公开(公告)号：US11384353B2

公开(公告)日：2022-07-12

申请号：US17427040

申请日：2020-02-03

Applicant: ShanghaiTech University

Inventor： Jia Chen ,  Bei Yang ,  Li Yang ,  Xingxu Huang ,  Lijie Wang

IPC: C12N15/10 ,  C12N9/22 ,  C12N9/50 ,  C12N15/11 ,  C12N9/78

Abstract: Provided are fusion proteins and related molecules useful for conducting base editing with reduced or no off-target mutations. The fusion proteins may include a first fragment comprising a nucleobase deaminase or a catalytic domain thereof, a second fragment comprising a nucleobase deaminase inhibitory domain, and a protease cleavage site between the first fragment and the second fragment. Also provided are improved prime editing systems, including prime editing guide RNA with improved stability.

公开(公告)号：US20220148281A1

公开(公告)日：2022-05-12

申请号：US17593852

申请日：2021-06-09

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Hao SUN ,  Yajun HA

IPC: G06V10/22 ,  G06T7/10 ,  G06V20/50

Abstract: An efficient K-nearest neighbor search algorithm for three-dimensional (3D) lidar point cloud in unmanned driving and a use of the foregoing K-nearest neighbor search algorithm in a point cloud map matching process in the unmanned driving are provided. A novel data structure for fast K-nearest neighbor search is used, such that each voxel or sub-voxel includes a proper quantity of points to reduce redundant search. The novel K-nearest neighbor search algorithm is based on a double segmentation voxel structure (DSVS) and a field programmable gate array (FPGA). By means of the novel K-nearest neighbor search algorithm, nearest neighbors are searched for only in a neighboring expected area near a search point, thereby reducing search of redundant points. In addition, an optimized data transmission and access policy is used, which makes the algorithm more fit the characteristic of the FPGA.

公开(公告)号：US20210317480A1

公开(公告)日：2021-10-14

申请号：US17272252

申请日：2019-08-29

Applicant: ShanghaiTech University

Inventor： Jia Liu ,  Biao Jiang ,  Peixiang Ma ,  Guang Yang

IPC: C12N15/90 ,  A61K38/46 ,  A61K38/16 ,  C12N7/00 ,  A61K38/50 ,  A61K31/7088

Abstract: Provided are amino acid sequences capable of binding to and inhibiting a Cas protein's ability to bind to a nucleic acid molecule, thereby inhibiting the Cas protein's function in genome editing. Such Cas protein inhibitors, which can be comprised of a major coat protein (G8P), an extracellular region of the G8P (G8PEX), or a biological equivalent, are useful in improving the specificity of Cas protein-based genome editing procedures.

公开(公告)号：US20210248764A1

公开(公告)日：2021-08-12

申请号：US17054169

申请日：2020-06-17

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Xinzhe LIU ,  Fupeng CHEN ,  Yajun HA

IPC: G06T7/44 ,  G06F9/38 ,  G06F9/54 ,  G06F9/30 ,  G06F1/10

Abstract: An efficient parallel computing method for a box filter, includes: step 1, with respect to a given degree of parallelism N and a radius r of the filter kernel, establishing a first architecture provided without an extra register and a second architecture provided with the extra register; step 2, building a first adder tree for the first architecture and a second adder tree for the second architecture, respectively; step 3, searching the first adder tree and the second adder tree from top to bottom, calculating the pixel average corresponding to each filter kernel by using the first adder tree and the second adder tree, respectively, and counting resources required to be consumed by the first architecture and the second architecture, respectively; and, step 4, selecting one architecture consuming a relatively small resources from the first architecture and the second architecture for computing the box filter.

公开(公告)号：US20210130276A1

公开(公告)日：2021-05-06

申请号：US16478476

申请日：2017-10-26

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Zhiwei ZUO ,  Anhua HU

IPC: C07C45/33 ,  C07C29/50 ,  C07C17/14 ,  C07C281/02 ,  C07C67/307 ,  C07C67/313

Abstract: The present invention provides a direct oxidation method for saturated hydrocarbon bonds in an organic compound. The method allows an organic compound with a saturated hydrocarbon bond to react with an oxidizing reagent in the presence of cerium complex under visible light irradiation, thus oxidizing the saturated hydrocarbon bond to afford an oxidation product. The present reaction only needs to be carried out at room temperature, while the reaction efficiency remains high. In addition, only visible light is required to provide the energy for activation, rendering the present strategy is a milder and greener reaction method. The cerium catalyst used in the method is low in cost, simple and efficient, while the oxidizing reagent used is also stable in nature and low in industrial cost, rendering the catalytic system highly practical. Furthermore, environmental pollution caused by heavy transition metals and peroxides can be avoided in such strategy.

公开(公告)号：US10830741B1

公开(公告)日：2020-11-10

申请号：US16966017

申请日：2018-04-25

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Yong Yang

IPC: G01N1/00 ,  G01N30/72 ,  H01J49/42

Abstract: A coupling structure of a UHV characterization instrument-interconnected in-situ reaction cell and a built-in mass spectrometer electro quadrupole is provided. One end of a stainless steel capillary is connected to a segregated in-situ reaction cell gas output pipeline, and the other end of the stainless steel capillary is a sampling port. A sampling gas flowing out of the sampling port is divided into two gas paths, wherein, one gas path enters a vacuum buffer chamber through a valve with a low flow control ratio, and the other gas path enters a mass spectrometer electro quadrupole through a valve with a high flow control ratio. When the mass spectrometer electro quadrupole performs sampling gas composition analysis on the interconnected in-situ reaction cell, its sampling time delay is negligible and the sampling analysis requirements for in-situ analysis of continuity, real-time and high time resolution are met.

公开(公告)号：US20200074658A1

公开(公告)日：2020-03-05

申请号：US16675617

申请日：2019-11-06

Applicant: Shanghaitech University

Inventor： Jingyi YU

IPC: G06T7/557 ,  G06T17/00 ,  G06T15/20 ,  G06T7/73

Abstract: A method of generating a three-dimensional model of an object is disclosed. The method may use a light field camera to capture a plurality of light field images at a plurality of viewpoints. The method may include capturing a first light field image at a first viewpoint; capturing a second light field image at the second viewpoint; estimating a rotation and a translation of a light field from the first viewpoint to the second viewpoint; obtaining a disparity map from each of the plurality of light field image; and computing a three-dimensional point cloud by optimizing the rotation and translation of the light field and the disparity map. The first light field image may include a first plurality of subaperture images and the second light field image may include a second plurality of subaperture images.

公开(公告)号：US20250163065A1

公开(公告)日：2025-05-22

申请号：US18840522

申请日：2023-01-06

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Jianjun CHENG ,  Zhijie LIU ,  Tian HUA ,  Jianhang MAO ,  Ning ZHOU

IPC: C07D487/04 ,  A61K31/53 ,  A61K31/5377 ,  A61P25/18

Abstract: Provided are a pyrrolotriazinone compound represented by formula I or a pharmaceutically acceptable salt thereof, a pharmaceutical composition comprising same, a preparation method therefor, and use thereof. The pyrrolotriazinone compound has agonistic activity on GPR139 receptor.

公开(公告)号：US20250160308A1

公开(公告)日：2025-05-22

申请号：US18840525

申请日：2023-05-17

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Tian CHI ,  Bo LIU ,  Zhengyu JING ,  Xiaoming ZHANG ,  Shaoshuai MAO ,  Yuxin CHEN

IPC: A01K67/0278 ,  C12N9/12 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: Provided are a nucleic acid construct based on a Cre-LoxP recombination system and a CRISPR gene editing system and use thereof. The Cre-LoxP recombination system comprises a Cre enzyme and a LoxP nucleic acid combination. The LoxP nucleic acid combination comprises TATA-Lox71 and TATA-LoxTC9 sequences, which can only be recombined once under the catalysis of the Cre enzyme. The nucleic acid construct carries an inert “stuffer sequence” with a certain length. The nucleic acid construct can express a plurality of sgRNAs in a low bias manner in vivo, but a same cell can only express one sgRNA, thereby efficiently generating mosaicism whose utilities include accurate and sensitive in-situ CRISPR gene screening and rapid, cost-effective preparation of a single-gene knockout line.

公开(公告)号：US12292946B1

公开(公告)日：2025-05-06

申请号：US18967676

申请日：2024-12-04

Applicant: SHANGHAITECH UNIVERSITY

Inventor： Rui Li ,  Lin Li ,  Yajun Ha

IPC: G06F17/16

Abstract: A method for implementing formal verification of an optimized multiplier via symbolic computer algebra (SCA)-satisfiability (SAT) synergy includes: systematically recovering, by a reverse engineering algorithm, an adder tree from an optimized multiplier; 2) generating, by a constraint satisfaction algorithm, a reference multiplier only by using an adder based on a constraint condition; and 3) combining, by an SCA-based and SAT-based verification method, complementary advantages of SCA and SAT. In the verification framework, the method introduces a reference multiplier generator for generating a correct reference multiplier. The correct reference multiplier has both a structure similar to a structure of the optimized multiplier and a clear adder boundary. The clear adder boundary allows proving correctness of the correct reference multiplier through SCA-based verification. With a structural similarity between the reference multiplier and the optimized multiplier, the reference multiplier is used as a known correct model for SAT-based verification of the optimized multiplier.