公开(公告)号：US11791129B2

公开(公告)日：2023-10-17

申请号：US17435247

申请日：2020-02-20

申请人： Centre National De La Recherche Scientifique ,  Institut National Des Sciences Appliquees De Rouen (INSA) ,  Universite De Rouen Normandie

发明人： François Vurpillot ,  Rodrigue Larde ,  Benjamin Klaes ,  Gérald Da Costa

IPC分类号： H01J37/26 ,  H01J37/285

CPC分类号： H01J37/265 ,  H01J37/285 ,  H01J2237/2626 ,  H01J2237/2852

摘要： A method for imaging a material to atomic scale by means of a field-ion microscope having a vacuum chamber configured to accommodate the material prepared in the form of a tip and an imaging gas, and an ion detector is provided. The method includes application of a DC electrical potential (VDC) and of a pulsed electrical potential, of which the maximum pulse value is denoted Vimp, so that the tip erodes for a potential value equal to VDC+Vimp; acquisition, by the detector between at least two pulses of the pulsed potential, of series of at least two ion images of the impacts of the ions repelled by the tip onto the detector; and calculation of a quantity characteristic of a trend of the erosion of the tip based on the series of ion images acquired and the adjustment, between each series of images, of the values of VDC and of Vimp such that the quantity characteristic of the trend and the ratio VDC/Vimp remain constant.

公开(公告)号：US20230272505A1

公开(公告)日：2023-08-31

申请号：US18313511

申请日：2023-05-08

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  UNIVERSITE DE ROUEN NORMANDIE ,  INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE ROUEN NORMANDIE

发明人： Jean-Marie LE BRETON ,  Nicolas MAAT ,  Cyril AYMONIER

IPC分类号： C22B7/00 ,  C22B59/00 ,  H01F41/02 ,  B01D11/02 ,  B09B3/80 ,  H01F1/057

CPC分类号： C22B7/006 ,  C22B59/00 ,  H01F41/0253 ,  B01D11/02 ,  B09B3/80 ,  B01D11/0207 ,  B01D11/0288 ,  H01F1/0577 ,  Y02P10/20

摘要： The invention concerns a method and a device for retrieving, from an object A, elements G present in a matrix M, characterized in that it comprises at least the following steps: bringing said abject A into contact with a dense fluid Fd with a molar mass greater than 2 g mol−1 under temperature T1 and pressure P1 conditions suitable for transforming the intergranular phase and for releasing the elements G, (302), modifying the temperature T2 and/or pressure P2 values to stop the reaction transforming the intergranular phase, (303), and recovering the elements G separated front the matrix M (304).

公开(公告)号：US11685964B2

公开(公告)日：2023-06-27

申请号：US16318490

申请日：2017-07-17

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  UNIVERSITE DE ROUEN NORMANDIE ,  INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE ROUEN NORMANDIE

发明人： Jean-Marie Le Breton ,  Nicolas Maat ,  Cyril Aymonier

IPC分类号： C22B7/00 ,  C22B59/00 ,  H01F41/02 ,  B01D11/02 ,  B09B3/80 ,  H01F1/057

CPC分类号： C22B7/006 ,  B01D11/02 ,  B01D11/0207 ,  B01D11/0288 ,  B09B3/80 ,  C22B59/00 ,  H01F1/0577 ,  H01F41/0253 ,  Y02P10/20

摘要： Disclosed is a method and a device for retrieving, from an object A, elements G present in a matrix M, the method including at least the following steps: bringing said abject A into contact with a dense fluid Fd with a molar mass greater than 2 g mol−1 under temperature T1 and pressure P1 conditions suitable for transforming the intergranular phase and for releasing the elements G, modifying the temperature T2 and/or pressure P2 values to stop the reaction transforming the intergranular phase, and recovering the elements G separated front the matrix M.

公开(公告)号：US20220290242A1

公开(公告)日：2022-09-15

申请号：US17291407

申请日：2019-11-05

申请人： CENTRE HENRI BECQUEREL ,  UNIVERSITE DE ROUEN-NORMANDIE ,  INSERM (INSTITUT NATIONAL DE LA SANTÉ ET DE LA RECHERCH MÉDICALE)

发明人： Philippe RUMINY ,  Vinciane MARCHAND ,  Ahmad ABDEL SATER ,  Pierre-Julien VIAILLY ,  Marie Delphine LANIC ,  Fabrice JARDIN ,  Marick LAE ,  Mathieu VIENNOT

IPC分类号： C12Q1/6886

摘要： The invention concerns a method for diagnosing a cancer in a subject, comprising a step of RT-MLPA on a biological sample obtained from the subject, in which the RT-MLPA step is carried out using at least one pair of probes comprising at least one probe chosen among the probes with SEQ ID NO: 1 to 13, and/or the probes with SEQ ID NO: 96 to 99, and/or the probes with SEQ ID NO: 866 to 938, and/or the probes with SEQ ID NO: 940 to 1104, and/or SEQ ID NO: 211 to 1312, and/or the probes with SEQ ID NO: 96 to 99, and/or the probes with SEQ ID NO: 1105 to 1107 and/or the probe with SEQ ID NO: 939 and/or the probes with SEQ ID NO: 1108 to 1123, each of the probes being fused, at at least one end, with a priming sequence, and at least one of the probes of the pair comprising a molecular barcode sequence.

公开(公告)号：US11371040B2

公开(公告)日：2022-06-28

申请号：US15769585

申请日：2016-10-21

申请人： Institut National de la Sante et de la Recherche Medicale (INSERM) ,  Universite de Rouen Normandie

发明人： Luca Grumolato

IPC分类号： C12N15/10 ,  C12Q1/6816

摘要： Provided herein are methods and kits for labeling endonuclease-treated cells. The methods comprise: contacting the cells to be labelled with at least one endonuclease suitable for targeting a genomic region of interest, and first and second nucleic acids suitable for introducing one or more silent (or optionally non-silent) mutation(s) in the genomic region by homology-directed repair (HDR). The mutation(s) introduced by the first nucleic acid differ from the mutation(s) introduced by the second nucleic acid.

公开(公告)号：US20220098155A1

公开(公告)日：2022-03-31

申请号：US17292832

申请日：2019-11-14

申请人： UNIVERSITE DE ROUEN NORMANDIE ,  UNIVERSITE DE CAEN NORMANDIE ,  INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE ROUEN (INSA) ,  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS) ,  ECOLE NATIONALE SUPERIEURE D'INGENIEURS DE CAEN

发明人： Vincent REBOUL ,  Xavier FRANCK ,  Thomas GLACHET ,  Hamid MARZAG

IPC分类号： C07D229/02

摘要： The present invention concerns a novel method for synthesising diazirines, that may or may not be enriched in nitro-gen-15, from amino acids or imines, via a one-pot synthesis method, comprising the reaction of the starting amino acid or imine with ammonia, which may or may not be enriched in nitrogen-15, and a hypervalent iodine oxidant. The present invention also relates to a method for synthesising ammonia enriched in nitrogen-15. The invention also concerns certain diazirines of formula (I) likely to be obtained by the claimed synthesis method, and also refers to the 15N2-diazirines of formula (I′). The claimed diazirines can be used in photoaffinity labelling. The 15N2-diazirines can also be used in hyperpolarisation, in particular in the medical imaging field.

公开(公告)号：US20180312831A1

公开(公告)日：2018-11-01

申请号：US15769585

申请日：2016-10-21

申请人： Institute National de la Sante et de la Researche Medicale (INSERM) ,  Universite de Rouen Normandie

发明人： Luca Grumolato

IPC分类号： C12N15/10 ,  C12Q1/6816

CPC分类号： C12Q1/6816 ,  C12Q2563/185

摘要： The instant invention relates to a method for labeling endonuclease-treated cells, comprising the steps of: a) providing cells or a composition comprising cells; b) bringing into contact said cells or said composition with: —at least one endonuclease suitable for targeting a genomic region of interest in said cells, or a vector suitable for expressing said endonuclease in said cells; —at least one first nucleic acid suitable for introducing one or more silent mutation(s) in said genomic region by homology-directed repair (HDR), and optionally one or more non-silent mutation(s); and —at least one second nucleic acid suitable for introducing one or more silent mutation(s) in said genomic region by homology-directed repair (HDR), but distinct from the silent mutations of the first nucleic acid; thereby labeling endonuclease-treated cells.

公开(公告)号：US20180304325A1

公开(公告)日：2018-10-25

申请号：US15767852

申请日：2016-10-13

申请人： CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE ,  UNIVERSITE DE ROUEN-NORMANDIE ,  INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE ROUEN NORMANDIE

发明人： Jean-Marie LE BRETON ,  Nicolas MAÀT ,  Virginie NACHBAUR

IPC分类号： B09B3/00 ,  C01F17/00 ,  B09B5/00 ,  C01G49/08

CPC分类号： B09B3/0083 ,  B09B3/00 ,  B09B5/00 ,  C01F17/0043 ,  C01G49/08 ,  C01P2004/03 ,  C01P2004/10 ,  C01P2004/41

摘要： Disclosed is a method and system for recovering at least rare earth elements from within an object A consisting of at least one first rare earth portion or a mixture of rare earth elements and a second metal portion. The method includes a solvothermal treatment step that places the object in contact with a fluid for causing at least one rare earth portion and/or mixture of rare earth elements and the metal portion to oxidize in order to separate same, the value of the reaction temperature Tr is selected according to the nature of the object, the reaction following a R-M→R(X)x+M(X)y scheme, where R is the rare earth element or a mixture of rare earth elements, M is the transition metal, and (X) is a group which depends on the fluid used.