,  Christopher S. Ronsick ,  John D. Walsh ,  Jones M. Hyman

IPC: C12Q1/04 ,  C12Q1/02 ,  C12N1/00

CPC classification number: C12Q1/24 ,  G01N35/0099 ,  G01N35/1079 ,  Y10S901/30

Abstract: A method of testing a specimen sample contained within a sealed specimen container. A test sample is removed from the specimen container with a disposable sampling device. Non-microbial agent components of the test sample are lysed, thereby producing a lysed sample. The lysed sample is delivered to a disposable separation device. A microbial agent present in test sample is concentrated within the disposable separation device. The concentrated microbial agent is interrogated, e.g., spectroscopically.

Abstract translation: 一种测试包含在密封样品容器内的样品样品的方法。 用一次性取样装置将样品从样品容器中取出。 裂解试验样品的非微生物剂组分，从而产生裂解的样品。 裂解的样品被输送到一次性分离装置。 存在于试样中的微生物剂集中在一次性分离装置内。 浓缩的微生物剂被询问，例如光谱法。

公开(公告)号：US20100124763A1

公开(公告)日：2010-05-20

申请号：US12589968

申请日：2009-10-30

Applicant: John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

Inventor： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC: C12Q1/04

CPC classification number: C12Q1/04 ,  G01N21/35 ,  G01N21/3577 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

Abstract: The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.

Abstract translation: 本发明提供了用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统。 该方法可以在密封容器中通过利用第一步骤时间依赖性光谱技术来实现，以获得包含样品的生长组合物的至少两个测量值，并将所述测量结果与生物学颗粒的检测和/或表征相关联，生物颗粒可以是 存在于样品中。 该方法还提供了在密封容器中分离，表征和/或鉴定微生物的后续步骤。

公开(公告)号：US20100120133A1

公开(公告)日：2010-05-13

申请号：US12589969

申请日：2009-10-30

Applicant: John Walsh ,  Jones M. Hyman ,  Christopher Ronsick ,  John Link ,  Ron Robinson ,  Mark Wilson

Inventor： John Walsh ,  Jones M. Hyman ,  Christopher Ronsick ,  John Link ,  Ron Robinson ,  Mark Wilson

IPC: C12M1/34

CPC classification number: C12Q1/24 ,  B01L3/5021 ,  B01L2300/042 ,  B01L2300/0838 ,  B01L2300/0861 ,  C12Q1/04 ,  C12Q1/37 ,  C12Q1/6816 ,  G01N21/03 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

Abstract: The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein.

Abstract translation: 本发明涉及可用于从已知含有或怀疑含有所述微生物的测试样品中分离，分离或造粒微生物的分离装置或容器。 随后，分离的，分离的或沉淀的微生物样品可以经历一个或多个询问步骤以提供对微生物的表征和/或鉴定有用的测量。 在本发明的一个方面，询问步骤可以在本文所述的分离装置或容器中原位发生。

公开(公告)号：US20100068755A1

公开(公告)日：2010-03-18

申请号：US12460607

申请日：2009-07-22

Applicant: John D. Walsh ,  Jones M. Hyman ,  Bradford G. Clay ,  Thurman C. Thorpe

Inventor： John D. Walsh ,  Jones M. Hyman ,  Bradford G. Clay ,  Thurman C. Thorpe

IPC: C12Q1/04 ,  C12M1/34

CPC classification number: C12Q1/04 ,  C12Q1/10 ,  G01N21/31 ,  G01N21/47 ,  G01N21/64 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample whereby the method may be accomplished non-invasively by utilizing a time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle, that may be present in the sample.

Abstract translation: 本发明提供了一种用于监测，检测和/或表征可能存在于样品中的生物颗粒的方法和系统，其中所述方法可以非侵入地通过利用时间依赖的光谱技术来获得至少两个测量 的生长组合物，其包含样品并将所述测量结果与可能存在于样品中的生物颗粒的检测和/或表征相关联。

公开(公告)号：US5976827A

公开(公告)日：1999-11-02

申请号：US989560

申请日：1997-12-12

Applicant: Scott R. Jeffrey ,  Paul M. Matsumura ,  Martin J. Maresch ,  Jones M. Hyman ,  Thurman C. Thorpe

Inventor： Scott R. Jeffrey ,  Paul M. Matsumura ,  Martin J. Maresch ,  Jones M. Hyman ,  Thurman C. Thorpe

IPC: C12M1/28 ,  C12M1/34 ,  C12Q1/04 ,  C12Q1/24

CPC classification number: C12Q1/04 ,  C12M23/04 ,  C12M23/22 ,  C12M23/24 ,  C12M25/06 ,  C12M25/14 ,  C12Q1/045 ,  C12Q1/24

Abstract: A device and method allow for detecting the presence of microorganisms in clinical and non-clinical specimens. The device, a sensor, provides an environment to culture microbial organism colonies from a fluid sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The sensor has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. The sensor provides an area for accepting a fluid sample, a mechanism to immobilize the fluid sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor for allowing the detection and/or enumeration of microorganism colonies within the sample.

Abstract translation: 一种装置和方法允许检测临床和非临床标本中微生物的存在。 该装置，传感器，提供了从流体样品培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。 传感器具有微生物固定基质层和传感器层。 检测到的微生物菌落立即可用于进一步测试。 传感器提供用于接受流体样品的区域，将流体样品固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及用于允许微生物菌落的检测和/或计数的传感器 在样品内。

公开(公告)号：US5912115A

公开(公告)日：1999-06-15

申请号：US113929

申请日：1998-07-10

Applicant: Jones M. Hyman ,  Thurman C. Thorpe

Inventor： Jones M. Hyman ,  Thurman C. Thorpe

IPC: C12M1/00 ,  C12M1/28 ,  C12M1/34 ,  C12Q1/02 ,  C12Q1/04 ,  C12Q1/24 ,  C12Q1/00

CPC classification number: C12M23/04 ,  C12M23/22 ,  C12M23/24 ,  C12M25/06 ,  C12M25/14 ,  C12M41/36 ,  C12Q1/04 ,  C12Q1/045 ,  C12Q1/24

Abstract: A device and method allow for collection of a blood specimen and for detecting the presence of microorganisms in the blood specimen. The device, a sensor plate under vacuum, provides an environment to culture microbial organism colonies from a blood sample, and a means to facilitate microbial detection and quantification, either manually or with an instrument. The vacuum sensor plate has a microorganism immobilization matrix layer and a sensor layer. Detected microbial colonies are immediately available for further testing. A kit is provided having a needle assembly for direct draw of blood from a patient onto the immobilization layer within the vacuum sensor plate. The sensor plate provides an area for accepting a blood sample, a mechanism to immobilize the blood sample on an interior surface of the plate, nutrients to facilitate growth of microorganisms in the sample, and a sensor layer for allowing the detection and/or enumeration of microorganism colonies within the blood sample.

Abstract translation: 装置和方法允许收集血液样本并检测血液样本中微生物的存在。 该装置是真空下的传感器板，提供了从血液样品中培养微生物菌落的环境，以及手动或用仪器促进微生物检测和定量的手段。 真空传感器板具有微生物固定基质层和传感器层。 检测到的微生物菌落立即可用于进一步测试。 提供了一种套件，其具有用于将血液从患者直接抽吸到真空传感器板内的固定层上的针组件。 传感器板提供用于接受血液样本的区域，将血液样本固定在板的内表面上的机制，营养物质以促进样品中微生物的生长，以及传感器层，用于允许检测和/或计数 血液样品中的微生物菌落。

公开(公告)号：US5384240A

公开(公告)日：1995-01-24

申请号：US981689

申请日：1992-11-25

Applicant: Jones M. Hyman

Inventor： Jones M. Hyman

IPC: G01N33/531 ,  G01N33/53 ,  G01N33/536 ,  G01N33/569 ,  G01N33/574 ,  C12Q1/70

CPC classification number: G01N33/5306 ,  G01N33/536 ,  G01N33/56988 ,  Y10S435/974 ,  Y10S436/82 ,  Y10S436/825 ,  Y10S436/826

Abstract: This invention relates to a novel reagent and a method of using the reagent in an immunoassay to detect antigens, particularly antigens immunocomplexed with their corresponding or cross-reacting antibodies. In particular, this reagent and method increase the detection of human immunodeficiency virus HIV-1 p24 core antigen.

Abstract translation: 本发明涉及一种新的试剂和在免疫测定中使用该试剂的方法来检测抗原，特别是与其相应或交叉反应抗体免疫复合的抗原。 特别地，该试剂和方法增加了人体免疫缺陷病毒HIV-1 p24核心抗原的检测。

公开(公告)号：US3758436A

公开(公告)日：1973-09-11

申请号：US23048172

申请日：1972-02-29

Applicant: JONES M ,  JENNINGS B

Inventor： JONES M ,  JENNINGS B

IPC: C08G61/12 ,  C08G75/20 ,  C08G51/10

CPC classification number: C08G75/20 ,  C08G61/12

Abstract: A CROSS-LINKABLE COMPOSITION WHICH COMPRISES A THERMOPLASTIC POLYMER CONTAINING AROMATIC RESIDUES IN THE POLYMER CHAIN, THE POLYMER ISELF CONTAINING PER CHAIN AT LEAST TWO GROUPS OF THE FORMULA -SO2X EACH ATTACHED DIRECTLY TO AN AROMATIC CARBON ATOM, X BEING, FOR EXAMPLE, CHLORINE OR BROMINE.