中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

31 records (took 0.018 seconds)

    Granule containing enzyme, corn starch and sugar layered on an inert particle
    23.
    发明授权
    Granule containing enzyme, corn starch and sugar layered on an inert particle 有权
    含有颗粒的酶，玉米淀粉和糖分层在惰性颗粒上

    公开(公告)号：US06790643B2

    公开(公告)日：2004-09-14

    申请号：US10180785

    申请日：2002-06-25

    Applicant: Nathaniel T. Becker ,  Thomas S. Green

    Inventor： Nathaniel T. Becker ,  Thomas S. Green

    IPC: C12N998

    CPC classification number: C12N9/98 ,  Y10S530/812 ,  Y10S530/813

    Abstract: Granules that include a protein core are described. The protein core includes a protein matrix which includes a protein mixed together with a starch and optionally sugar such as sucrose. The protein matrix can be layered over a seed particle or the protein core can be homogeneous. The protein can be an enzyme or a therapeutic protein. A barrier layer may surround the protein core, and a coating can be applied to the seed particle, the protein matrix and/or the barrier layer.

    Abstract translation: 描述了包含蛋白质核心的颗粒。 蛋白质核心包括蛋白质基质，其包括与淀粉和任选的糖如蔗糖一起混合的蛋白质。 蛋白质基质可以分层在种子颗粒上，或蛋白质核心可以是均质的。 蛋白质可以是酶或治疗性蛋白质。 阻挡层可以围绕蛋白质核心，并且涂层可以施加到种子颗粒，蛋白质基质和/或阻挡层。

    Granule containing protein and salt layered on an inert particle
    25.
    发明授权
    Granule containing protein and salt layered on an inert particle 有权

    公开(公告)号：US06423517B1

    公开(公告)日：2002-07-23

    申请号：US09215086

    申请日：1998-12-18

    Applicant: Nathaniel T. Becker ,  Robert I. Christensen, Jr. ,  Ernst H. Gros

    Inventor： Nathaniel T. Becker ,  Robert I. Christensen, Jr. ,  Ernst H. Gros

    IPC: C12N998

    Abstract: Granules are prepared containing an admixture of protein and salt layered over an inert particle. A preferred amount of salt is about between 63.7 and 84.3% of the total weight of the admixture. Proteins include pharmaceutically important proteins such as hormones, or industrially important proteins such as enzymes including proteases, amylases, lipases and cellulases capable of hydrolyzing substrates such as stains. Inert particles include inorganic salts, sugars, sugar alcohols, small organic molecules such as organic acids or salts, and minerals such as clays or silicates. A binder such as starch or polyethylene oxide may be mixed in with the admixture. A barrier material such as an inorganic salt or organic acid or salt may be in the admixture or coated over the admixture layer. A coating layer of a soluble or water dispersible film-forming polymer may be between the inert particle and admixture layer and/or over the admixture layer. The granules may also contain plasticizers, extenders, lubricants, pigments and anti-agglomeration agents. A preferred method for preparing the granules is by spraying a solution or slurry of the admixture onto the inert particles while fluidized in a fluid-bed coater.

    Process for making dust-free enzyme-containing particles from an enzyme-containing fermentation broth
    26.
    发明授权
    Process for making dust-free enzyme-containing particles from an enzyme-containing fermentation broth 失效
    从含酶发酵液中制备无尘含酶颗粒的方法

    公开(公告)号：US5814501A

    公开(公告)日：1998-09-29

    申请号：US390264

    申请日：1995-02-15

    Applicant: Nathaniel T. Becker ,  Richard P. Crowley

    Inventor： Nathaniel T. Becker ,  Richard P. Crowley

    IPC: C11D3/386 ,  C12N11/10 ,  C12N11/14 ,  C12N11/00 ,  C11D7/42 ,  C12N9/98

    CPC classification number: C12N11/14 ,  C11D3/38672 ,  C12N11/10

    Abstract: Substantially dust-free enzyme-containing particles are prepared by spray-coating solid particulate cores with a fermentation broth containing enzymes. Fermentation broths containing from 5% to 100% total enzyme solids and 4%-55% total solids content in the broth may be used. For broths containing 4%-31% total solids, enzyme and coating agents may be spray-coated so as to achieve a weight gain of 1%-24% w/w over the initial weight of the cores. For broths with 31%-55% total solids, a corresponding weight gain of 1%-100% may be achieved. The enzyme coated cores may be further coated with coating agents. The total dry weight added to the cores ranges from 1% to 19% in the absence of coating agents and 1% to 24% in the presence of coating agents when the lower range of total solids content is used. The total dry weight added to the cores ranges from 1% to 100% when the higher range of total solids content is used. These conditions are especially useful in the granulation of enzymes with very high specific activities, so that effective enzyme activity can be provided by adding low amounts of broth solids to the cores. Reduction in added solids is of significant economic benefit in reducing granulation costs.

    Abstract translation: 通过用含酶的发酵液喷雾固体颗粒核来制备基本上无尘的含酶颗粒。 可以使用含有5％至100％总酶固体的发酵液和在培养液中的总固体含量为4％-55％的发酵液。 对于含有4％-31％总固体的肉汤，酶和包衣剂可以被喷涂，以便在芯的初始重量下实现1％-24％w / w的体重增加。 对于具有31％-55％总固体的肉汤，可以实现1％-100％的相应增重。 酶包衣的核心可以进一步用包衣剂包衣。 当使用总固体含量的较低范围时，添加到芯的总干重在不存在涂布剂的情况下为1％至19％，在涂覆剂存在下为1％至24％。 当使用更高的总固体含量时，添加到芯中的总干重为1％至100％。 这些条件在具有非常高的比活性的酶的造粒中特别有用，因此可以通过向芯中加入少量的肉汤固体来提供有效的酶活性。 添加固体的减少在减少造粒成本方面具有显着的经济效益。

    Crystalline cellulase and method for producing same
    30.
    发明授权
    Crystalline cellulase and method for producing same 失效
    结晶纤维素酶及其制备方法

    公开(公告)号：US06190898B1

    公开(公告)日：2001-02-20

    申请号：US08609362

    申请日：1996-03-01

    Applicant: Nathaniel T. Becker ,  Edit L. Braunstein ,  Robert Fewkes ,  Ernst H. Gros ,  Meng H. Heng

    Inventor： Nathaniel T. Becker ,  Edit L. Braunstein ,  Robert Fewkes ,  Ernst H. Gros ,  Meng H. Heng

    IPC: C12N914

    CPC classification number: C12N9/2437 ,  C12Y302/01004

    Abstract: A method for preparing a crystalline cellulase enzyme is provided which comprises preparing an aqueous solution containing cellulase enzyme and adding to the aqueous solution a salt comprising an anion selected from the group consisting of sulfate, phosphate, formate, acetate, sorbate, chloride, bromide, fluoride or iodide, and a cation selected from the group consisting of sodium, ammonium, magnesium, potassium or calcium, wherein the aqueous solution is at a temperature between 10° C. and 60° C.

    Abstract translation: 提供了一种制备结晶纤维素酶的方法，其包括制备含有纤维素酶的水溶液，并向该水溶液中加入包含选自硫酸盐，磷酸盐，甲酸盐，乙酸盐，山梨酸盐，氯化物，溴化物， 氟化物或碘化物，以及选自钠，铵，镁，钾或钙的阳离子，其中所述水溶液的温度为10℃至60℃。

Patent Agency Ranking