公开(公告)号：US20140335558A1

公开(公告)日：2014-11-13

申请号：US14444708

申请日：2014-07-28

Applicant: Jones M. Hyman ,  John D. Walsh ,  Thurman C. Thorpe

Inventor： Jones M. Hyman ,  John D. Walsh ,  Thurman C. Thorpe

IPC: C12Q1/04

CPC classification number: C12Q1/04

Abstract: The present invention relates to methods and systems for scanning, detecting, and monitoring microorganisms on solid or semi-solid media using intrinsic fluorescence (IF) measurements. The methods are further directed to detection, characterization and/or identification of microorganisms on a solid or semi-solid media using intrinsic fluorescence (IF) measurements that are characteristic of said microorganisms.

Abstract translation: 本发明涉及使用固有荧光（IF）测量在固体或半固体培养基上扫描，检测和监测微生物的方法和系统。 所述方法进一步涉及使用所述微生物特征的固有荧光（IF）测量法在固体或半固体培养基上检测，表征和/或鉴定微生物。

公开(公告)号：US08735091B2

公开(公告)日：2014-05-27

申请号：US13828119

申请日：2013-03-14

Applicant: Jones M. Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

Inventor： Jones M. Hyman ,  Parampal Deol ,  Elizabeth Miller ,  Victoria Girard ,  Amber Gates ,  Sandrine Mailler ,  Maud Arsac ,  John Walsh

IPC: C12Q1/24

CPC classification number: G01N1/4077 ,  C12Q1/24 ,  G01N33/5695 ,  G01N33/6851 ,  G01N2333/35 ,  G01N2333/355 ,  G01N2560/00

Abstract: The present invention is directed to a method for inactivation and/or extraction of acid-fast bacteria (e.g., mycobacteria or nocardia), the method comprising the following sequential steps: (a) acquiring a test sample known to contain or that may contain acid-fast bacteria and suspending the test sample in a container containing ethanol and beads; (b) bead beating and/or vortexing the container to break up clumps and/or disrupt acid-fast bacteria cells in the container; and (c) subsequently incubating the suspension for at least about 3 minutes at room temperature to inactivate any acid-fast bacteria contained in the test sample. In accordance with the present invention, the test sample can subsequently be pelleted by centrifugation, resuspended with formic acid, acetonitrile added, and subjected to mass spectrometry for characterization and/or identification of the acid-fast bacteria.

Abstract translation: 本发明涉及一种灭活和/或提取酸性抗菌（例如分枝杆菌或诺卡氏菌）的方法，该方法包括以下顺序步骤：（a）获得已知含有或可含有酸的测试样品 将试样悬浮在含有乙醇和珠粒的容器中; （b）珠子打浆和/或涡旋容器以分解块和/或破坏容器中的耐酸细菌细胞; 和（c）随后在室温下将悬浮液温育至少约3分钟以灭活测试样品中所含的任何耐酸细菌。 根据本发明，随后通过离心将测试样品沉淀，用甲酸重悬浮，加入乙腈，并进行质谱鉴定和/或鉴定耐酸细菌。

公开(公告)号：US07429464B2

公开(公告)日：2008-09-30

申请号：US11006025

申请日：2004-12-07

Applicant: John D. Walsh ,  Jones M. Hyman

Inventor： John D. Walsh ,  Jones M. Hyman

IPC: C12Q1/14 ,  C12Q1/04

CPC classification number: G01N33/56916 ,  C12Q1/04 ,  C12Q1/10 ,  C12Q1/14 ,  G01N33/56938

Abstract: The present invention provides for methods for detecting bacterial pathogens in a sample. A preferred method includes the steps of: suspending a sample comprising a medium and microorganisms, the microorganisms suspected of comprising bacterial pathogens; mixing the sample, an adsorbent and a first solution in a vessel; separating the adsorbent and the microorganisms from the medium and the bulk of the first solution and removing the medium and the bulk of the first solution from the vessel; adding a second solution to the vessel to resuspend the adsorbent and the microorganisms; and detecting agglutination of the adsorbent wherein the agglutination of the adsorbent signifies bacterial pathogens are present in the sample.

Abstract translation: 本发明提供了检测样品中细菌病原体的方法。 优选的方法包括以下步骤：将包含培养基和微生物的样品悬浮在怀疑包含细菌病原体的微生物中; 将样品，吸附剂和第一溶液混合在容器中; 从介质和大部分第一溶液中分离吸附剂和微生物，并从容器中除去介质和第一溶液的体积; 向容器中加入第二溶液以重新悬浮吸附剂和微生物; 并检测吸附剂的凝集，其中吸附剂的凝集表示细菌病原体存在于样品中。

公开(公告)号：US5350697A

公开(公告)日：1994-09-27

申请号：US574184

申请日：1990-08-28

Applicant: C. Hermas Swope ,  John G. Link ,  Jones M. Hyman

Inventor： C. Hermas Swope ,  John G. Link ,  Jones M. Hyman

IPC: G01N21/47 ,  G01N33/543 ,  G01N33/552

CPC classification number: G01N21/47 ,  G01N33/54373 ,  G01N2021/4707 ,  G01N2021/4709 ,  Y10S435/808 ,  Y10S436/805

Abstract: An apparatus in which scattered light is measured, said apparatus having a light source aligned to direct illumination toward an interface between a sample container and an aqueous solution at an angle less than the critical angle. A detector to measure the scattered light is located at a place outside the envelope of the critical angle.

Abstract translation: 一种测量散射光的装置，所述装置具有光源，该光源被排列成以小于临界角的角度朝向样品容器和水溶液之间的界面引导照射。 用于测量散射光的检测器位于临界角的包络外的位置。

公开(公告)号：US3817435A

公开(公告)日：1974-06-18

申请号：US23742972

申请日：1972-03-23

Applicant: JONES M ,  LUCA A DE ,  CREACH E

Inventor： DE LUCA A ,  CREACH E ,  JONES M

IPC: F17C13/08 ,  B65D87/00

CPC classification number: F17C13/084 ,  F17C2201/0109 ,  F17C2201/0119 ,  F17C2201/035 ,  F17C2205/0165 ,  F17C2205/0338 ,  F17C2250/043

Abstract: This holder and transporter is for one or more tanks of the type containing oxygen and acetylene gases. Basically, it comprises a base, a handle and means connecting them, the latter serving to receive and secure at least one tank to the assembly. More specifically, the base is of channel shape to provide rigidity, the tank receiving means being a split band to encircle the tank and provide separated ends which are perforated to receive a fastener used in clamping the band to the tank, one of the ends having a handle formed thereon. The handle is offset relative to the band to provide a selected balance between the handle and the tank. If desired, the base may be shaped to give increased strength to light-weight material and provide spaced supporting surface engaging portions which make the holder stable on uneven surfaces. The holder may be adapted for use on a plurality of tanks.

Abstract translation: 该支架和运输器用于一个或多个包含氧气和乙炔气体的罐。 基本上，它包括底座，手柄和连接它们的装置，后者用于接收和固定至少一个罐到组件。 更具体地说，底座具有通道形状以提供刚性，油箱接收装置是分隔带以环绕罐并且提供分开的端部，其被穿孔以接收用于将带夹紧到罐中的紧固件，其中一端具有 形成在其上的手柄。 手柄相对于带偏移，以在手柄和罐之间提供选定的平衡。 如果需要，基底可以成形为对轻质材料提供增强的强度，并提供间隔开的支撑表面接合部分，这使得保持器在不平坦表面上稳定。 保持器可适于在多个罐上使用。

公开(公告)号：US3763341A

公开(公告)日：1973-10-02

申请号：US3763341D

申请日：1972-03-09

Applicant: CLYMER F ,  WHITING J ,  DION W ,  JONES M ,  DALLON R

Inventor： WILLIS G

IPC: H01H3/46 ,  H01H67/26 ,  H01H3/00

CPC classification number: H01H3/46 ,  H01H67/26

Abstract: The switching system of this invention includes a plurality of coordinate contact sets arranged in rows and columns, actuating means corresponding to each column for selectively actuating a contact selector bar corresponding to each row when the contact selector bar has been operated to transmit contact closing and opening forces thereto, and a contact operator at each coordinate operable by the selector bar to effect movement and an automatic latching of a selected contact set.

Abstract translation: 本发明的切换系统包括以行和列布置的多个坐标触点组，对应于每个列的致动装置，用于当接触选择杆已经被操作以传递触点闭合和打开时选择性地启动对应于每一行的触点选择杆 以及每个坐标处的接触操作者，其可由选择杆操作以实现所选择的触点组的移动和自动锁定。

公开(公告)号：US10167494B2

公开(公告)日：2019-01-01

申请号：US12589968

申请日：2009-10-30

Applicant: John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

Inventor： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC: C12Q1/00 ,  C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/3577

Abstract: The present invention provides a method and system for monitoring, detecting, and/or characterizing a biological particle that may be present in a sample. The method may be accomplished in a sealed container by utilizing a first step time-dependent spectroscopic technique to obtain at least two measurements of a growth composition comprising a sample and correlating said measurements for the detection and/or characterization of a biological particle that may be present in the sample. The method further provides for a subsequent step for the separation, characterizion and/or identification of the microorganisms in the sealed container.

公开(公告)号：US10059975B2

公开(公告)日：2018-08-28

申请号：US12589929

申请日：2009-10-30

Applicant: John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

Inventor： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC: C12Q1/04 ,  G01N21/65 ,  G01N33/68 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/47 ,  G01N21/64 ,  G01N21/359

CPC classification number: C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

Abstract: The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

公开(公告)号：US20100129858A1

公开(公告)日：2010-05-27

申请号：US12589952

申请日：2009-10-30

Applicant: John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

Inventor： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay ,  Christopher Ronsick

IPC: C12Q1/04

CPC classification number: C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848 ,  Y02A50/55

Abstract: The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides for spectroscopic interrogation of the separated microorganism sample to produce measurements of the microorganism and characterizing and/or identifying the microorganism in the sample using said spectroscopic measurements.

Abstract translation: 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供分离的微生物样品的光谱询问以产生微生物的测量，并使用所述光谱测量来表征和/或鉴定样品中的微生物。

公开(公告)号：US20100129857A1

公开(公告)日：2010-05-27

申请号：US12589929

申请日：2009-10-30

Applicant: John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

Inventor： John Walsh ,  Jones M. Hyman ,  Thurman Thorpe ,  Bradford Clay

IPC: C12Q1/04 ,  C12N1/00

CPC classification number: C12Q1/04 ,  G01N21/35 ,  G01N21/3581 ,  G01N21/359 ,  G01N21/47 ,  G01N21/64 ,  G01N21/65 ,  G01N33/6848

Abstract: The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The invention further provides methods for separating, characterizing and/or identifying microorganisms in situ within a single system.

Abstract translation: 本发明涉及一种用于分离，表征和/或鉴定测试样品中的微生物的方法。 本发明的方法包括用于裂解可能存在于测试样品中的非微生物细胞的任选裂解步骤，随后是随后的分离步骤。 该方法可用于从诸如含血培养基的复杂样品中分离，表征和/或鉴定微生物。 本发明还提供了在单个系统内原位分离，表征和/或鉴定微生物的方法。