公开(公告)号：US5789559A

公开(公告)日：1998-08-04

申请号：US279823

申请日：1994-07-25

申请人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert Tam ,  Kirk E. Fry

发明人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert Tam ,  Kirk E. Fry

IPC分类号： A61K38/00 ,  A61K39/00 ,  C07K14/08 ,  C07K16/10 ,  C12Q1/70 ,  G01N33/576 ,  C07H21/04 ,  C12N15/00 ,  G01N33/20

CPC分类号： C07K14/005 ,  C07K16/10 ,  C12Q1/707 ,  G01N33/5767 ,  A61K38/00 ,  A61K39/00 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2760/10122 ,  C12N2770/00022 ,  C12N2770/24222 ,  C12N2770/28122 ,  Y10T436/143333

摘要： Nucleic acid sequences derived from enterically transmitted nonA/nonB viral hepatitis agent (HEV) are disclosed. DNA sequences encoding specific epitopes within viral protein sequences that are reactive with sera of individuals infected with different strains of HEV are also disclosed. These DNA sequences and fragments thereof are useful for identifying and sequencing the entire viral agent and for assaying the presence of the viral agent in an infected sample, for example by using specific amplification of virus-derived DNA sequences, as well as for producing viral proteins or polypeptides.

摘要翻译： 公开了衍生自肠道传播的非A /非B型肝炎病毒（HEV）的核酸序列。 还公开了编码病毒蛋白质序列中与HEV感染的个体的血清反应的特异性表位的DNA序列。 这些DNA序列及其片段可用于鉴定和测序整个病毒剂并用于测定感染样品中病毒剂的存在，例如通过使用病毒衍生的DNA序列的特异扩增，以及产生病毒蛋白 或多肽。

公开(公告)号：US5766840A

公开(公告)日：1998-06-16

申请号：US466033

申请日：1995-06-05

申请人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

发明人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

IPC分类号： A61K38/00 ,  C07K14/18 ,  C12N1/19 ,  C12N1/21 ,  C12P21/08 ,  C12Q1/70 ,  C07K16/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2770/24222 ,  C12N2770/36022 ,  Y10S530/826

摘要： Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.

公开(公告)号：US5744131A

公开(公告)日：1998-04-28

申请号：US476876

申请日：1995-06-07

申请人： Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

发明人： Cynthia A. Edwards ,  Kirk E. Fry ,  Charles R. Cantor ,  Beth M. Andrews

IPC分类号： C07K14/47 ,  C12N15/09 ,  C12N15/10 ,  C12N15/67 ,  C12Q1/68 ,  C12Q1/70 ,  A61K31/74 ,  G01N33/558 ,  G01N33/566

CPC分类号： C12N15/67 ,  C07K14/47 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q1/6883 ,  C12Q1/705

摘要： The present invention defines an assay useful for screening libraries of synthetic or biological compounds for their ability to bind specific DNA test sequences. The assay is also useful for determining the sequence specificity and relative DNA-binding affinity of DNA-binding molecules for any particular DNA sequence. Also described herein are potential applications of the assay, including: 1) the detection of lead compounds or new drugs via the mass screening of libraries of synthetic or biological compounds (i.e., fermentation broths); 2) the design of sequence-specific DNA-binding drugs comprised of homo- or hetero-meric subunits of molecules for which the sequence specificity was determined using the assay; and 3) the use of molecules for which sequence specificity was determined using the assay as covalently attached moieties to aid in the binding of nucleic acid or other macromolecular polymers to nucleic acid sequences.

公开(公告)号：US08148067B2

公开(公告)日：2012-04-03

申请号：US11938227

申请日：2007-11-09

申请人： Preeti G. Lal ,  Gavin E. Williams ,  Kirk E. Fry ,  Jingtao Sun ,  Russell L. Dedrick

发明人： Preeti G. Lal ,  Gavin E. Williams ,  Kirk E. Fry ,  Jingtao Sun ,  Russell L. Dedrick

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6809 ,  C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/112

摘要： The invention presents a method of diagnosing or monitoring the status of systemic lupus erythematosus (SLE) in a subject or patient comprising detecting the expression of all genes of a diagnostic set in the subject or patient wherein the diagnostic set comprises two or more genes having expression correlated with the classification or status of SLE; and diagnosing or monitoring the status of SLE in the subject or patient by applying at least one statistical method to the expression of the genes of the diagnostic set.

摘要翻译： 本发明提供了诊断或监测受试者或患者的系统性红斑狼疮（SLE）状态的方法，包括检测受试者或患者中诊断集合的所有基因的表达，其中诊断组包含两个或多个具有表达的基因 与SLE的分类或状态相关; 并通过对诊断集合的基因的表达应用至少一种统计方法来诊断或监测受试者或患者中SLE的状态。

公开(公告)号：US07094406B2

公开(公告)日：2006-08-22

申请号：US09851410

申请日：2001-05-07

申请人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert W. Tam ,  Kirk E. Fry

发明人： Gregory R. Reyes ,  Patrice O. Yarbough ,  Daniel W. Bradley ,  Krzysztof Z. Krawczynski ,  Albert W. Tam ,  Kirk E. Fry

IPC分类号： A61K39/29 ,  C07K4/02 ,  C07K7/00 ,  C07K14/08

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07K16/10 ,  C07K2319/00 ,  C07K2319/40 ,  C07K2319/61 ,  C12N2760/10122 ,  C12N2770/00022 ,  C12N2770/24222 ,  C12N2770/28122 ,  C12Q1/707 ,  G01N33/5767 ,  Y10T436/143333

摘要： Viral proteins derived from an enterically transmitted non-A/non-B viral hepatitis agent (HEV) are disclosed. In one embodiment, the protein is immunologically reactive with antibodies present in individuals infected with the viral hepatitis agent. This protein is useful in a diagnostic method for detecting infection by the enterically transmitted agent. Specific epitopes have been identified that are reactive with sera of individual infected with different strains of HEV. Also disclosed are DNA probes derived from a cloned sequence of the viral agent. These probes are useful for identifying and sequencing the entire viral agent and for assaying the presence of the viral agent in an infected sample, by using probe-specific amplification of virus-derived DNA fragments.

公开(公告)号：US5849532A

公开(公告)日：1998-12-15

申请号：US464134

申请日：1995-06-06

申请人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

发明人： Jungsuh P. Kim ,  Kirk E. Fry ,  LaVonne Marie Young ,  Jeffrey M. Linnen ,  John Wages

IPC分类号： A61K38/00 ,  C07K14/18 ,  C12N1/19 ,  C12N1/21 ,  C12P21/08

CPC分类号： C07K14/005 ,  A61K38/00 ,  C12N2770/24222 ,  C12N2770/36022 ,  Y10S530/826

摘要： Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.