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公开(公告)号：US20100098725A1
公开(公告)日：2010-04-22
申请号：US12566048
申请日：2009-09-24
Applicant: Jonathan LIU , Mark THOMPSON , Luis J. MARANGA , Floro CATANIAG , Simon S. HSU
Inventor： Jonathan LIU , Mark THOMPSON , Luis J. MARANGA , Floro CATANIAG , Simon S. HSU
IPC: A61K39/145 , C12N7/00 , C12N7/02 , A61P37/04 , A61P31/20
CPC classification number: C12N7/02 , A61K39/12 , A61K39/145 , C12N7/00 , C12N2760/16051 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251
Abstract: The present invention provides novel serum-free cell culture medium and methods for cultivating MDCK cells. In particular, non-tumorigenic MDCK cells. The present invention also provides methods for producing influenza viruses (e.g., particularly cold-adapted, and/or temperature sensitive, and/or attenuated influenza viruses) that eliminate the need for a cell culture medium exchange step. The novel medium and methods are useful to grow influenza viruses, in cell culture to high titer. The present invention further provides purification methods for purifying influenza viruses with high overall recovery of live virus and result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.
Abstract translation: 本发明提供新型的无血清细胞培养基和培养MDCK细胞的方法。 特别是非致瘤性MDCK细胞。 本发明还提供了消除对细胞培养基交换步骤的需要的用于产生流感病毒（例如特别冷适应和/或温度敏感和/或减毒的流感病毒）的方法。 新型培养基和方法可用于在细胞培养物中高效滴加流感病毒。 本发明还提供了用于纯化具有高活性病毒总体回收率的流感病毒的纯化方法，并导致宿主细胞DNA（HCD），宿主细胞蛋白（HCP）和非特异性内切核酸酶（例如，Benzonase）的水平，其低于 监管机构要求的规格。 免疫原性组合物可用于主动免疫受试者或产生用于多种用途的抗体，包括被动免疫和诊断性免疫测定。
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公开(公告)号：US20090318310A1
公开(公告)日：2009-12-24
申请号：US12427409
申请日：2009-04-21
Applicant: Changsheng Jonathan Liu , Yiqiong Wu , Kevin Jay LeVan
Inventor： Changsheng Jonathan Liu , Yiqiong Wu , Kevin Jay LeVan
CPC classification number: G06F19/22
Abstract: Certain embodiments of the invention provide systems and methods for the automated assembly of DNA sequence data into contiguous DNA segments using a computer a system. DNA sequence data is entered into the system. The system indexes and groups a plurality of DNA fragment reads utilizing an anchor sequence and consolidates the fragments into larger sequences by merging the fragment reads within a group.
Abstract translation: 本发明的某些实施方案提供了使用计算机系统将DNA序列数据自动装配到连续DNA片段中的系统和方法。 DNA序列数据输入到系统中。 该系统使用锚定序列指数和组合多个DNA片段读取，并通过合并一组中的片段读取将片段合并成更大的序列。
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23.发明申请Transferring a video frame from memory into an on-chip buffer for video processing 审中-公开将视频帧从存储器传输到片上缓冲区进行视频处理公开(公告)号：US20060092320A1
公开(公告)日：2006-05-04
申请号：US10977057
申请日：2004-10-29
Applicant: Brian Nickerson , Samuel Wong , Sunil Chaudhari , Jonathan Liu , Sreenath Kurupati
Inventor： Brian Nickerson , Samuel Wong , Sunil Chaudhari , Jonathan Liu , Sreenath Kurupati
CPC classification number: H04N7/0125 , G09G5/391 , G09G5/395 , G09G2340/0407 , H04N7/0135
Abstract: A portion of a video frame is transferred via a memory burst transfer, from memory to an on-chip buffer. The on-chip buffer has a width that is the same as the memory burst width for the memory. Video processing is performed upon the transferred portion. Other embodiments are also described and claimed.
Abstract translation: 视频帧的一部分通过存储器突发传送从存储器传输到片上缓冲器。 片上缓冲区的宽度与存储器的内存突发宽度相同。 在传送部分执行视频处理。 还描述和要求保护其他实施例。
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24.发明申请公开(公告)号：US20060062489A1
公开(公告)日：2006-03-23
申请号：US10947821
申请日：2004-09-22
Applicant: Samuel Wong , Sreenath Kurupati , Brian Nickerson , Sunil Chaudhari , Jonathan Liu
Inventor： Samuel Wong , Sreenath Kurupati , Brian Nickerson , Sunil Chaudhari , Jonathan Liu
CPC classification number: G06T3/4007
Abstract: A method and apparatus for hardware-based anamorphic video scaling. In one embodiment, the method includes the fetch of zero or more new input pixels according to an entry of an input control memory corresponding to a current output pixel. Once fetched, the zero or more new input pixels replace at least one stored input pixel of N, input pixels. Using the updated N, input pixels and an N, coefficient set selected according to an entry of a coefficient memory corresponding to the current output pixel, a pixel computation, such as, for example, an anamorphic scaling computation, is performed. In one embodiment, the anamorphic scaling is performed by subdividing an X×Y pixel frame into X/M M×Y pixel subframes. Other embodiments are described and claimed.
Abstract translation: 一种用于基于硬件的变形视频缩放的方法和装置。 在一个实施例中，该方法包括根据对应于当前输出像素的输入控制存储器的条目获取零个或多个新的输入像素。 一旦获取，零个或多个新的输入像素代替N个输入像素的至少一个存储的输入像素。 使用根据与当前输出像素相对应的系数存储器的条目选择的更新的N个输入像素和N个系数集合，执行诸如变形缩放计算的像素计算。 在一个实施例中，通过将X×Y像素帧细分为X / M M×Y像素子帧来执行变形缩放。 描述和要求保护其他实施例。
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公开(公告)号：US20170097428A1
公开(公告)日：2017-04-06
申请号：US15251313
申请日：2016-08-30
Applicant: HONGCHUAN SUN , ERIC G. WILDERMUTH , JONATHAN LIU , REESHIDEV BANSAL , SPYRIDON K. LAZARATOS
Inventor： HONGCHUAN SUN , ERIC G. WILDERMUTH , JONATHAN LIU , REESHIDEV BANSAL , SPYRIDON K. LAZARATOS
CPC classification number: G01V1/282 , G01V1/303 , G01V1/362 , G01V2210/512 , G01V2210/584 , G01V2210/74
Abstract: A method, including: obtaining a velocity model generated by an acoustic full wavefield inversion process; generating, with a computer, a variable Q model by applying pseudo-Q migration on processed seismic data of a subsurface region, wherein the velocity model is used as a guided constraint in the pseudo-Q migration; and generating, with a computer, a final subsurface velocity model that recovers amplitude attenuation caused by gas anomalies in the subsurface region by performing a visco-acoustic full wavefield inversion process, wherein the variable Q model is fixed in the visco-acoustic full wavefield inversion process.
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Patent Agency Ranking
公开(公告)号：US09085753B2
公开(公告)日：2015-07-21
申请号：US13473494
申请日：2012-05-16
Applicant: Jonathan Liu , Mark Thompson , Luis J. Maranga , Floro Cataniag , Simon S. Hsu
Inventor： Jonathan Liu , Mark Thompson , Luis J. Maranga , Floro Cataniag , Simon S. Hsu
IPC: C12N7/02 , A61K39/145
CPC classification number: C12N7/02 , A61K39/12 , A61K39/145 , C12N7/00 , C12N2760/16051 , C12N2760/16134 , C12N2760/16151 , C12N2760/16234 , C12N2760/16251
Abstract: The present invention provides novel serum-free cell culture medium and methods for cultivating MDCK cells. In particular, non-tumorigenic MDCK cells. The present invention also provides methods for producing influenza viruses (e.g., particularly cold-adapted, and/or temperature sensitive, and/or attenuated influenza viruses) that eliminate the need for a cell culture medium exchange step. The novel medium and methods are useful to grow influenza viruses, in cell culture to high titer. The present invention further provides purification methods for purifying influenza viruses with high overall recovery of live virus and result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.
Abstract translation: 本发明提供新型的无血清细胞培养基和培养MDCK细胞的方法。 特别是非致瘤性MDCK细胞。 本发明还提供了消除对细胞培养基交换步骤的需要的用于生产流感病毒（例如，特别冷适应的和/或温度敏感的和/或减毒的流感病毒）的方法。 新型培养基和方法可用于在细胞培养物中高效滴加流感病毒。 本发明还提供了用于纯化具有高活性病毒总体回收率的流感病毒的纯化方法，并导致宿主细胞DNA（HCD），宿主细胞蛋白（HCP）和非特异性内切核酸酶（例如，Benzonase）的水平，其低于 监管机构要求的规格。 免疫原性组合物可用于主动免疫受试者或产生用于多种用途的抗体，包括被动免疫和诊断性免疫测定。
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公开(公告)号：US20140316255A1
公开(公告)日：2014-10-23
申请号：US14241790
申请日：2012-08-23
Applicant: Ellis Garai , Cristina Zavaleta , Michael Mandella , Jonathan Liu , Sanjiv Sam Gambhir , Christopher H. Contag
Inventor： Ellis Garai , Cristina Zavaleta , Michael Mandella , Jonathan Liu , Sanjiv Sam Gambhir , Christopher H. Contag
CPC classification number: A61B5/0075 , A61B1/07 , A61B5/0079 , A61B5/0084 , A61B10/02 , A61B34/20 , A61B2034/2057 , A61K49/0095 , B82Y15/00 , G01J1/0433 , G01J3/0213 , G01J3/0218 , G01J3/18 , G01J3/2803 , G01J3/44 , G01N21/658
Abstract: In accordance with the purpose(s) of the present disclosure, as embodied and broadly described herein, embodiments of the present disclosure, in one aspect, relate to Raman imaging devices (e.g., Raman endoscope probes) or systems, methods of using Raman agents, Raman imaging devices, and/or systems to image or detect a signal, and the like.
Abstract translation: 根据本公开的目的，如本文所体现和广泛描述的，本公开的实施例在一个方面涉及拉曼成像装置（例如，拉曼内窥镜探针）或系统，使用拉曼剂的方法 ，拉曼成像装置和/或用于对信号进行成像或检测的系统等。
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28.发明授权MDCK cell lines supporting viral growth to high titers and bioreactor process using the same 有权支持病毒生长至高滴度的MDCK细胞系和使用其的生物反应器过程公开(公告)号：US08846032B2
公开(公告)日：2014-09-30
申请号：US13595897
申请日：2012-08-27
Applicant: Jonathan Liu , Richard Schwartz , Mark Thompson , Luis Maranga , Mridul Ghosh , Ajit Subramanian , Simon Sheng-Tsiung Hsu
Inventor： Jonathan Liu , Richard Schwartz , Mark Thompson , Luis Maranga , Mridul Ghosh , Ajit Subramanian , Simon Sheng-Tsiung Hsu
CPC classification number: C12N7/00 , C12N2760/16151 , C12N2760/16152 , C12N2760/16251 , C12N2760/16252
Abstract: The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.
Abstract translation: 本发明涉及可以在细胞培养中将病毒（例如流感病毒）生长至比先前可能更高滴度的新型MDCK细胞。 MDCK细胞可适应于无血清培养基。 本发明还涉及包含MDCK细胞的细胞培养组合物和用于培养MDCK细胞的培养方法。 本发明还涉及使用本发明的MDCK细胞在细胞培养中生产流感病毒的方法。
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29.发明申请MDCK CELL LINES SUPPORTING VIRAL GROWTH TO HIGH TITERS AND BIOREACTOR PROCESS USING THE SAME 有权使用相同的MDCK细胞系支持病毒生长到高TITERS和生物反应器过程公开(公告)号：US20130052717A1
公开(公告)日：2013-02-28
申请号：US13595897
申请日：2012-08-27
Applicant: Jonathan LIU , Richard SCHWARTZ , Mark THOMPSON , Luis Jorge Camilo MARANGA , Simon Sheng-Tsiung HSU , Mridul GHOSH , Ajit SUBRAMANIAN
Inventor： Jonathan LIU , Richard SCHWARTZ , Mark THOMPSON , Luis Jorge Camilo MARANGA , Simon Sheng-Tsiung HSU , Mridul GHOSH , Ajit SUBRAMANIAN
CPC classification number: C12N7/00 , C12N2760/16151 , C12N2760/16152 , C12N2760/16251 , C12N2760/16252
Abstract: The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.
Abstract translation: 本发明涉及可以在细胞培养中将病毒（例如流感病毒）生长至比先前可能更高滴度的新型MDCK细胞。 MDCK细胞可适应于无血清培养基。 本发明还涉及包含MDCK细胞的细胞培养组合物和用于培养MDCK细胞的培养方法。 本发明还涉及使用本发明的MDCK细胞在细胞培养中生产流感病毒的方法。
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30.发明申请MDCK CELLS LINES SUPPORTING VIRAL GROWTH TO HIGH TITERS AND BIOREACTOR PROCESS USING THE SAME 失效MDCK细胞系支持病毒生长到高TITERS和生物反应器过程使用它公开(公告)号：US20100112669A1
公开(公告)日：2010-05-06
申请号：US12652557
申请日：2010-01-05
Applicant: Jonathan LIU , Richard SCHWARTZ , Mark THOMPSON , Luis Jorge Camilo MARANGA , Simon Sheng-Tsiung Hsu , Mridul GHOSH , Ajit SUBRAMANIAN
Inventor： Jonathan LIU , Richard SCHWARTZ , Mark THOMPSON , Luis Jorge Camilo MARANGA , Simon Sheng-Tsiung Hsu , Mridul GHOSH , Ajit SUBRAMANIAN
CPC classification number: C12N7/00 , C12N2760/16151 , C12N2760/16152 , C12N2760/16251 , C12N2760/16252
Abstract: The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.
Abstract translation: 本发明涉及可以在细胞培养中将病毒（例如流感病毒）生长至比先前可能更高滴度的新型MDCK细胞。 MDCK细胞可适应于无血清培养基。 本发明还涉及包含MDCK细胞的细胞培养组合物和用于培养MDCK细胞的培养方法。 本发明还涉及使用本发明的MDCK细胞在细胞培养中生产流感病毒的方法。
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