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    Method for separation, characterization and/or identification of microorganisms using spectroscopy
    2.
    发明授权
    Method for separation, characterization and/or identification of microorganisms using spectroscopy 有权
    使用光谱法分离，表征和/或鉴定微生物的方法

    公开(公告)号：US08652800B2

    公开(公告)日：2014-02-18

    申请号：US12589952

    申请日：2009-10-30

    申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    IPC分类号： C12Q1/04

    CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848 ,  Y02A50/55

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

    Method for separation, characterization and/or identification of microorganisms using spectroscopy
    3.
    发明申请
    Method for separation, characterization and/or identification of microorganisms using spectroscopy 有权
    使用光谱法分离，表征和/或鉴定微生物的方法

    公开(公告)号：US20100129858A1

    公开(公告)日：2010-05-27

    申请号：US12589952

    申请日：2009-10-30

    申请人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    发明人： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    IPC分类号： C12Q1/04

    CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848 ,  Y02A50/55

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

    METHODS AND KITS FOR ISOLATING MICROORGANISMS FROM CULTURE
    5.
    发明申请
    METHODS AND KITS FOR ISOLATING MICROORGANISMS FROM CULTURE 审中-公开
    从文化中分离微生物的方法和工具

    公开(公告)号：US20130045532A1

    公开(公告)日：2013-02-21

    申请号：US13554704

    申请日：2012-07-20

    申请人： Jones M. Hyman ,  John D. Walsh ,  Ronnie J. Robinson ,  Christopher Ronsick

    发明人： Jones M. Hyman ,  John D. Walsh ,  Ronnie J. Robinson ,  Christopher Ronsick

    IPC分类号： C12M1/26 ,  C12M1/40

    CPC分类号： C12M47/02 ,  C12N1/02 ,  C12N1/06 ,  C12Q1/24

    摘要： The present invention is directed to methods and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. The kit of the present invention may comprise at least one filter membrane or an integrated filtration and sample transfer device for isolation and/or accumulation of microorganisms.

    摘要翻译： 本发明涉及用于分离，积累，表征和/或鉴定已知存在或可能存在于测试样品中的微生物的方法和试剂盒。 本发明的方法包括任选地裂解可能存在于测试样品中的非微生物细胞和/或颗粒，随后进行用于分离和/或积累微生物的随后的过滤步骤。 本发明的试剂盒可以包括用于分离和/或积累微生物的至少一个过滤膜或一体化过滤和样品转移装置。

    Methods For The Isolation, Accumulation, Characterization And/Or Identification Of Microorganisms Using A Filtration And Sample Transfer Device
    6.
    发明申请
    Methods For The Isolation, Accumulation, Characterization And/Or Identification Of Microorganisms Using A Filtration And Sample Transfer Device 审中-公开
    使用过滤和样品转移装置的微生物的分离，积累，表征和/或鉴定方法

    公开(公告)号：US20120156716A1

    公开(公告)日：2012-06-21

    申请号：US13328379

    申请日：2011-12-16

    申请人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

    发明人： John Walsh ,  Jones Hyman ,  Christopher Ronsick

    IPC分类号： C12Q1/04 ,  C12M1/00 ,  B01D35/00

    CPC分类号： B01L3/50255 ,  B01L3/561 ,  B01L2300/0681

    摘要： The present invention is directed to methods, devises and kits for separating, accumulating, characterizing and/or identifying microorganisms known to be present or that may be present in a test sample. The method of the invention comprises optional lysing non-microorganism cells and/or particulates that may be present in a test sample, followed by a subsequent filtration step for isolation and/or accumulation of microorganisms. In one embodiment, a filtration and sample transfer device is used for isolation and/or accumulation of microorganisms by filtration. Once the microorganisms have been filtered for isolation and/or accumulation microorganisms, the isolated and/or accumulated microorganisms can be analyzing to acquire measurements for the characterization and/or identification of said microorganisms.

    摘要翻译： 本发明涉及用于分离，积累，表征和/或鉴定已知存在或可能存在于测试样品中的微生物的方法，设计和试剂盒。 本发明的方法包括任选地裂解可能存在于测试样品中的非微生物细胞和/或颗粒，随后进行用于分离和/或积累微生物的随后的过滤步骤。 在一个实施方案中，过滤和样品转移装置用于通过过滤分离和/或积累微生物。 一旦微生物已被过滤以分离和/或累积微生物，分离的和/或累积的微生物可以被分析以获得用于表征和/或鉴定所述微生物的测量。

    Methods for Separation, Characterization and/or Identification of Microorganisms using Spectroscopy
    9.
    发明申请
    Methods for Separation, Characterization and/or Identification of Microorganisms using Spectroscopy 有权
    使用光谱分离，表征和/或鉴定微生物的方法

    公开(公告)号：US20140186879A1

    公开(公告)日：2014-07-03

    申请号：US14146367

    申请日：2014-01-02

    申请人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    发明人： John Walsh ,  Jones Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

    IPC分类号： C12Q1/04

    CPC分类号： C12Q1/04 ,  G01N33/6848 ,  H01J49/00

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

    Method for separation, characterization and/or identification of microorganisms using raman spectroscopy
    10.
    发明申请
    Method for separation, characterization and/or identification of microorganisms using raman spectroscopy 审中-公开
    使用拉曼光谱法分离，表征和/或鉴定微生物的方法

    公开(公告)号：US20100136609A1

    公开(公告)日：2010-06-03

    申请号：US12589976

    申请日：2009-10-30

    申请人： Bradford Clay ,  Jones Hyman ,  John Walsh ,  Thurman Thorpe ,  Christopher Ronsick

    发明人： Bradford Clay ,  Jones Hyman ,  John Walsh ,  Thurman Thorpe ,  Christopher Ronsick

    IPC分类号： C12Q1/04 ,  C12Q1/02

    CPC分类号： C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

    摘要： The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for Raman spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said Raman spectroscopic measurements.

    摘要翻译： 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明进一步提供分离的微生物样品的拉曼光谱询问以产生微生物的测量，并使用所述拉曼光谱测量来表征和/或鉴定样品中的微生物。